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    Assessment of antioxidant enzymes in response to exogenous titanium dioxide (TiO2) nanoparticles in Chainat 1 rice cultivar
    (2018-01-01)
    Samart, Sutichai
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    Antioxidant enzymes are important to prevent the destruction of photosynthetic pigments in plant cells. There have been reported that ZnO nanoparticles can promote activities of antioxidant enzymes in rice, but should not use in high activities because it can cause toxicity. Therefore, the objective of this study was to investigate the effect of titanium dioxide (TiO<inf>2</inf>) nanoparticles on the activities of antioxidant enzymes in Chainat 1 rice cultivar. Rice plants were treated with TiO<inf>2</inf> nanoparticles using different concentrations i.e., 0, 100, 200, 400, 600 and 800 mg.L<sup>-1</sup> for every 7 days-interval. Photosynthetic pigment contents (chlorophyll A, chlorophyll B and carotenoid) and activities of antioxidant enzymes (catalase and peroxidase) were recorded at 37, 44 and 51 days after planting. The results showed that the treatment of TiO<inf>2</inf> nanoparticles was not effect on the quantities of chlorophyll A, chlorophyll B and carotenoids, but found that the activities of peroxidase and catalase enzymes were significant affected at all growth stages. Especially, at a concentration of 200 mg.L<sup>-1</sup> TiO<inf>2</inf> found that rice plants contained the highest activities of peroxidase enzyme and significant difference from control treatment. However, the treatments of higher dose than 200 mg.L<sup>-1</sup> TiO<inf>2</inf> would have a chance to meet toxicity in rice plants as the activities of antioxidant enzymes were decreased.
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    Evaluating the effect of zinc oxide nanoparticles on the physiological responses of nine non-photoperiod sensitive rice cultivars
    (2017-01-01)
    Samart, Sutichai
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    The effects of nanopaticles on plant growth and metabolites have been reported in previous studies, but knowledge of the potential impacts of physiological responses is still unclear. Therefore, this study focused on the effect of exposure of zinc oxide (ZnO) nanoparticles on photosynthetic pigment contents (chlorophyll A, chlorophyll B and carotenoids) and the antioxidant enzyme activities (catalase and peroxidase) in nine non-photoperiod sensitive rice cultivars consisting of RD41, RD49, Chai Nat 1, Suphan Buri 1, Suphan Buri 2, Suphan Buri 3, Pathum Thani 1, Pathum Thani 80 and Phitsanulok 2. Rice plants were treated with 200 mg L<sup>-1</sup> ZnO nanoparticles for every 15 days-interval. Rice plants were collected at 37, 44 and 51 days after planting for the evaluation of ZnO nanoparticles toxicity. The results showed that nine non-photoperiod sensitive rice cultivars treated with ZnO nanoparticle, as determined by the reduction of photosynthetic pigment contents. The activities of antioxidant enzymes protected plant cell when exposed to the toxicity of ZnO nanoparticles. This study showed that the supplementation of ZnO nanoparticles was no significant difference in photosynthetic pigment contents of rice cultivars. The results indicated that the accumulation of antioxidant enzyme activities could be protecting damage of the photosynthetic pigments in plant cells.
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    Item type:Publication,
    High contents of proline and anthocyanin increase protective response to salinity in Oryza sativa L. spp. indica
    (2011-09-01) ;
    Cha-um, Suriyan
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    Pigmented rice cultivar is a good candidate for abiotic tolerance especially salt tolerant trait. Physiological and growth parameters of eight indica rice genotypes under control or salt stress conditions were subjected to Hierarchical cluster analysis. From cluster ranking, rice genotypes were classified into two groups, salt-tolerant (Sangyod, Khao Dang, Kulab Dang and TD49) and salt-sensitive genotypes (Klum Sakol, Klum Khonkaen1, Klum Khonkaen2 and Black Sticky Rice). In salt stress (100 mM NaCl), the salt-tolerant genotypes showed the high percentages of proline and anthocyanin more than in the salt-sensitive genotypes. In addition, the photosynthetic pigment stabilizations of stressed-seedlings were positively correlated to the proline (R <sup>2</sup> = 0.98) and anthocyanin (R <sup>2</sup> = 0.70) accumulations. While, the malondialdehyde (MDA) contents of salt-stressed seedlings were negatively correlated to the proline (R <sup>2</sup> = 0.63) and anthocyanin (R <sup>2</sup> = 0.51) accumulations. Moreover, the total antioxidant activity of extraction from stressed-seedlings was assessed using the ABTS (2,2-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid) method. The higher percentages of total antioxidant activity were determined in salt-tolerant (125-199%) than salt-sensitive genotypes (106-113%). From the results it can be concluded that accumulation of above mentioned metabolites is associated with cellular protection and salt detoxification in salinity rice seedlings.
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