Streptomyces sennicomposti sp. nov., an actinomycete isolated from compost of Senna siamea (Lam.)

Abstract

A member of the genus Streptomyces , designated RCPT1-4 T , was isolated from compost of Senna siamea (Lam.), collected from an agricultural area in Rayong province, Thailand. The spore morphology and the presence of ll -diaminopimelic acid in the peptidoglycan indicate that RCPT1-4 T shows the typical properties of members of the genus Streptomyces . On the basis of the results of 16S rRNA gene sequence analysis, the strain should be classified as representing a member of the genus Streptomyces and was most closely related to Streptomyces fumigatiscleroticus NBRC 12999 T with the highest 16S rRNA gene sequence similarity of 99.2 %, followed by Streptomyces spiralis NBRC 14215 T (99.0 %). In addition, RCPT1-4 T shared the highest average nucleotide identity by blast (ANIb) (86.0 %), and digital DNA–DNA hybridization (dDDH) (32.1 %) values with S. spiralis NBRC 14215 T . Furthermore, several physiological and biochemical differences were observed between RCPT1-4 T and the closely related type strains of species with validly published names. These taxonomic data indicated that RCPT1-4 T could be considered to represent a novel species of the genus Streptomyces and the name Streptomyces sennicomposti sp. nov. is proposed for this strain. The type strain is RCPT1-4 T (=TBRC 11260 T =NBRC 114303 T ).