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Author: search.filters.author.Ali, Ali Muhammed MoulaSubject: search.filters.subject.Characterization

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    Characterization of acid soluble collagen from the skin of snakeskin gourami (Trichogaster Pectoralis)
    (2020-01-01)
    Sukkon, Piyaporn
    ;
    Ali, Ali Muhammed Moula
    ;
    Nalinanon, Sitthipong
    ;
    Kishimura, Hideki
    ;
    Takeungwongtrakul, Sirima
    The present study was aimed to isolate and characterized acid soluble collagen (ASC) from the skin of snakeskin gourami (Trichogaster pectoralis). ASC from gourami skin had a yield of 9.43% and 34.65%, based on wet and dry weight basis, respectively. The purity of ASC was superior with a distinct absorption peak at wavelength (WL) of 230.7 nm. Based on the electrophoretic pattern, gourami skin ASC was classified as type I collagen, as it comprised α1 and α2 as major components and higher molecular weight (MW) components γ, β were distinctly observed. ASC exhibited high Tmax value of 33.43°C, which could correspond to its imino acids content of 188 residues/1000 residues. Fourier transform infrared (FTIR) spectrum and circular dichroism (CD) revealed that ASC extracted from gourami skin had greater structural integrity in its triple-helical form. Solubility of ASC was high at the pH range of 2-4 in which zeta potential exhibited highly positive charge. The highest solubility of ASC in the presence of NaCl was observed at 2% (w/v). Therefore, with all the characteristic features, ASC from snakeskin gourami skin can be a valueadded product in the fish processing industry.