Production of Coffee oil and Bioactive Peptides from Spent Coffee Grounds via Supercritical Carbon Dioxide Extraction and Enzymatic Hydrolysis

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dc.contributor.authorHunsub, Panusorn
dc.contributor.authorPonmana, Kanokporn
dc.contributor.authorNgamprasertsith, Somkiat
dc.contributor.authorSakdasri, Winatta
dc.contributor.authorKarnchanatat, Aphichart
dc.contributor.authorSawangkeaw, Ruengwit
dc.date.accessioned2026-08-06T10:45:55Z
dc.date.available2026-08-06T10:45:55Z
dc.date.issued2024-04-01
dc.description.abstractSupercritical carbon dioxide (SCCO<inf>2</inf>) extraction was applied for recovering non-polar compounds in spent coffee grounds (SCGs) before enzymatic hydrolysis was performed. The SCCO<inf>2</inf> extracted oil yield of 11.93 wt% was observed at 30 MPa and 50 °C. The detectable volatile compounds in SCGs oil were aldehydes and flavor compounds classified as furans. Although the degree of hydrolysis of SCGs and defatted SCGs (DFSCGs) were not significantly different, the soluble protein of DFSCGs was higher than that of SCGs. Electrophoretic profiles of SCGs and DFSCGs comprise polypeptide bands at < 20 and ~ 24 kDa, which are mainly derived from 11S globulin subunits. After hydrolysis, the molecular masses of 4–20 and 24 kDa were virtually eliminated, thus releasing polypeptides with molecular masses < 4 kDa. Furthermore, the DFSCGs hydrolysate had higher total phenolic content and antioxidant capacity than that of SCGs hydrolysate. Pretreatment of SCGs with SCCO<inf>2</inf> enhances enzymatic accessibility, improves the quality of protein hydrolysate, and procures SCGs oil as a by-product. Graphical Abstract: (Figure presented.)
dc.identifier.citationWaste and Biomass Valorization, 15(4), 2061-2070, 2024
dc.identifier.doi10.1007/s12649-023-02264-8
dc.identifier.issn18772641
dc.identifier.other2-s2.0-85170101166
dc.identifier.urihttps://dspace.kmitl.ac.th/handle/123456789/15587
dc.sourceWaste and Biomass Valorization
dc.subjectAntioxidant activity
dc.subjectBioactive peptides
dc.subjectEnzymatic hydrolysates
dc.subjectSpent coffee grounds
dc.subjectSupercritical carbon dioxide
dc.titleProduction of Coffee oil and Bioactive Peptides from Spent Coffee Grounds via Supercritical Carbon Dioxide Extraction and Enzymatic Hydrolysis
dc.typeArticle

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