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Author: search.filters.author.Deewatthanawong, R.Subject: search.filters.subject.tissue culture

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    Effect of BA and IBA on micropropagation of Cannabis sativa L. ‘Hang Kra Rog’
    (2024-09-01)
    Singhavorachai, P.
    ;
    Deewatthanawong, R.
    ;
    Montri, N.
    ‘Hang Kra Rog’ is an original Thai strain of cannabis (Cannabis sativa L.). Tissue culture methods can rapidly propagate disease-free plants. This study optimized the protocol for surface sterilization and in vitro plant regeneration from explant nodal segments for the propagation of cannabis on an industrial scale. Various Clorox concentrations and soaking periods were used for surface sterilization, with different concentrations of BA and IBA plant growth regulators added to the basal medium for micropropagation of C. sativa L. using nodal explant culture. Results showed that 10% Clorox for 20 min followed by 5% Clorox for 20 min gave the highest explant survival at 75%. Optimal shoot and root development occurred when enriched with 0.5 mg L<sup>-1</sup> IBA, resulting in 1.00 shoots, 3.10 roots, 9.97 leaves, and a shoot length of 4.74 cm. The 2 mg L<sup>-1</sup> BA yielded the highest number of shoots at 1.87 shoots and resulted in a large callus size of 1.19 cm and callus fresh weight of 0.32 g.
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    Effect of plant growth regulators on in vitro seedling growth of Papilionanthe hookeriana (Rchb.f.) Schltr.
    (2023-02-01)
    Montri, N.
    ;
    Charoensrimuang, S.
    ;
    Deewatthanawong, R.
    ;
    Montri, W.
    ;
    Bunya-Atichart, K.
    The effect of plant growth regulators on the in vitro seedling growth of Papilionanthe hookeriana (Rchb.f.) Schltr. was evaluated. The protocorms were cultured in VW media supplemented with combinations of indole acetic acid (IAA) and thidiazuron (TDZ) at the concentrations of 0, 0.1 and 0.2 mg L<sup>-1</sup>. The 2 leaf stage seedlings were cultured on VW media supplemented with cytokinins; 6-furfurylaminopurine (kinetin: K), benzyladenine (BA) and TDZ or auxins; IAA, indole-3-butyric-acid (IBA), α-naphthalene acetic acid (NAA) at the concentrations of 0, 2 and 4 mg L<sup>-1</sup> for 2 months. The results found that combinations of IAA and TDZ had higher protocorm fresh weight when compared with control. Adding with 0.1 and 0.2 mg L<sup>-1</sup> IAA alone were promoted protocorm fresh weight. On the other hand, combinations of 0.1 mg L<sup>-1</sup> IAA and 0.2 mg L<sup>-1</sup> TDZ also improved the fresh weight number. The combinations of TDZ and IAA treatments were achieved for the number of 1<sup>st</sup> stage protocorm. For 2 leaf stage seedling culture, treated with cytokinins was not significantly different in shoot length, seedling fresh weight and leaf number. However, cytokinins suppressed root number compared to untreated. The maximum root numbers at 4.21 roots were found in free cytokinins medium. Seedling fresh weight and leaf numbers were significantly increased when increased the concentration of auxins. Our results indicated that auxins and cytokinins played an instrumental role for P. hookeriana seedling growth.