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    Effects of 6 benzylaminopurine and meta topoline on micropropagation of Dendrobium chrysanthum Wall. ex. Lindl
    (2022-09-01)
    Poeaim, A.
    ;
    Kunwanlop, W.
    ;
    Boonmee, W.
    ;
    Laipasu, P.
    The results of solid Gamborg's B5 medium at 2 mg/L mT found the highest average number of shoots at 1.6 shoots per explant; and the number of leaves at 3.7 leaves per explant. The concentration of 1.5 mg/L of mT showed the highest average length of shoots which was 9.82 mm, while the 0.5 mg/L mT revealed the most significant number of roots which were 0.9 roots per explant with the longest root of 3.75 mm. The liquid Gamborg's B5 medium showed the highest average number of shoots at 1.5 mg/L BAP, the same as at 1.5 mg/L mT. The medium without plant growth regulator and 1 mg/L mT was the highest average length of shoots of 13.98 mm. The highest number of roots per explant was 0.8, similar to 2 mg/L mT, but the different maximum length of roots of 3.04 mm. A hundred percent of protocorms induced shoots found in all media. The plantlets were survived after transplanting into pots and covered with plastic bags in four weeks.
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    Effect of plant growth regulators on micropropagation of Vanilla aphylla and Vanilla planifolia sp. Variegate
    (2018-12-01)
    Kunwanlop, W.
    ;
    Boonmee, W.
    ;
    Laipasu, P.
    ;
    Chareonsap, P. P.
    ;
    Krajangvuth, T.
    The plant growth regulators for propagating large number of vanillas in a short period of time was reported. Axillary buds were used from the explants of Vanilla aphylla and Vanilla planifolia sp. variegata that cultured on solid Gamborg's B5 medium supplemented with 0, 0.5, 1, 1.5 and 2 mg/L 6-benzylaminopurine (BA) for 8 weeks. The result showed that the highest average number of shoot proliferation and root induction were obtained at 1 mg/L BA of 3.2 cm. and 5.3 cm. respectively for Vanilla aphylla. Vanilla planifolia sp. variegata showed the highest shoot elongation of 1.9 cm. at 0.5 mg/L BA. Root induction was the highest of 0.9 cm. at 2 mg/L BA. After 12 weeks of culture The explants were grew the best after 12 weeks. They were transplanted into pots and covered by plastic bags for acclimatization and has maintained in greenhouse.