Phunpruch, Saranya

Preferred name

Phunpruch, Saranya

Alternative Name

Phunpruch, S.

Main Affiliation

School of Science

saranya.ph@kmitl.ac.th

Now showing 1 - 10 of 55

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Biomass Production of Chlorella vulgaris var. vulgaris TISTR 8261 During Cultivation in Modified Food Industry Wastewater
(2025-12-01)
Taikhao, Samart
;
Phunpruch, Saranya
Industrial wastewater can serve as a low-cost nutritional source for sustainable microalgal biomass production. This study investigated the biomass of Chlorella vulgaris var. vulgaris TISTR 8261 grown in untreated wastewater collected from four food industry factories in Phra Nakhon Sri Ayutthaya Province, Thailand. Among them, wastewater from a processed food production plant (PFPP) supported the highest algal growth. Supplementation with 17.4 mM sodium acetate significantly improved algal biomass yield. Further optimization with 3.7 mM NH<inf>4</inf>Cl, 1.0 mM KH<inf>2</inf>PO<inf>4</inf>, 0.2 mM MgSO<inf>4</inf>, and a moderate concentration of trace minerals enhanced the specific growth rate and chlorophyll concentration. Scaled-up cultivation in 3.5 L culture bottles in optimized PFPP yielded a maximum biomass yield of 8.436 ± 0.378 g L−1, comparable to 6.498 ± 0.436 g L−1 in standard TAP medium. Biomass composition analysis after 15 days of cultivation revealed 42.70 ± 1.40% protein, 17.10 ± 1.60% carbohydrate, and 1.90 ± 0.10% lipid on a dry weight basis. These findings demonstrate that optimized PFPP wastewater can effectively support high-density cultivation of C. vulgaris var. vulgaris TISTR 8261, yielding nutritionally rich biomass, and offering a cost-effective and environmentally sustainable strategy for industrial-scale microalgal production.
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Physiological and Environmental Factors Influencing Hydrogen Production by Unicellular Green Alga Monoraphidium sp. KMITL-1
(2025-10-01)
Krutpan, Varanya
;
Supakriangkrai, Thaninthorn
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Lohasupthawee, Pana
;
Phunpruch, Saranya
With the growing global energy demand and the urgent need to reduce carbon emissions, hydrogen (H<inf>2</inf>) has emerged as a promising clean energy carrier. Among various biological H<inf>2</inf> production, green algae present a sustainable and eco-friendly alternative due to their ability to produce H<inf>2</inf> via photobiological pathways. This study aimed to investigate H<inf>2</inf> production by unicellular green alga Monoraphidium sp. KMITL-1, isolated from hydroponic water at the Plant Tissue Culture Laboratory, King Mongkut’s Institute of Technology Ladkrabang. The taxonomic identity of the strain, belonging to the genus Monoraphidium within the Selenastraceae family, was confirmed through morphological observation and molecular characterization using 23S plastid rRNA gene sequencing. Various physiological and environmental parameters influencing H<inf>2</inf> production were evaluated, including cell age, cell density, nutrient deprivation, carbon source, pH, temperature, and light intensity. A 24-hour-old culture with an OD<inf>750</inf> of 0.8 exhibited a significant increase in H<inf>2</inf> production. The optimal medium was potassium-deprived Tris-acetate-phosphate (TAP-K) supplemented with glucose at a concentration of 350 mmol C-atom L-1. The ideal environmental conditions for H<inf>₂</inf> production were pH 7.2, a temperature of 30 °C, and a light intensity of 60 μmol photons m-2 s-1. Under these optimized conditions, Monoraphidium sp. KMITL-1 achieved a maximum H<inf>2</inf> production rate of 67.976 ± 1.096 μmol H<inf>2</inf> mg Chl-1 h-1 and a cumulative H<inf>2</inf> yield of 3,190.436 ± 2.219 μmol H<inf>2</inf> mg Chl-1 after 72 h of incubation. These results highlight the potential of Monoraphidium sp. KMITL-1 for large-scale biohydrogen production and its applicability in the development of sustainable energy technologies.
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Biomass and biohydrogen production by unicellular green alga Chlorella vulgaris var. vulgaris TISTR 8261 using frozen food industrial wastewater
(2022-01-01)
Taikhao, Samart
;
Phunpruch, Saranya
Biohydrogen production by green algal biomass is promising method for sustainable H<inf>2</inf> production and bioenergy recovery. In this approach, green algae convert organic and inorganic substances (used as the sole source of carbon and electrons) in wastewater into H<inf>2</inf>. In this study, biomass and H<inf>2</inf> production from the unicellular green alga Chlorella vulgaris var. vulgaris TISTR 8261, cultivated in frozen food industrial wastewater, was investigated. The results revealed that growth rate of algal cells cultivated in treated wastewater was significantly higher than that in untreated wastewater but lower than that in the synthetic control Tris acetate phosphate (TAP) medium. In addition, the cells grown in treated wastewater exhibited a high ability to remove nitrate, nitrite, phosphate, and sulfate from the water. Furthermore, algal cells were cultured with various concentrations of sodium acetate (0-17.4 mM); the optical density of the cultures at 750 nm increased with increase in acetate concentration. Cell growth in treated wastewater supplemented with 17.4 mM sodium acetate was similar to that in TAP medium. The highest H<inf>2</inf> production of 12.87 ± 0.58 µmolH<inf>2</inf> mg Chl a-1 was observed in cells incubated in treated wastewater supplemented with 17.4 mM sodium acetate; this yield was higher than that obtained from cells incubated in nitrogen-free TAP medium. The results of this study support the potential use of wastewater for biomass and biohydrogen production by C. vulgaris var. vulgaris TISTR 8261.
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Simazine Enhances Dark Fermentative H2 Production by Unicellular Halotolerant Cyanobacterium Aphanothece halophytica
(2022-07-15)
Pansook, Sunisa
;
Incharoensakdi, Aran
;
Phunpruch, Saranya
The halotolerant cyanobacterium Aphanothece halophytica is a potential H<inf>2</inf> producer that induces H<inf>2</inf> evolution under nitrogen deprivation. H<inf>2</inf> is mainly produced via the catabolism of stored glycogen under dark anaerobic condition. H<inf>2</inf> evolution is catalyzed by O<inf>2</inf>-sensitive bidirectional hydrogenase. The aim of this study was to improve H<inf>2</inf> production by A. halophytica using various kinds of inhibitors. Among all types of inhibitors, simazine efficiently promoted the highest H<inf>2</inf> production under dark conditions. High simazine concentration and long-term incubation resulted in a decrease in cell and chlorophyll concentrations. The optimal simazine concentration for H<inf>2</inf> production by A. halophytica was 25 µM. Simazine inhibited photosynthetic O<inf>2</inf> evolution but promoted dark respiration, resulting in a decrease in O<inf>2</inf> level. Hence, the bidirectional hydrogenase activity and H<inf>2</inf> production was increased. A. halophytica showed the highest H<inf>2</inf> production rate at 58.88 ± 0.22 µmol H<inf>2</inf> g−1 dry weight h−1 and H<inf>2</inf> accumulation at 356.21 ± 6.04 μmol H<inf>2</inf> g−1 dry weight after treatment with 25 µM simazine under dark anaerobic condition for 2 and 24 h, respectively. This study demonstrates the potential of simazine for the enhancement of dark fermentative H<inf>2</inf> production by A. halophytica.
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A role for 16S rRNA dimethyltransferase (ksgA) in intrinsic clarithromycin resistance in Mycobacterium tuberculosis
(2013-06-01)
Phunpruch, Saranya
;
Warit, Saradee
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Suksamran, Rungaroon
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Billamas, Pamaree
;
Jaitrong, Sarinya
The emergence of extensively drug-resistant tuberculosis (XDR-TB) makes the control of tuberculosis (TB) difficult. As a result, there is an urgent need to develop new anti-TB drugs. Alternatively, drugs that have already been used in humans as anti-infectives and later found to have antitubercular activity might be useful as anti-TB drugs, particularly against drug-resistant TB. Clarithromycin (CLR), a 14-membered macrolide and protein synthesis inhibitor, has potent activity against most mycobacterial infections, except Mycobacterium tuberculosis. Mycobacterium tuberculosis is naturally resistant to CLR [minimum inhibitory concentration (MIC) of 8-16 μg/mL] owing to the presence of inducible erm methylase (ErmMT). With a view to gaining further insight into the mechanisms of innate CLR resistance in M. tuberculosis, CLR-susceptible M. tuberculosis H37Rv mutants were generated by transposon mutagenesis. One mutant, designated as Tn-196, was further investigated and it was found that ksgA (Rv1010) was inactivated by the transposon. The ksgA gene encodes a 16S rRNA adenine dimethyltransferase that methylates A1518 and A1519 (Escherichia coli numbering) of 16S rRNA and plays an important role in ribosome biogenesis. Complementation of the Tn-196 mutant with a wild-type ksgA gene restored the resistant phenotype (MIC of 8-16 μg/mL), corroborating the association of ksgA with intrinsic CLR resistance in M. tuberculosis. © 2013 Elsevier B.V. and the International Society of Chemotherapy.
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Cellular lipid production of a heterotrophic bacterium isolated from poultry processing wastewater
(2014-01-01)
Ongmali, Rattiya
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Phunpruch, Saranya
;
Thawornchaisit, Usarat
Cell growth and lipid production of a heterotrophic bacterium, R4.4 which accumulated the highest lipid content among 12 bacterial colonies being isolated from wastewater of a poultry processing plant in Thailand, were evaluated in this study. The culture was identified as Aeromonas sp. by 16S ribosomal DNA sequencing analysis.The highest lipid content was obtained when the cells were in the early stationary growth phase compared to the cells in the exponential and the late stationary phase. Over 50% of the fatty acid production by Aeromonas sp. KMITL-R4.4 were unsaturated fatty acids, including linoleic acid (C18:2, 43.2%), oleic acid (C18:1, 19.0%), and palmitoleic acid (C16:1, 8.2%). The culture had a preference for glucose and fructose as seen from the maximum biomass and lipid contents that were obtained. Among volatile fatty acid (VFA) species tested, acetic acid was the preferred substrate for lipid production but not favorable for cell growth. In addition, ammonium sulfate was found to be the best among nitrogen sources tested. The C/N ratio exerts significant impact on lipid production as seen from an increase of the lipid content from 10.8% to 18.2% by exposing the bacterial cells to a medium with lower nitrogen concentration (0.1g/l) and higher level of glucose (28 g/l).
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Screening and identification of lactic acid bacteria from raw seafoods and thai fermented seafood products for their potential use as starter cultures
(2012-01-01)
Nanasombat, Suree
;
Phunpruch, Saranya
;
Jaichalad, Thitirut
The number of lactic acid bacteria (LAB) was analyzed from 52 samples of raw seafoods (shrimp and mussel), and Thai fermented seafood products including fermented shrimp (kung-jom), mussel (hoi-dong), and fish (pla-jom). The viable LAB were 3.0×103 to 3.4×108 CFU/g. LAB were isolated and screened for their inhibitory activities against eight indicator bacteria by agar spot test. Among all selected LAB isolates, 52 isolates showed strong inhibitory activity. They were further characterized for their ability to resist hydrochloric acid, lactic acid, bile salts, and sodium chloride, and their ability to produce bacteriocins and amino acid decarboxylase. The selected LAB isolates, 1IS11 and 4IS17, were bacteriocin-producing strains, and showed no amino acid decarboxylase activity, which was suitable property for starter cultures. The isolate 1IS11 could resist both hydrochloric and lactic acid at the lowest pH of 2.0, while the isolate 4IS17 was able to tolerate hydrochloric and lactic acid at the lowest pH of 1.5 and 2.0, respectively. Both isolates could grow in MRS broth containing a high concentration of sodium chloride (10 %) and bile salts (1.5%). They were identified by morphological characterization, biochemical test, and 16S rDNA sequence analysis. The isolate 1IS11 was found to be Enterococcus faecium, whereas the isolate 4IS17 was Enterococcus faecalis.
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Free radical scavenging properties and induction of apoptotic effects of fa fraction obtained after proteolysis of bioactive peptides from microalgae synechococcus sp. VDW
(2019-01-01)
Suttisuwan, Rutairat
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Phunpruch, Saranya
;
Saisavoey, Tanatorn
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Sangtanoo, Papassara
;
Thongchul, Nuttha
This study aims to determine the antioxidant activity of bioactive peptides derived from Synechococcus sp. VDW cells cultured for 21 days. Synechococcus sp. VDW protein hydrolysates were prepared with trypsin and purified by ultrafiltration with molecular mass cut-off membranes of 10, 5 and 3 kDa. The M<3 kDa (FA) fraction had the highest 2,2'-azino- bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) and 2,2'-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activities, with IC50 values of (11.5±0.3) and (13.6±0.2) μg/ mL, respectively. The FA fraction was separated by reversed phase HPLC to yield four subfractions (F1-4). The F4 subfraction showed the highest maximum ABTS radical scavenging activity (3.55±0.61) % and it was selected for further analysis by electrospray ionisation quadrupole time-of-flight mass spectrometry (ESI-Q-TOF-MS/MS) based on de novo peptide sequencing. Five antioxidant peptides were identified, of which AILESYSAGKTK had the highest ABTS radical scavenging activity. Furthermore, the FA fraction showed high cytotoxic activities against human cancer-derived cell lines, especially the colon cancer cell line (SW620) with an IC50 value of (106.6±21.5) μg/mL, but not the untransformed Wi38 cell line. The FA fraction activated the apoptotic pathway in SW620 cells after treatment for 24, 48 and 72 h, with the highest activities of caspases-3, -8 and -9 being observed after treatment for 72 h. These findings suggested that microalgae Synechococcus sp. VDW may be used to develop natural anticancer drugs.
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Isolation and characterization of anti-inflammatory peptides derived from trypsin hydrolysis of microalgae protein (Synechococcus sp. VDW)
(2019-10-02)
Suttisuwan, Rutairat
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Phunpruch, Saranya
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Saisavoey, Tanatorn
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Sangtanoo, Papassara
;
Thongchul, Nuttha
The Synechococcus sp. VDW peptides were prepared by trypsin digestion, which was then purified by ultrafiltration with molecular weight cut off membranes of 10, 5 and 3 kDa. Among the fractions, the MW <3 kDa fraction exhibited high levels of inhibitory activity toward the NO radical scavenging activities with IC<inf>50</inf> values of 34.51 ± 9.8 μg/mL. The MW <3 kDa fraction was purified by reversed-phase HPLC to yield four fractions. The 30–40 min sub-fraction with maximum NO radical scavenging activity (77.50 ± 0.55%) was selected for further analysis by Q-TOF ESI mass spectrometry. Five isolated peptides with amino acid sequences of AILQSYSAGKTK; 1,265.69 Da, ALNKTHLIQTK; 1,265.74 Da, LLVHAPVK; 875.55 Da, IPDAHPVK; 875.48 Da, and VVVLRDGAVQQLGTPR; 1,706.97 Da were identified. The MW <3 kDa fraction showed no cytotoxicity toward RAW264.7 macrophage cells. Quantitative RT-PCR results showed that the MW <3 kDa fraction reduced gene expression of pro-inflammatory cytokines iNOS, TNF-α, COX-2, and IL-6. The peptides isolated from the MW <3 kDa fractions represent potential model peptides to develop natural anti-inflammation food-grade ingredients, drugs, and cosmetic products.
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Control of Aspergillus postharvest rot on plum tomato by potassium metabisulfite and potassium sorbate
(2025-01-01)
Nanasombat, Suree
;
Phunpruch, Saranya
In this study, fungal contamination of plum tomato fruits (Solanum lycopersicum) was preliminarily examined by the dilution plating method. The tomato samples were highly contaminated by molds and yeasts (1.0x105-1.4x108 CFU/g), including Aspergillus, Geotrichum, Penicillium, Rhizopus, Candida, Cryptococcus and Rhodotorula. The mold strains that contaminated the tomatoes to the greatest extent were Penicillium and Rhizopus. Three fungal isolates (Aspergillus niger T6D5, Candida guilliermondii T7D4 and Rhodotorula mucilaginosa T7D5) were selected for further in vitro study. Then, the antifungal activity of organic acids (citric and tartaric acids) and salts (potassium acetate, potassium metabisulfite and potassium sorbate) against molds and yeasts was investigated. Salt solutions showed higher inhibitory effects than did acid solutions. Interestingly, A. niger T6D5 showed the highest susceptibility to potassium sorbate and potassium metabisulfite at 0.01-1% (w/v) MIC, respectively, compared to other fungi. In yeasts, potassium metabisulfite effectively inhibited the growth of C. guilliermondii T7D4 and R. mucilaginosa T7D5 at 0.25% (w/v) MIC. The use of organic acids and salts to control the growth of A. niger T6D5 on tomatoes was investigated. Among all the salts and acids tested, 1% (w/v) potassium metabisulfite and 3% (w/v) potassium sorbate completely inhibited the growth of A. niger T6D5 on tomatoes.

Phunpruch, Saranya

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