Now showing 1 - 10 of 49
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    N-doped Porous Carbon from Palm Male Flower via Hydrothermal Carbonization
    (2020-07-30)
    Verasarut, Panupong
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    Liamprawat, Tanatorn
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    Kaewtrakulchai, Napat
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    Panomsuwan, Gasidit
    N-doped porous carbon materials were produced from palm male flower using hydrothermal carbonization processes at 200 C for 24 h followed by N-Doping and carbonization at 700C for 2 h. N-doping was carried out by impregnation using NH4OH at 0.5, 1.0, 1.5 M and 2 M. Products were characterized by means of chemical composition and morphology using SEM, XPS, and XRD to characterize specific properties such as physical morpholog, porosity, elemental composition on surface and crystalline structure of PMF. After applying hydrothermal carbonization processes, the results showed substantially increased porosity and surface area with suitable microstructure for N-doped electrodes applications. The highest porosity was obtained at NPC-1.5 M.
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    Biodiversity and bioactivity of stromatic xylariales collected from Mu Ko Chang National Park, Thailand
    (2020-01-01)
    Sutjaritvorakul, T.
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    Ko Chang Island is a tropical rain forest island of Mu Ko Chang national park, Thailand, which has a wide variety of macro-fungi. The objective of this research was to study the biodiversity of Xylariales and to investigate the bioactive activity of ethyl acetate crude extracts. The fungi were collected from the forest area of the waterfalls all over the island. The crude extracts of selected fungi were tested for antibacterial activity by agar well diffusion method and the cancer cell viability was assessed using the MTT assay. Three genera of Xylariaceae and Hypoxylaceae were found including Xylaria, Daldinia and Hypoxylon. The crude extracts inhibited the growth of Gram positive bacteria more than that of Gram negative bacteria and showed broad spectrum inhibition to all cancer cell lines. The crude extract of Xylaria sp.3 showed the highest potential of antibacterial activity and selectively inhibited gastric carcinoma (Kato III) and T cell leukemia (Jurket) with the percentage survival of 26.12 ± 0.90% and 35.37 ± 0.40% respectively. The molecular identification of Xylaria sp.3 was identified as Xylaria sp.TP5BS101. These results show that these macromycetes are an excellent repertoire of antibacterial activity and exhibit anticancer activity against Kato III and Jurket by inducing DNA fragmentation.
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    Modifications of morphological and physiological characteristics of pigmented-rice seedlings by application of titanium dioxide nanoparticles
    (2018-09-05)
    Samart, Sutichai
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    Nanomaterials or metal nanoparticles are considered as the agricultural applications with significant response-promoting influence. This research was focused on the influence of titanium dioxide nanoparticles (TiO<inf>2</inf> NPs) on morphological and physiological processes in pigmented-rice plants at seedling stage. A significant influence of TiO<inf>2</inf> NPs on the plant growth, photosynthetic pigment contents and antioxidant enzyme activities in rice seedlings was investigated. The results were also shown that pigmented-rice seedlings growing in TiO<inf>2</inf> NPs application significantly differed in the morphological and physiological responses when compared with the control plants. The results presented the plant height and dry weight was significantly affected by TiO<inf>2</inf> NPs. The results observed an increase in chlorophyll A, chlorophyll B, carotenoid contents and catalase, peroxidase enzyme activities in plant seedlings of TiO<inf>2</inf> NPs-treated plants which positive-corresponded with plant growth performances was found. TiO<inf>2</inf> NPs-treated seedlings that a high level of TiO<inf>2</inf> NPs application exhibited an increase in the plant growth, photosynthetic pigment contents and antioxidant enzyme activities. The amelioration effect of 800 mg L<sup>-1</sup> of TiO<inf>2</inf> NPs application was shown the highest performances in morphological and physiological responses when compared with the other treatments. Investigation of the results suggests that the morphological and physiological responses due to changes in the plant growth, photosynthetic pigment content and antioxidant enzyme activity parameters may derive from the interaction between TiO<inf>2</inf> NPs and plant cells of pigmented-rice seedlings, which are known to play a role in plant development and productivity in the further study.
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    Valorization of horse manure conversion to magnetic carbon nanofiber for dye adsorption by hydrothermal treatment coupled with carbonization
    (2024-06-01)
    Kaewtrakulchai, Napat
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    Chanpee, Sirayu
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    Pasee, Warit
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    Putta, Ampol
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    Pollution of water resources has recently increased as a result of expanded industrial activity. Recycling waste biomass into bio-adsorbent material offers a cheap, easy, and eco-friendly solution. In this study, magnetic carbon nanofibers (MCNF) with a highly porous structure were developed from magnetite-preloaded horse manure by hydrothermal treatment followed by carbonization using different ratios of iron (III) nitrate and iron oxide as magnetic precursors. The produced MCNF had a very porous structure with specific surface area of 435.31 m<sup>2</sup>/g and high carbon content. The magnetic characteristics of MCNF promoted by the presence of iron oxide species. The saturated magnetization of MCNF obtained from a 5:5 ratio of the magnetic precursors (iron (III) nitrate: iron oxide) was 2.48 emu/g. Synthesized MCNF was applied as a bio-adsorbent for methylene blue (MB) removal from aqueous solution, with results showing excellent dye adsorption of 92–99 %. MB adsorption was facilitated by pore filling, electrostatic contact, hydrogen bonding, and ion complexation. Experimental results indicated that the Freundlich isotherm and pseudo-second-order kinetic models concurred with the observed MB adsorption data, suggesting that the adsorption mechanism involved multilayered micropore interactions between magnetite and MB chemisorption. The resulting magnetic adsorbent was successfully removed from the aqueous solution by physical separation. Findings indicated that horse manure-derived MCNF could be used as an efficient bio-adsorbent to remove organic contaminants in wastewater.
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    Enhancements of growth and metabolites of indica rice callus (Oryza sativa l. cv. pathumthani1) using tio2 nanoparticles (nano-tio2)
    (2020-04-01) ;
    Sutjaritvorakul, T.
    In this research, the effects of a various in concentration of TiO<inf>2</inf> nanoparticles (Nano-TiO<inf>2</inf>) were applied to study the cell growth, metabolites and antioxidant responses of callus in indica rice plants. Dose-dependent changes in growth performances, metabolite accumulations and antioxidant activities of callus were found. Mature seeds of indica rice cv. Pathumthani1 were cultured in callus induction medium with different concentrations of Nano-TiO<inf>2</inf> (0-600 mg L<sup>-1</sup>). After five weeks of cultivation, the callus formations were measured in fresh weights and dry weights, and the contents of phenolic compounds, flavonoids, and the antioxidant activities of callus extracts were assayed. The Nano-TiO<inf>2</inf> induced of fresh weights and dry weights in callus formations when compared with the control treatment (0 mg L<sup>-1</sup> Nano-TiO<inf>2</inf>) were also observed. The flavonoid and phenolic compound contents in rice callus exposed to 400 mg L<sup>-1</sup> Nano-TiO<inf>2</inf> were 118% and 139%, respectively, higher than rice callus unexposed to Nano-TiO<inf>2</inf>. Moreover, the antioxidant activities of the callus metabolites studied shows that the percentages of inhibition were up-regulated (84%) when exposed to 400 mg L<sup>-1</sup> Nano-TiO<inf>2</inf>, as a response to the high-level depletion of the free radicals in metabolites of rice callus. The results obtained from this research showed that the addition of Nano-TiO<inf>2</inf> had impacts on the callus growth and metabolite accumulations in rice callus. The results suggested that addition of Nano-TiO<inf>2</inf> at appropriate levels could role as an elicitor for biosynthesis of valuable metabolites and antioxidant properties for pharmaceutical applications in further research.
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    Synthesis of porous carbon materials from water hyacinth via hydrothermal carbonization assisted chemical activation for carbon-based electrode applications
    (2020-10-26)
    Liamprawat, Tanatorn
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    Verasarut, Panupong
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    Kaewtrakulchai, Napat
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    Panomsuwan, Gasidit
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    Recently, lignocellulosic materials have been widely utilized as feedstocks for several applications such as carbon, biofuels and biochemical productions because of their potentials (i.e. waste reduction, carbon sequestration, renewable). In this study, porous carbon was successfully synthesized from water hyacinth (WHs) via hydrothermal carbonization assisted with chemical activation using Na2CO3 and K2CO3. The hydrothermal carbonization process was studied in the range of 160-200°C for 4-12h and the hydrothermal chars were then further pyrolyzed under a supply of N2 flow 100ml/min at 700-900°C for 2h. The as-pyrolyzed chars were then activated by two different bases including Na2CO3 and K2CO3 at the ratio of 1.0 (w/w, hydrothermal char: chemical) to obtain highly porous carbon. The results indicated that carbon percentage, surface area and porous structure were improved with the higher hydrothermal temperature showing the best results at 180°C for 8h. Moreover, the development of pore structure of WHs porous carbon was successfully by chemical activation with K2CO3.
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    Differential responses to nanoparticles induced by nanocarbon application in pigmented rice seedlings
    (2020-10-26)
    Samart, S.
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    To investigate the effects of nanocarbon on physiological responses of plant growth, contents of photosynthetic pigments, and the activities of antioxidant enzymes in the seedlings of pigmented rice cv. Hom-nin that were cultured on either untreated-nanocarbon (control) or treated-nanocarbon (soil:nanocarbon, 8:1, 6:1, 2:1 w/w) were studied. The results showed that growth performances, photosynthetic pigment contents and antioxidant enzyme activities in seedlings 7-28 days after the treated-nanocarbon were significantly differenced when compared to the control treatment. Growth performances, photosynthetic pigment contents and antioxidant enzyme activities in pigmented rice seedlings were significantly enhanced by the low and moderate concentrations of nanocarbon treatments (soil:nanocarbon, 8:1, 6:1 w/w) for all sampling dates (7, 14, 21 and 28 days after the nanocarbon treatments). While at the high concentration of nanocarbon (soil:nanocarbon, 2:1 w/w) treatment, growth performances, photosynthetic pigment contents and antioxidant enzyme activities in pigmented rice seedlings were significantly decreased when compared to the other treatments. The results suggested that the inducible physiological responses in pigmented rice seedlings to nanocarbon depend on both the concentrations and the exposure duration. The appropriate ratio of 8:1 and 6:1 w/w of soil:nanocarbon seemed to induce the physiological performances in the seedlings, more than those at the high concentration and untreated of nanocarbon.
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    Titanium dioxide (TiO2) nanoparticles induced callus induction and plant regeneration of indica rice cultivars (suphanburi1 and suphanburi90)
    (2018-10-01) ;
    Sutjaritvorakul, T.
    Efficient plant regeneration systems are useful for indica rice using micropropagation and/or genetic transformation. Nanomaterials provide applications and opportunities in various fields of life science and agriculture technology. In this research, indica rice cultivars ‘Suphanburi1’ and ‘Suphanburi90’ (Oryza sativa L. ssp. indica) were used to investigate. Seeds were examined to evaluate callus induction and plant regeneration frequency. Culture of sterilized-seeds on callus induction medium supplemented with 50 mg L<sup>-1</sup> of TiO<inf>2</inf> nanoparticles induced a high frequency of callus induction and content of biomass accumulation. Embryogenic calli were transferred to plant regeneration medium supplemented with 10-50 mg L<sup>-1</sup> TiO<inf>2</inf> nanoparticles. The highest frequency of plant regeneration and the ratio of the number of seedlings to the number of regenerated calli were produced on plant regeneration medium supplemented with 40 mg L<sup>-1</sup> of TiO<inf>2</inf> nanoparticles. The regenerated shoots induced roots on NB medium without plant growth regulators and the plantlets were established in soil. The results may be used for nanomaterial applications of rice and/or other plants for its further improvement.
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    Highly porous carbon materials for adsorbent from water hyacinth via hydrothermal carbonization
    (2020-10-26)
    Chanpee, Sirayu
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    Suksai, Nattaya
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    Kaewtrakulchai, Napat
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    Fuji, Masayoshi
    Carbon nanoparticles have been successfully synthesized from waste lignin via carbonization and base activation. The effect of base activation on lignin transformation to carbon nanoparticles was investigated. Carbon nanoparticles from waste lignin via carbonization and base activation were denoted as CNPs. The physicochemical properties of CNPs were comprehensively characterized through a Scanning electron microscope (SEM), X-ray diffraction (XRD), and Raman spectroscopy. The results revealed that the condition using 5 wt% of sodium hydroxide (NaOH) and carbonization at 700°C on N2 atmosphere exhibited the highly porous structure of carbon nanoparticles. In conclusion, the NaOH-treated CNPs led to the production of high specific surface areas and high micropore volumes.
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    Nanocarbon induced modifications in morpho-physiopgical characteristics in rice plants [oryza sativa l. cv. black jasmine rice (Hom-nin)]
    (2020-01-01)
    Samart, Sutichai
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    The aim of this research was to evaluate the potential application of nanocarbon in plant growth and physiological responses in rice plants. This research was designed to evaluate the effects of different concentration of nanocarbon (0-1,000 mg L<sup>-1</sup>) on growth characterizations and physiological parameters after 1-4 weeks of nanocarbon-treatment. Induction in the growth performances and contents of photosynthetic pigments (chlorophyll A, chlorophyll B and carotenoids), antioxidant enzyme activities (catalase and peroxidase) and flavonoids was observed in the rice plants after treated with nanocarbon. The results showed that nanocarbon promoted the plant growth in rice plants cv. Black jasmine rice or Hom-nin by increasing the shoot lengths and dry weights. The significant increase of shoot length and dry weight were found in 200 and 600 mg L<sup>-1</sup> nanocarbontreatments. Additionally, the high contents of photosynthetic pigment concentrations in treated-plants showed significant difference at 600 mg L<sup>-1</sup> nanocarbon compared to the control (0 mg L<sup>-1</sup> nanocarbon). The antioxidant activities of catalase and peroxidase enzymes in nanocarbon treated-plants were significantly increased compared to the control. Furthermore, the induction of flavonoid contents in treated-plants was increased by 200 and 600 mg L<sup>-1</sup> nanocarbon. The results suggested that nanocarbon able to promote the biomass and has benefit to increase the photosynthetic pigments and antioxidant enzymes for plant growth performances. This research provided plant growth and physiological responses evidence on the beneficial effect of nanocarbon in rice plants, especially Black jasmine rice or Hom-nin cultivar.