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Author: search.filters.author.Choengchan, NathawutSubject: search.filters.subject.albumin

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    A Novel Cross Injection Analysis for Simultaneous Multi-Determination of Diabetic Nephropathy Biomarkers in Urine
    (2026-05-01)
    Inpota, Prawpan
    ;
    Choengchan, Nathawut
    This work presents, for the first time, a novel cross injection analysis (CIA) system for the simultaneous multi-determination of key biomarkers associated with diabetic nephropathy—namely, albumin, creatinine, and glucose—within a single analytical run. Unlike conventional flow-based techniques that rely on sequential measurements, the proposed CIA platform integrates multiple analytical pathways into a unified design, enabling one-shot multi-analyte analysis without the need for complex separation units or injection valves. The system employs peristaltic pumps and a rectangular platform with orthogonal flow channels, allowing concurrent aspiration and efficient transport of reaction products to compact detectors. Albumin determination was based on ion-association with tetrabromophenolphthalein ethyl ester. Creatinine was measured using the Jaffé reaction. Glucose was colorimetrically detected via its reaction with 3,5-dinitrosalicylic acid. The developed CIA provides enhanced sensitivity through its pre-mixing effect, enabling reliable quantification of trace analytes. Excellent analytical performance was achieved, including wide linear ranges (r<sup>2</sup> > 0.99), good precision (RSD < 7%), and rapid analysis (5 min). The method was validated against established reference methods, showing no significant differences, and successfully applied to urine with satisfactory recoveries (84.8–107.3%). Importantly, the proposed system adheres to green chemistry by minimizing reagent consumption and waste generation, offering a sustainable approach for multi-parameter clinical analysis.
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    A Microfluidic Paper-based Analytical Device for Simultaneous Measurement of Albumin, Creatinine and Uric Acid in Urine based on Standard Addition Method
    (2024-01-03)
    Wisaichon, Wannakan
    ;
    Choengchan, Nathawut
    ;
    Praditweangkum, Wiboon
    This work describes a simple method for the simultaneous measurement of urinary albumin, creatinine and uric acid using a standard addition approach on a microfluidic paper-based analytical device (µPAD). Hydrophobic barrier of the µPAD was created by stamping indelible ink onto a filter paper. The µPAD was designed with a flower-liked configuration. After aliquoting urine to a central sample zone, the sample flowed to ten surrounding channels, namely “the inner channels”, where the blank (water) or standard solutions had been added. The inner channels were connected to circular reagent zones where reagents had been immobilized. Tetrabromophenolphthalein ethyl ester, alkaline picrate, and a mixed solution of ferric chloride and ferric cyanide were used as the chromogenic reagents for the colorimetric detections of albumin, creatinine, and uric acid, respectively. Each reagent zone was linked with a circular detection zone through a second channel, namely “the outer channels”. The blue-, orange-and greenish-blue colored products were observed in the detection zones for the measurement of albumin, creatinine, and uric acid, correspondingly. A digital image of the µPAD was captured with a mobile phone. The color intensities were evaluated by ImageJ™ and were employed for the quantitative analyses. The standard addition calibrations were found to be linear (r<sup>2</sup> > 0.99) for the spiked analyte concentrations up to 100 mg L<sup>-1</sup> albumin, 1000 mg L<sup>-1</sup> creatinine, and 50 mg dL<sup>-1</sup> uric acid. The paper platform provided high precision (RSD < 5 %) and good analytical recovery (91.8-109.7 %). Under paired t-test, the results obtained by the developed µPAD and the validating methods were not significantly different at 95 % confidence (albumin: t<inf>stat</inf> =-0.130, t<inf>cri</inf> = 3.182, creatinine: t<inf>stat</inf> =-0.133, t<inf>cri</inf> = 3.182, and uric acid: t<inf>stat</inf> = 1.119, t<inf>cri</inf> = 3.182).