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Author: search.filters.author.Chareonsap, P. P.Subject: search.filters.subject.Shoot induction

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    Plant regeneration of Bauhinia purpurea by tissue culture technique
    (2024-05-01)
    Saparam, W.
    ;
    Poeaim, A.
    ;
    Pongtongkam, P.
    ;
    Chareonsap, P. P.
    ;
    Poeaim, S.
    Results showed that the seed of Bauhinia purpurea germination was archived 100% from half-strength MS medium supplemented with 3.0 mg/L Gibberellic acids (GA3) and gave 71.137 mm for the highest seedling. The optimal condition of nodal explants sterilization was E5, which gave 92% of survival explants. For the shoot induction method, the results showed that 24.270 mm was the highest proliferation of shoots achieved from MS medium supplemented with 1.0 mg/L 6-benzylaminopurine (BAP). The highest number of roots was 2.867 roots, and the highest root length was 33.453 mm, obtained at half-strength MS medium complemented with 0.25 mg/L Indole-3-acetic acid (IAA). The 73.33% of plantlets were survived after being transferred to plastic pot.
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    Micropropagation of Ironwood (Xylia xylocarpa (Roxb.) Taub.) by tissue culture
    (2024-03-01)
    Panudom, S.
    ;
    Pongtongkam, P.
    ;
    Chareonsap, P. P.
    ;
    Poeaim, A.
    ;
    Poeaim, S.
    It was found that the sterilization procedure was highly effective, resulting in a 100% survival rate of Xylia xylocarpa (Roxb.). After sterilization, the seeds were cultured on a half-strength Murashige and Skoog (MS) medium without adding plant growth regulators (PGRs). A maximum germination percentage of 100% was achieved after 2 weeks of culture. 1.5 cm-sized node segments from both seeds and mother trees were used for shoot induction. These node segments were treated with the same sterilization conditions as the seeds but with the addition of 0.1% HgCl2. The node segments achieved a 90% survival rate despite the additional chemicals. The node segments from seeds were then cultured on a full-strength MS medium supplemented with 0.50 mg/l of BAP. The results showed a maximum number of shoots, reaching 4.10±1.20 per explant and a mean shoot length of 3.87±0.30 cm. The part of node segments derived from mother trees showed 3.50±1.27 shoots per explant, accompanied by a mean shoot length of 3.18±0.89 cm when using BAP concentration of 0.25 mg/l after 12 weeks of cultivation. The rooting medium contained half-strength MS medium supplemented with 0.75 mg/l of IBA. After 8 weeks of being cultured in the rooting medium, the shoots exhibited a maximum rooting percentage of about 80%. The technique provides a valuable method for rapidly propagating and conserving this plant species in a controlled and efficient manner.
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    Effect of plant growth regulators on micropropagation of Vanilla aphylla and Vanilla planifolia sp. Variegate
    (2018-12-01)
    Kunwanlop, W.
    ;
    Boonmee, W.
    ;
    Laipasu, P.
    ;
    Chareonsap, P. P.
    ;
    Krajangvuth, T.
    The plant growth regulators for propagating large number of vanillas in a short period of time was reported. Axillary buds were used from the explants of Vanilla aphylla and Vanilla planifolia sp. variegata that cultured on solid Gamborg's B5 medium supplemented with 0, 0.5, 1, 1.5 and 2 mg/L 6-benzylaminopurine (BA) for 8 weeks. The result showed that the highest average number of shoot proliferation and root induction were obtained at 1 mg/L BA of 3.2 cm. and 5.3 cm. respectively for Vanilla aphylla. Vanilla planifolia sp. variegata showed the highest shoot elongation of 1.9 cm. at 0.5 mg/L BA. Root induction was the highest of 0.9 cm. at 2 mg/L BA. After 12 weeks of culture The explants were grew the best after 12 weeks. They were transplanted into pots and covered by plastic bags for acclimatization and has maintained in greenhouse.