A Novel Approach for Optimizing Molecularly Imprinted Polymer Composition in Electrochemical Detection of Collagen Peptides

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dc.contributor.authorVongmanee, Naphatsawan
dc.contributor.authorNampeng, Jindapa
dc.contributor.authorRattanapithan, Katesirin
dc.contributor.authorSriwichai, Phuritasinee
dc.contributor.authorPintavirooj, Chuchart
dc.contributor.authorVisitsattapongse, Sarinporn
dc.date.accessioned2026-08-06T10:52:28Z
dc.date.available2026-08-06T10:52:28Z
dc.date.issued2025-11-01
dc.description.abstractCollagen peptides are key structural proteins that play an important role in maintaining the integrity and proper function of multiple tissues in the human body. Their breakdown is recognized as an important biomarker for various degenerative conditions, including the loss of muscle mass, joint and bone disorders, and compromised skin health. Current analytical approaches for collagen detection, such as ultraviolet spectrometry, enzyme-linked immunosorbent assay (ELISA), high-performance liquid chromatography (HPLC), and histochemical staining, are widely used but often expensive, time-consuming, and reliant on specific laboratory instrumentation, limiting their practicality for routine or rapid diagnostics. This study reports a novel biosensor for collagen peptide detection based on molecularly imprinted polymers (MIPs) integrated with screen-printed electrodes (SPEs). Electrochemical measurements revealed a clear correlation between collagen concentration and current response, confirming effective molecular binding within the imprinted matrix. The optimized MIP-modified electrode exhibited a detection range of 0.1–1000 µg/mL with a limit of detection (LOD) of 1.0106 µg/mL, limit of quantification (LOQ) of 4.46 µg/mL, sensitivity of 8.3816, and correlation coefficient (R<sup>2</sup> = 0.9436). These results highlight strong selectivity and sensitivity toward collagen peptides. The proposed MIP-based biosensor provides a rapid, low-cost platform for detecting collagen degradation products and holds potential for early diagnosis and future clinical applications in degenerative disease monitoring.
dc.identifier.citationBioengineering, 12(11), 2025
dc.identifier.doi10.3390/bioengineering12111272
dc.identifier.issn23065354
dc.identifier.other2-s2.0-105023379637
dc.identifier.urihttps://dspace.kmitl.ac.th/handle/123456789/17320
dc.sourceBioengineering
dc.subjectbiomolecules recognition
dc.subjectbiosensors
dc.subjectcollagen peptides
dc.subjectelectrochemical measurement
dc.subjectmolecular imprinting technique
dc.subjectscreen printed electrode
dc.titleA Novel Approach for Optimizing Molecularly Imprinted Polymer Composition in Electrochemical Detection of Collagen Peptides
dc.typeArticle

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