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Author: search.filters.author.Charoenporn, C.

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    Antifungal metabolites from antagonistic fungi used to control tomato wilt fungus Fusarium oxysporum f. sp. lycopersici
    (2011-12-28)
    Sibounnavong, P.
    ;
    Charoenporn, C.
    ;
    Kanokmedhakul, S.
    ;
    Soytong, K.
    Chaetomium elatum strain ChE01, Chaetomium lucknowense strain CLT01 and Emericella rugulosa strain ER01, which were isolated from soil in Thailand, effectively controlled the most virulent isolate of Fusarium oxysporum f. sp. lycopersici NKSC02 causing wilt of tomato (Lycopersicon esculentum var Sida). Two antifungal substances were purified: Chaetoglobosin-C from Ch. elatum and Ch. lucknowense and tajixanthone from E. rugulosa. Chaetoglobosin-C showed greater antifungal activity against F. oxysporum f. sp. lycopersici, with an effective dose (ED <inf>50</inf>) of 5.98 μg/ml, compared with tajixanthone (ED <inf>50</inf> of 167 μg/ml). These results suggest that the disease control mechanism of these antagonistic fungi involves antibiosis. The inoculating tomato seedlings var. Sida with conidia of F. oxysporum f.sp. lycopersici, mixed with either a solution of chaetoglobosin-C or tajixanthone showed no wilt symptom. The conidia of F. oxysporum f. sp. lycopersici treated with these two compounds appeared abnormal and lost pathogenicity. © 2011 Academic Journals.
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    Evaluation of bio-agent formulations to control Fusarium wilt of tomato
    (2010-09-06)
    Charoenporn, C.
    ;
    Kanokmedhakul, S.
    ;
    Lin, F. C.
    ;
    Poeaim, S.
    ;
    Soytong, K.
    The ED<inf>50</inf> value of antagonistic substance from Chaetomium globosum N0802 extracted with hexane was 157 μg/ml. This gave the highest inhibition of conidial production of Fusarium oxysporum f. sp. lycopersici causing tomato wilt var Sida. Crude hexane from Chaetomium lucknowense CLT and crude methanol from Trichoderma harzianum PC01 gave ED<inf>50</inf> values of 188 and 192 μg/ml. It clearly demonstrated that antagonistic substances from all tested fungi could be deformed and this could break the conidial cells. The bio-agent formulations namely N0802, CLT and PC01 gave significantly highest disease reduction of tomato wilt which were 44.68, 36.28 and 41.01%, respectively, followed by prochoraz (21.95%). All tested bio-agent formulations could significantly increase the yield of tomato when compared to prochoraz and inoculated control. It is concluded that C. globosum, C. lucknowense and T. harzianum developed as bio-agent formulations namely N0802, CLT and PC01 and showed their abilities to control tomato wilt. © 2010 Academic Journals.