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Author: search.filters.author.Chamroensaksri, NitchaSubject: search.filters.subject.Glycomyces amatae

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    Item type:Publication,
    Glycomyces amatae sp. nov., isolated from a yellow-ringed grass moth (Amata sperbius)
    (2022-01-01)
    Suriyachadkun, Chanwit
    ;
    Ngaemthao, Wipaporn
    ;
    Pujchakarn, Tawanmol
    ;
    Chamroensaksri, Nitcha
    ;
    Niemhom, Nantawan
    A novel mycelium-forming actinomycete strain, designated A-F 0318<sup>T</sup>, was isolated from a yellow-ringed grass moth (Amata sperbius) collected from Phitsanulok Province, Thailand. Long chains of non-motile cylindrical spores with a smooth surface developed on aerial mycelia. The polyphasic taxonomic study suggested that strain A-F 0318<sup>T</sup> belonged to the genus Glyco-myces. The 16S rRNA gene sequence analysis indicated that strain A-F 0318<sup>T</sup> was closely related to Glycomyces harbinensis LL-DO5139<sup>T</sup> with 97.94 % sequence similarity. The average nucleotide identity (ANI) based on blast, ANI based on the MUMmer algorithm and average amino acid identity values of strain A-F 0318<sup>T</sup> with G. harbinensis LL-DO5139<sup>T</sup> were 86.9, 89.1 and 84.24 %, respectively. The digital DNA–DNA hybridization value between A-F 0318<sup>T</sup> and its closest relative, G. harbinensis LL-DO5139<sup>T</sup> was 33.8 %. The digital G+C content of the genomic DNA was 71.7 mol%. The cell-wall peptidoglycan contained meso-diaminopimelic acid. The whole-cell sugars contained ribose, xylose, glucose and galactose. The predominant menaquinone was MK-10(H<inf>4</inf> ). The predominant fatty acids were iso-C<inf>16: 0</inf>, anteiso-C<inf>15: 0</inf>, anteiso-C<inf>17: 0</inf> and iso-C<inf>16: 1</inf> G. The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannoside, two unknown phosphoglycolipids and one unknown phospholipid. Based on comparative analysis of genotypic, phenotypic and chemotaxo-nomic data, the novel actinomycete strain A-F 0318<sup>T</sup> (=TBRC 13612<sup>T</sup>=NBRC 115417<sup>T</sup>) represents the type strain of a novel species, for which the name Glycomyces amatae sp. nov. is proposed.