KMITL

Permanent URI for this communityhttps://dspace.kmitl.ac.th/handle/123456789/1

Browse

Search Results

Now showing 1 - 6 of 6

Some of the metrics are blocked by your
consent settings
In silico encrypted peptide from green alga Tetraspora sp. CU2551 showed high antimicrobial activities
(2024-03-01)
Tonawut, Yothawut
;
Rittiyan, Nutnicha
;
Kornrawudaphikasama, Yosita
;
Klinsalee, Rachaneekorn
;
Laokua, Natwikar
The green alga Tetraspora sp. CU2551 has been previously identified as a biohydrogen producer for an alternative option for sustainable energy production development. Its biomass still contains a source of valuable biomolecules, especially proteins. Crude algal protein showed significant antimicrobial activity against Gram-positive bacteria (Bacillus subtilis TISTR 1248 and Staphylococcus aureus TISTR 746) and Gram-negative bacteria (Escherichia coli TISTR 074 and Pseudomonas aeruginosa TISTR 2370), representing a clear zone of 19.0 ± 0.1, 18.3 ± 0.5, 17.7 ± 0.5, and 19.0 ± 0.5, respectively. Further investigation revealed that encrypted peptides from pepsin-hydrolyzed crude proteins increased the inhibition activity against these bacteria by 3.4–10.5 %. The hydrolyzed peptides were fractionated using a Sephadex G-25 column, and peptides in fraction 17 showed the highest antimicrobial activity. Through peptide identification using LC-MS and prediction of obtained peptide primary sequences with four bioinformatic tools (DBAASP, AMPDiscover, iAMPpred, and AMPscanner), five peptides were selected and synthesized for activity evaluation. The PT11 peptide (RYGAAMAVGIACA) demonstrated the highest antimicrobial activity among the five peptides tested and compared favorably to previously reported algal encrypted peptides. The findings of this research demonstrate that the encrypted PT11 peptide derived from Tetraspora sp. CU2551 has promising potential as an antimicrobial agent. Additionally, we suggest that PT11 might be used for other things besides this, like animal feed.
Some of the metrics are blocked by your
consent settings
Identification of major carotenoids from green alga Tetraspora sp. CU2551: partial purification and characterization of lutein, canthaxanthin, neochrome, and β-carotene
(2022-08-01)
Maswanna, Thanaporn
;
Maneeruttanarungroj, Cherdsak
The green algae Tetraspora sp. CU2551 was previously identified as a strain with high potential for biohydrogen production; however, its algal biomass characteristics changed from green to reddish orange within 43 days of biohydrogen production. The crude pigments were extracted, partially purified, and characterized by chemical determination. The present study focused on elucidating the carotenoid composition of the selected green alga Tetraspora sp. CU2551. The pigment extract was partially purified and fractionated using thin layer chromatography, and yielded two major and two minor carotenoid bands. The fractions were confirmed by high-performance liquid chromatography with a diode array detector (HPLC–DAD) before being identified and confirmed using Liquid Chromatograph-Quadrupole Time of Flight-Mass Spectrometry (LC-QTOF-MS). The spectral data of these fractions revealed four sub-fractions of interest that were lutein, canthaxanthin, neochrome, and β-carotene, which had percentages in the crude extracts of 30.57%, 25.47%, 7.89%, and 0.71%, respectively. Lutein and canthaxanthin were found to be the major carotenoid pigments present. Our findings in this present study are the first reporting of Tetraspora sp. CU2551 as a potential alternate source for carotenoid pigment production.
Some of the metrics are blocked by your
consent settings
Response of green alga Tetraspora sp. CU2551 under potassium deprivation: a new promising strategy for hydrogen production
(2022-04-01)
Pewnual, Totsapon
;
Jampapetch, Narirat
;
Saladtook, Sathonkorn
;
Raksajit, Wuttinun
;
Klinsalee, Rachaneekorn
With increasing world energy consumption, renewable energy sources can fulfill the need and many have net zero carbon dioxide emissions. One of these is hydrogen, which is biologically produced molecular hydrogen from organisms. Although many studies have produced hydrogen from green algae and optimized conditions to obtain the highest yield, following our previous works on production optimization from Tetraspora sp. CU2551, we tested deprivation of Fe, Cu, Ca, S, Mg, or K, and surprisingly found that K deprivation showed another promising switch in increased H<inf>2</inf> yield, representing 9.2 ± 0.1 μmol mg−1 DW within 32 h incubation anaerobically. Moreover, longer cell adaptation by aerobic incubation, before production phase, resulted in higher hydrogen yield. Cells adapted in potassium deprivation (TAP-K medium) promoted hydrogen production to a yield of 14.8 ± 0.03 μmol mg−1 DW, or about 3.0 times higher than normal TAP (5.0 ± 0.82 μmol mg−1 DW). The increased yield was caused by (1) lowering PSII activity (direct biophotolysis), resulted in less oxygen being produced, lowering hydrogenase inhibitor levels, and (2) increasing accumulated starch degradation (indirect biophotolysis) (12.2% starch remaining or 7.2 ± 0.9 μg mg−1 DW), increased the flow of electrons to hydrogenase. Our finding makes potassium deprivation conditions as another the promising choice to enhance hydrogen production in biological systems.
Some of the metrics are blocked by your
consent settings
Improved biohydrogen production by immobilized cells of the green alga Tetraspora sp. CU2551 incubated under aerobic condition
(2020-10-01)
Maswanna, Thanaporn
;
Lindblad, Peter
;
Maneeruttanarungroj, Cherdsak
The green alga Tetraspora sp. CU2551 was previously investigated and showed enhanced H<inf>2</inf> production under anaerobic (Ar purged) conditions by cells immobilized in a calcium alginate matrix (Maswanna et al., Biomass Bioenergy 111:88–95, 2018). Here, we report successful H<inf>2</inf> production in entrapped cells under aerobic conditions. The most favorable immobilization condition observed was 4% (w/v) final alginate concentration after gelation, 2.80- to 3.35-mm beads, and a biomass content of 0.125 mg DW mL−1 alginate. H<inf>2</inf> production increased when the immobilized cells were incubated in S-deprived media which could be repeated up to six times when using refreshed media. After six cycles, the H<inf>2</inf> production reached 12.8 ± 0.9 mL H<inf>2</inf> 25 mL−1 of medium, corresponding to a rate of 182 ± 20 nmol mg−1 DW h−1, which was significantly higher than previously observed for other microalgae. Thus, our results demonstrate a potential for photobiological H<inf>2</inf> production using immobilized Tetraspora sp. CU2551 cells, grown under ambient aerobic conditions.
Some of the metrics are blocked by your
consent settings
Enhanced hydrogen production by optimization of immobilized cells of the green alga Tetraspora sp. CU2551 grown under anaerobic condition
(2018-04-01)
Maswanna, Thanaporn
;
Phunpruch, Saranya
;
Lindblad, Peter
;
Maneeruttanarungroj, Cherdsak
The green alga Tetraspora sp. CU2551 has previously been identified and characterized as a photosynthetic microorganism with high potential for H<inf>2</inf> production. In the present study, cells of Tetraspora CU2551 were entrapped and immobilized in an alginate matrix with the aim to analyze the effect of cell stacking and a reduced exposure of O<inf>2</inf> to the cells. The results showed that the most favorable immobilization conditions were 4% (w/v) of final alginate concentration and a cell concentration of 0.125 mg cell dry wt/mL alginate with a bead diameter of 2.80–3.35 mm. The H<inf>2</inf> production yields increased when the immobilized cells were incubated in S-deprived medium and this could be repeated at least for 3 production times. Maximal total H<inf>2</inf> production reached 7.68 ± 0.88 mL H<inf>2</inf>/25 mL medium, corresponding to a rate of 1182.45 ± 24.40 nmol H<inf>2</inf>/h/mg DW. This production is about 6 times higher compared to by cells in suspension, 2–10 times higher when compared to by other green algae, and 10–50 times higher when comparing with cyanobacteria. Based on our observations, immobilized cells of Tetraspora CU2551 is considered a very promising biological system for significant photobiological H<inf>2</inf> production by a photosynthetic microorganism.
Some of the metrics are blocked by your
consent settings
Effect of pH on Biohydrogen Production in Green Alga Tetraspora sp. CU2551
(2017-01-01)
Maneeruttanarungroj, Cherdsak
;
Phunpruch, Saranya
The green alga Tetraspora sp. CU2551 has previously been identified as a hydrogen producing organism. With the promising potential in a high H<inf>2</inf> production yield, we further studied the enhancement of the H<inf>2</inf> production capacity by a substrate-level control to the key enzyme, Hydrogenase. This enzyme catalyzes the formation of biohydrogen gas from protons and electrons. Here, we investigated the effect of pH on the production rate which directly affected the flow of proton flux to the hydrogenase enzyme. The result revealed that pH of culture TAP medium was simply controlled by the use of HCl/NaOH solution over the pH range of 6.5 - 9.0 without any effect on the growth of algal cells. The H<inf>2</inf> production was observed in all ranges of our focusing pH with comparable to the control condition (pH 7.0 of TAP medium). In our best condition, an increase of the H<inf>2</inf> yield about 24% was found in cells cultivated in TAP medium adjusted the pH to 6.5 compared to the control condition. Our finding confirms the successful biohydrogen production enhancement by a substrate-level control for the key hydrogenase enzyme.

KMITL

Browse

Filters

Settings

Sort By

Results per page

Search Results