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    The effect of Moina sp. immersion with 17alpha-methyl testosterone (17MT) on growth performances and sex reversal of Nile tilapia
    (2023-07-01)
    Pilapang, K.
    ;
    Srijad, S.
    ;
    The results found that Moina sp. immersion with 17alpha-methyl testosterone (17MT) has a non-effect on the growth performances and non-effect on the sex reversal of Nile tilapia. The higher length, weight, and specific growth rate were found in the PC (PC: 60 mg17MT/kg mixing commercial feed) and the NC (only commercial feed without 17MT) than in other groups (50 and 100 mg17MT/l immersing Moina sp.). The length and the weight of the PC and NC groups showed no significant difference. But these groups were different from the groups of 50 and 100 mg/l. The average length of the PC, NC, 50, and 100 mg/l were displayed at 2.67±0.08, 2.86±0.17, 1.45±0.07 and 1.46±0.02 cm, respectively. The average weight of the PC, NC, 50, and 100 mg/l was displayed as 0.40±0.07, 0.51±0.08, 0.08±0.10 and 0.07±0.01 g, respectively. The highest specific growth rate was found in the NC group (2.37±0.00%). The survival rate of the NC group was not significantly different from other groups. 17MT does not affect growth performances in the larval stage. However, dried Moina sp. immersion in 17MT could be caused an effect on growth performances and sex reversal.
      1
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    Ultrasonic enhancement of lipase-catalyzed transesterification for biodiesel production from used cooking oil
    (2023-06-01)
    Patchimpet, Jaran
    ;
    Zhang, Yi
    ;
    Simpson, Benjamin K.
    ;
    Rui, Xin
    ;
    Sangkharak, Kanokphorn
    This work investigated the ultrasonic irradiation-enhanced transesterification using lipase from the viscera of Nile tilapia (Oreochromis niloticus) to produce biodiesel from used cooking oil (UCO) and methanol. The effects of processing conditions such as irradiation time, ultrasonic frequency, and temperature on biodiesel yield were investigated. Results showed that the use of ultrasound decreased the reaction time from 28 to 3 h with ultrasonic frequency of 16 kHz, methanol to oil molar ratio of 4:1, lipase concentration of 30 kUnit, and reaction temperature of 40 °C. The efficacy of ultrasound was compared with conventional mechanical stirring operated at optimum conditions, and the ultrasonic irradiation coupled with stirring enhanced the transesterification with the highest yield of 97.59%. The fuel properties of the produced biodiesel in this study desirably met the recommended biodiesel standards as prescribed by EN 14214 and ASTM D 6751, suggesting the biodiesel obtained from ultrasound-assisted lipase-catalyzed transesterification of UCO with methanol is a promising alternative for conventional biodiesels and diesels. Graphical abstract: [Figure not available: see fulltext.].
      1
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    Lactiplantibacillus argentoratensis and Candida tropicalis Isolated from the Gastrointestinal Tract of Fish Exhibited Inhibitory Effects against Pathogenic Bacteria of Nile Tilapia
    (2023-02-01)
    Siangpro, Noppadon
    ;
    Chuakrut, Songkran
    ;
    Sirimanapong, Wanna
    ;
    Tanasupawat, Somboon
    ;
    Phongsopitanun, Wongsakorn
    Nile tilapia is one of the most consumed farmed fish in the world. The outbreak of pathogenic bacterial diseases causes high mortality rates and economic losses in Nile tilapia farming. Antibiotic administrations are commonly utilized to inhibit and prevent bacterial infections. However, antibiotics are expensive and cause serious concerns for antibiotic resistance in fish that can be potentially transferred to humans. As an alternative solution, probiotics can be used to prevent infection of pathogenic bacteria in fish. In this work, both bacteria and yeast were isolated from fish gastrointestinal tracts and their inhibitory activity against Nile tilapia pathogenic bacteria was evaluated, as well as other probiotic properties. In this study, 66 bacteria and 176 acid tolerant yeasts were isolated from fish gastrointestinal tracts. Of all isolated microorganisms, 39 bacterial and 15 yeast isolates with inhibitory effect against pathogens were then examined for their probiotic properties (acidic and bile salt resistance, adhesion potential, and biofilm formation), formation of antibacterial factor survival rate under simulated gastrointestinal fluid, and safety evaluation. AT8/5 bacterial isolate demonstrated probiotic properties and the highest inhibition against all 54 tested pathogens while YON3/2 yeast isolate outperformed the inhibitory effect among all yeast isolates. These two probiotic isolates were further identified by 16S rDNA and the D1/D2 domain of 26S rDNA sequence analysis for bacterial and yeast identification, respectively. AT8/5 and YON3/2 showed the highest similarity to Lactiplantibacillus argentoratensis and Candida tropicalis, respectively. This is the first report on isolated L. argentoratensis and C. tropicalis with antipathogenic bacteria of Nile tilapia properties. Collectively, AT8/5 and YON3/2 could be potentially used as promising alternatives to existing antibiotic methods to prevent pathogenic bacteria infection in Nile tilapia farming.
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    In vitro screening of potential probiotic lactic acid bacteria isolated from intestinal contents and gills of Nile tilapia
    (2020-07-01)
    Prachom, N.
    ;
    Rumjuankiat, K.
    ;
    Sanguankiat, A.
    ;
    Boonyoung
    ;
    The potential probiotic lactic acid bacteria (LAB) were isolated from intestinal contents and gills of Nile tilapia (Oreochromis niloticus). Those properties included antagonistic activity against pathogenic bacteria, tolerance to pH, NaCl, fresh bile from Nile tilapia. Moreover, drug resistance profiles were examined. A total of forty isolates were collected. LAB isolated from the intestinal contents showed inhibitory activity against Bacillus coagulans TISTR1447, Pseudomonas fluorescens TISTR358 and Salmonella Typhimurium TISTR292, whereas those from the gills revealed only antagonistic activity against Bacillus coagulans TISTR1447, S. Typhimurium TISTR292 and Escherichia coli TISTR780. Isolates LI10 and LG5 were selected for further evaluation of probiotic properties. Both isolates could grow in pH 4-10, NaCl concentration up to 10% and fresh bile concentration up to 10%. These isolates were resistant to cefovecin, marbofloxacin, clindamycin and trimethoprim/sulfamethoxazole, meanwhile they were susceptible to benzylpenicillin, amoxicillin/clavulanic acid, vancomycin, tetracycline, nitrofurantoin and chloramphenicol. In addition, both isolates were identified by Matrix-assisted lazer desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) as Enterococcus feacalis. Therefore, these two isolates could potentially be used as probiotics.
      1
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    Effects of dietary supplementation with broccoli sprouts (Brassica oleracea) on the hematology and immunological responses of nile tilapia (oreochromis niloticus)
    (2020-01-01) ; ;
    Areechon, Nontawith
    We analyzed the effects of dietary administration of broccoli sprouts (Brassica oleracea) at varying concentrations (0, 10, 15, 20 and 25 gkg<sup>-1</sup> diet, for one and two weeks) on the hematology and select immunological parameters of Nile tilapia (Oreochromis niloticus). Subsequent results showed that the hematocrit level and red blood cell count remained unchanged following ingestion of broccoli sprouts for one and two weeks. However, the fish fed the supplemented diets of 20 and 25 gkg<sup>-1</sup> for two weeks displayed significant increase in white blood cell counts. Further analyses revealed that Nile tilapia fed with 10, 15, 20 and 25 g broccoli sproutskg<sup>-1</sup> for two weeks displayed significant (p<0.05) improvement in phagocytic activity compared to that of the control group. In addition, serum bactericidal activity against Aeromonas hydrophila and Streptococcus agalactiae was enhanced in broccoli sprout-fed groups as early as one week after feeding. These results suggest that broccoli sprouts can enhance immune response in Nile tilapia by increasing total white blood cells and stimulating both cellular and humoral immunity.
      1
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    Changes in lipids and fishy odour development in skin from Nile tilapia (Oreochromis niloticus) stored in ice
    (2013-01-01)
    Sae-Leaw, Thanasak
    ;
    Benjakul, Soottawat
    ;
    Gokoglu, Nalan
    ;
    Changes in lipids, lipoxygenase activity and fishy odour development in the skin of Nile tilapia (Oreochromis niloticus) during iced storage of 18 days were monitored. Triacylglycerol content of skin decreased with coincidental increases in free fatty acid, monoacylglycerol, diacylglycerol and phospholipid contents during storage (p < 0.05). During iced storage, peroxide value increased at day 9 and subsequently decreased up to 18 days (p < 0.05). Thiobarbituric acid reactive substances values and lipoxygenase activity increased throughout 18 days of iced storage (p < 0.05). With increasing storage time, a progressive formation of hydroperoxide was found as evidenced by the increase in amplitude of peak at 3600- 3200 cm-1 in Fourier transform infrared spectra. Those changes indicated that lipid oxidation took place during iced storage. The increase in fishy odour of skin was observed as the storage time increased. The development of fishy odour in Nile tilapia skin during iced storage was mostly governed by lipid oxidation via autoxidation or induced by lipoxygenase. Thus, the extended storage time of whole fish resulted in the pronounced changes in lipids and the increased fishy odour in the skin. © 2013 Elsevier Ltd.
      1
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    Lipid oxidation and fishy odour development in protein hydrolysate from Nile tilapia (Oreochromis niloticus) muscle as affected by freshness and antioxidants
    (2012-06-15)
    Yarnpakdee, Suthasinee
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    Benjakul, Soottawat
    ;
    ;
    Kristinsson, Hordur G.
    Lipid oxidation and fishy odour development in protein hydrolysate from fresh and ice-stored Nile tilapia (Oreochromis niloticus) were investigated. During iced storage of 18 days, heme iron content decreased with a concomitant increase in non-heme iron content (P < 0.05). Peroxide value (PV) and thiobarbituric acid reactive substances (TBARS) values increased. Phospholipid content decreased with a corresponding increase in free fatty acid content. The results suggested that lipid hydrolysis and oxidation took place during storage. When protein hydrolysates were produced from fresh and 18 days ice-stored Nile tilapia muscle, higher lipid oxidation and fishy odour/flavour along with higher amount volatile compounds were obtained in hydrolysate for unfresh sample (P < 0.05). However, the addition of mixed antioxidants during hydrolysis process markedly lowered lipid oxidation, b <sup>·</sup>, ΔC <sup>·</sup>, ΔE <sup>·</sup> values, fishy odour/flavour as well as the formation of volatile compounds in the resulting hydrolysates prepared from both fresh and unfresh samples. Therefore, hydrolysate from Nile tilapia muscle with reduced fishy odour and lighter colour could be prepared by using fresh fish and incorporation of mixed antioxidants during hydrolysis. © 2011 Elsevier Ltd. All rights reserved.
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