Effect of solid-state fermentation using Aspergillus oryzae and Aspergillus niger on bitter and bioactive compounds of Moringa oleifera seed flour

dc.contributor.authorPuspitasari, Candytias
dc.contributor.authorPinsirodom, Praphan
dc.contributor.authorWattanachaisaereekul, Songsak
dc.date.accessioned2026-08-06T10:46:59Z
dc.date.available2026-08-06T10:46:59Z
dc.date.issued2024-09-01
dc.description.abstractMoringa seeds are a valuable source of phenolics and antioxidants when incorporated into food formulations. However, they also have a characteristic bitter taste attributed to the presence of glucosinolate compounds. This study aimed to assess the impact of solid-state fermentation (SSF) with monocultures and co-cultures of Aspergillus oryzae (AO) and Aspergillus niger (AN) on the levels of glucosinolate and phenolic compounds in moringa seed flour. After autoclave sterilization, the most significant reduction of glucosinolates was observed in samples fermented via AO:AN (1:1) co-culture, accompanied by a notable increase in myrosinase activity. Meanwhile, total phenolic and tannin content were most markedly increased with AO:AN (1:2) co-culture following autoclave sterilization. Assays measuring antioxidant activity (DPPH, ABTS, and FRAP) also showed substantial enhancement after fermentation. Pearson Correlation analysis indicated tannins to feature prominently in the antioxidant activity of moringa seed flour. All told, the results demonstrate SSF with AO:AN co-culture to be effective in reducing glucosinolates, elevating phenolic compounds, and enhancing antioxidants, the fermented flour also exhibited a noteworthy increase in protein content. These findings highlight the promising potential of fermented moringa seed flour in developing food products.
dc.identifier.citationLwt, 207, 2024
dc.identifier.doi10.1016/j.lwt.2024.116616
dc.identifier.issn00236438
dc.identifier.other2-s2.0-85201768158
dc.identifier.urihttps://dspace.kmitl.ac.th/handle/123456789/15866
dc.sourceLwt
dc.subjectAntioxidant activity
dc.subjectGlucosinolate
dc.subjectMoringa seed flour
dc.subjectPhenolic compounds
dc.subjectSolid-state fermentation
dc.titleEffect of solid-state fermentation using Aspergillus oryzae and Aspergillus niger on bitter and bioactive compounds of Moringa oleifera seed flour
dc.typeArticle

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