Montri, Nattaya

Preferred name

Montri, Nattaya

Alternative Name

Montri, N.

Main Affiliation

School of Agricultural Technology

nattaya.mo@kmitl.ac.th

Now showing 1 - 10 of 27

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Antagonistic activity of Candida utilis SCKU1 yeast against crown rot disease of 'Hom Thong' Banana (Musa acuminata, AAA group)
(2022-07-01)
Tongsri, V.
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Sanosomneng, K.
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Umrung, S.
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Montri, N.
Candida utilis SCKU1 yeast had an antifungal activity on seven isolates of fruit postharvest pathogens ranging from 21.5-49.1% inhibition. It was the most effective antagonist against two pathogens, Colletotrichum musae BCm1 causing banana anthracnose and Lasiodiplodia pseudotheobromae DL1 causing durian fruit rot. Inhibition zone appearances on co-culture plates were observed on four pathogens including C. gloeosporioides DCg1, C. musae BCm1, L. pseudotheobromae DL1 and Phomopsis sp. isolate DP1. The largest zone of inhibition (10.15±0.75 mm wide) was noticed in L. pseudotheobromae DL1 plate. Yeast culture filtrate was able to inhibit the mycelial growth of all pathogens, but the most inhibition was found in four pathogens, C. gloeosporioides DCg1, C. gloeosporioides MCg1, C. musae BCm1 and Phomopsis sp. isolate DP1 with 80.6-84.2% inhibition. Additionally, both yeast cell suspension and its culture filtrate significantly reduced the disease severity of banana crown rot by 35.9 and 33.5%, respectively. However, the mechanism of antagonistic yeast against crow rot disease was demonstrated by producing cell wall degrading enzymes such as beta-1,3-glucanase and chitinase. Cell suspension and culture filtrate of yeast also provided the induction of disease resistance by stimulating the activity of the defense-related enzyme, phenylalanine ammonia-lyase (PAL), beta-1,3-glucanase and chitinase against the disease. PAL activity was greatly activated by both treatments at 72 h after application (haa). The activity of beta-1,3-glucanase and chitinase was also induced by both treatments starting from 48 haa, but the highest level of both enzyme activities was demonstrated at 96 haa. At 96 haa, the level of chitinase activity was extremely enhanced by culture filtrate of yeast. This preliminary study revealed that either yeast cell suspension or its culture filtrate might be alternatively used for controlling banana crow rot on banana production.
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Callus induction of stemona curtisii Hook. f.
(2012-10-31)
Montri, N.
;
Chaisriha, S.
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Suwanphakdee, C.
A successful protocol was established for callus induction and regeneration of Stemona curtisii Hook. f. The sterilized seeds were cultured on Murashige and Skoog (MS, 1962) supplemented with 0-0.5 mg L-1 2,4-dichlorophenoxyacetic acid (2,4-D) in dark and light conditions. The combination of 0-2 mg L-1 of 2,4-D and benzyladenine (BA) or thidiazuron (TDZ) was also examined in seed and shoot culture. The results show that callus induction was highest from seed. The 0.4 mg L-1 2,4-D in the dark condition treatment was suitable to promoted callus formation at 62.50%. The combination of 2,4-D with BA or TDZ strongly induced callus formation (2 mg L-1 BA with 2 mg L-1 2,4-D). Plant regeneration from callus was successfully induced via both organogenesis and embryogenesis.
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Callus and hairy root induction of Melaleuca cajuputi Powell
(2020-05-01)
Prangthong, U.
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Montri, N.
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Saetiew, K.
It is stated that the lowest percentage of contamination at 49.63% and highest percentage of survival at 37.95% after sterilized with 70% ethanol for 1 min and 1.8 % NaOCl for 30 min. The bud explants were cultured on Murashige and Skoog (MS) medium supplemented with 0, 0.02, 0.04, 0.06, 0.08 mg/l 2,4-Dinitrophenylhylhydrazine (2,4-D) combination with 0, 0.02, 0.04, 0.06, and 0.08 mg/l Thidiazuron (TDZ) for callus induction. The results showed that the explants were cultured on MS medium supplemented with 0.06 mg/l TDZ resulting to be suitable for callus induction with biggest size at 65.2x1. 22cm of width and length. Culturing buds on MS medium supplemented with 0.02 mg/l TDZ had the highest averaged shoot number. The roots were cultured in liquid MS medium supplemented with 0.04 mg/l TDZ, 0.04 mg/l 2.4-D gave the highest weight of hairy roots.
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Exploring Tectona grandis Linn. f. Leaf Extract as a Functional Feed Additive with Antioxidant and Nutraceutical Potential for Livestock
(2025-12-01)
Montri, Nattaya
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Wanapat, Metha
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Kang, Sungchhang
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Cheas, Seangla
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Cherdthong, Anusorn
This study investigated the phytochemical composition, antioxidant activity, and safety of Tectona grandis leaf extracts at four maturity stages. Ethanolic extracts were screened for secondary metabolites and analyzed using GC–MS, together with heavy metal determination, phenolic profiling, and antioxidant assays. Preliminary phytochemical screening revealed the presence of alkaloids, flavonoids, glycosides, tannins, saponins, and coumarins, with clear variation among leaf stages. GC–MS analysis detected multiple bioactive constituents, including phytol, n-decanoic acid, and heptadecenal. These compounds have been previously reported to exhibit antioxidant, antimicrobial, and anti-inflammatory properties. Heavy metal analysis showed very low cadmium concentrations (0.001–0.004 mg/kg), undetectable lead levels (<LOD), and trace levels of arsenic concentrations (0.012–0.018 mg/kg), all of which were within safe limits for plant materials. Quantitative assays demonstrated that mature leaves contained the highest total phenolic (8.751 ± 0.018 mg GAE/g DW), total flavonoid (0.359 ± 0.017 mg QE/g DW), and condensed tannin (0.303 ± 0.000 mg CE/g DW) contents. Correspondingly, mature-leaf extracts exhibited the greatest antioxidant capacity, showing 95.88 ± 0.12% inhibition of oxidant activity. The ethanolic extracts exhibited IC<inf>50</inf> values ranging from 22.93–50.35 mg/mL, whereas the ascorbic acid standard showed an IC<inf>50</inf> of 0.0767 mg/mL, confirming the expected lower potency of crude plant extracts. The strong correlation between phenolic accumulation and antioxidant activity suggests enhanced defense against oxidative stress. The absence of detectable heavy metal contamination, together with the high polyphenolic content, suggests that T. grandis leaves may serve as a safe natural source of antioxidants. These findings demonstrate the strong antioxidant potential of T. grandis leaf extracts, providing a basis for future studies evaluating their functional applications in biological systems.
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Effects of organic compound and plant growth regulators on seed germination and growth of Doritis pulcherrima Lindl. in vitro
(2024-09-01)
Supreeworakij, S.
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Saetiew, K.
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Rungkitwattananukul, T.
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Phanchompoo, P.
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Montri, N.
Doritis pulcherrima Lindl. is one of the wild orchids of which the distribution has decreased in nature and endangered. In vitro seed culture by using plant tissue culture is a proper method to help increasing the numbers. The purpose of this research was to study the effect of organic compound and plant growth regulators on seed germination and growth of D. pulcherrima in vitro. Seeds were cultured on Vacin and Went (VW) basal medium. Experiments were performed in a 5×7 factorial in completely randomized design (factorial in CRD) 2 factors; Factor 1 capsule of D. pulcherrima with 5 treatments such as Pi102/1, Pi102/2, Pi119/1, Pi119/2 and Pu112. Factor 2 organic compound with 7 treatments such as no organic compound (control), 50, 100 g L-1 raw namwa banana powder, 50, 100 g L-1 cavendish banana homogenate, 50, 100 g L-1 potato homogenate. After 13 weeks of culture, there were only 3 capsules growth seed germination (Pi119/2, Pi102/2 and Pu112/1) and the highest percentage of seed germination was Pi119/2 on VW medium supplemented with 50, 100 g L-1 banana powder and 50 g L-1 potato homogenate gave the highest germination percentage at level 4 (75-100%). From the results, organic compound is a suitable culture supplement for D. pulcherrima seed germination and development compared to no organic compound. In addition, the effect of plant growth regulators on growth and development of D. pulcherrima protocorm were cultured on VW medium supplemented with different concentrations of control, 1, 2 and 3 mg L-1 BA and 0.01, 0.02 and 0.03 mg L-1TDZ. The result showed the control gave the highest number of leaves at 6.50, the number of protocorm at 3.67 and fresh weight of protocorm at 0.42 g. The protocorm and plantlet were induced after 10 of culture.
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In vitro propagation of Stemona tuberosa Lour., an antitussive medicinal herb
(2009-02-28)
Montri, N.
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Wawrosch, Ch
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Kopp, B.
In vitro propagation protocol of Stemona tuberosa Lour., an antitussive medicinal herb was developed using shoot culture. Shoots were induced from in vitro shoot and node explants in solid MS (Murashige and Skoog, 1962) medium supplemented with 20 μM BAP. Two types of media, B5 and MS, and different concentrations of MS media were tested for the screening of suitable media. Amongst them, full strength of MS was the most effective for in vitro culture. For the step of shoot multiplication, shoots were cultured in MS media supplemented with various types and concentrations of cytokinins. Sixteen combinations of BAP (0-20 μM) and IBA (0-5 μM), were also tested. Shoot numbers from explants were examined in all media. The highest number of shoot multiplication rate could be observed on solid MS medium containing 20 μM BAP. Shoots were cultured on MS media supplemented with different types and concentration of auxins for root induction. Regenerated shoots were successfully rooted after cultured on solid MS medium which were without plant growth regulators for 2 months. Plantlets could be established and grown well in the greenhouse.
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Influence of geographical and genetic variation on GABA content in Annona muricata grown in Thailand
(2020-12-08)
Deewatthanawong, R.
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Tontiworachai, B.
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Kongchinda, P.
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Chanapan, S.
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Sriwilaiwan, S.
Annona is a genus of tropical fruit trees in the family Annonaceae. Fruit pulp of Annona muricata contained the highest amount of gamma-aminobutyric acid (GABA) among Annona species. GABA content results from many factors including genetic makeup and environment. Influences of genetic variation and geographical environment on GABA content in fruit pulp of A. muricata were determined. To study genetic variation, sequences of ITS2 and psbA-trnH were used to construct phylogenetic trees. Young leaves of A. muricata were collected from different geographical locations including Chumphon, Trang, Nakhon Ratchasima, Nakhon Si Thammarat, Buriram, Nong Khai, Udon Thani, and Prachuap Khiri Khan Provinces in Thailand. Sequences of ITS2 and psbA-tmH were aligned and data sets were analyzed using maximum likelihood (ML) and neighbor-joining (NJ) methods. ITS2 sequence variations were observed within and among samples from different locations. A. muricata fruit harvested from eight provinces, namely Chumpon, Trang, Nakhon Ratchasima, Nakhon Si Thammarat, Buriram, Nong Khai, Udon Thani, and Prachuap Khiri Khan contained 19.75, 35.02, 32.91, 28.18, 42.37, 32.17, 65.02, and 31.58 mg 100 g-1 FW GABA, respectively. Findings indicated that both geographical environment and genetic variation had significant effects on GABA content in A. muricata fruit.
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Influence of exogenous salicylic acid on phytochemical improvement and antioxidant activity in Cannabis sativa L.
(2024-01-01)
Tebdoie, C.
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Dewatthanawong, R.
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Kongjinda, P.
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Montri, N.
The impact of salicylic acid (SA) foliar spray at varied concentrations (0 and 1 M) and preharvest periods (24, 36, and 42 hours) on secondary metabolite in inflorescences of cannabis (Cannabis sativa L.) was investigated. Result was significantly differed in secondary compound composition. Application of 1 M SA at 24 hours preharvest increased total phenolics, total flavonoids, and total chlorophyll. Moreover, 1 M SA at 24 and 42 hours preharvest showed higher chlorophyll a and b contents than 1 M SA at 36 hours preharvest. While antioxidant activity was not significantly differed among 1 M SA treatments at different preharvest periods, it surpassed non-SA-treated plants. The total pigment was not significantly differed among SA treatments. 1 M SA spray reduced carotenoid content in cannabis inflorescences, with the highest carotenoid observed in non-SA treated plants.
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Correlation between structure, chromaticity, and dielectric properties of calcium copper pyrophosphates, Ca2−xCu xP2O7
(2022-12-01)
Baitahe, Rattanai
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Sronsri, Chuchai
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Thompho, Somphob
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Chaiseeda, Kittichai
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Montri, Nattaya
The solid-state reaction was employed to synthesize Ca<inf>2−x</inf>Cu<inf>x</inf>P<inf>2</inf>O<inf>7</inf> by varying the mole ratio between Ca and Cu. The structure and crystallography of the pyrophosphate compounds were identified and confirmed by using X-ray diffraction (XRD). The Rietveld refinement method and the extended X-ray absorption fine structure (EXAFS) least-squares fitting technique were also applied to refine the sample crystal structure. The single phases of the obtained Ca<inf>2</inf>P<inf>2</inf>O<inf>7</inf>, CaCuP<inf>2</inf>O<inf>7</inf>, and Cu<inf>2</inf>P<inf>2</inf>O<inf>7</inf> samples and the mixing phases of the obtained Ca<inf>1.5</inf>Cu<inf>0.5</inf>P<inf>2</inf>O<inf>7</inf> and Ca<inf>0.5</inf>Cu<inf>1.5</inf>P<inf>2</inf>O<inf>7</inf> samples were identified, and then only a single phase of the samples was subjected to structural and dielectrical analyses. The structural results exhibit the tetragonal crystal system with the P4<inf>1</inf> space group for β-Ca<inf>2</inf>P<inf>2</inf>O<inf>7</inf>, the monoclinic crystal system with the P2<inf>1</inf>/c space group for CaCuP<inf>2</inf>O<inf>7</inf>, and the C2/c space group for α-Cu<inf>2</inf>P<inf>2</inf>O<inf>7</inf>. The dielectric constant (ε<inf>r</inf>) of the single metal pyrophosphates (Ca<inf>2</inf>P<inf>2</inf>O<inf>7</inf> and Cu<inf>2</inf>P<inf>2</inf>O<inf>7</inf>) was higher than that of binary metal pyrophosphates (CaCuP<inf>2</inf>O<inf>7</inf>). The image sensor result of the Cu<inf>2</inf>P<inf>2</inf>O<inf>7</inf> sample (x = 2.00) illustrated a yellowish-green color, while other compounds (x = 0.50−1.50) presented color tones that changed from blue-green to bluish-green. Raman and Fourier transform infrared (FTIR) spectrophotometers were employed to characterize and confirm the vibrational characteristics of the P<inf>2</inf>O<inf>7</inf>4− group, which contains the O–P–O radical ([PO<inf>2</inf>]-) and the P–O–P bride ([OPO]-) and approximate M–O stretching modes. Furthermore, this work reports for the first time that the change in the crystal structure of Ca<inf>2−x</inf>Cu<inf>x</inf>P<inf>2</inf>O<inf>7</inf> (i.e., bond angle of P−O−P in P<inf>2</inf>O<inf>7</inf>4− and distortion phenomena in the M−O<inf>6</inf> octahedral site) are cause the correlation between the structure, chromaticity, and dielectric properties of calcium copper pyrophosphates, Ca<inf>2−x</inf>Cu<inf>x</inf>P<inf>2</inf>O<inf>7</inf>.
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Ensiling Time and Mixed Microbe Fermented Liquid Modulate In Vitro Digestibility and Rumen Fermentation of Fermented Total Mixed Rations
(2026-01-01)
Polyorach, Sineenart
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Suphalucksana, Wichai
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Klompanya, Ampon
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Yuangklang, Chalermpon
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Wanapat, Metha
This study investigated how varying the ensiling period and the level of mixed microbes fermented liquid (MMFL) influences the chemical composition, in vitro degradability, rumen fermentation profile, and microbial ecology of fermented total mixed rations (FTMR). A completely randomized 4 × 4 factorial design was used, incorporating four fermentation durations (0, 7, 14, and 21 days) and four MMFL inclusion rates (0, 0.5, 1.0, and 1.5% of DM). Both factors exerted significant effects on FTMR quality (p < 0.05). The combination of a 14-day fermentation period with 0.5% MMFL consistently produced the most desirable outcomes. Under these conditions, crude protein concentration rose from 12.0% to 14.3% of DM, while neutral-detergent fiber declined from 54.2% to 49.1%. Improvements were also observed in in vitro digestibility, with DM and OM increasing by 9–12% relative to the untreated control (p < 0.05). Fermentation end-products were enhanced, as total volatile fatty acids increased by 15% (92.4 vs. 80.1 mmol/L), and the molar proportion of propionate increased from 24.5 to 29.2 mol/100 mol, thereby lowering the acetate-to-propionate ratio (2.4 vs. 3.0; p < 0.05). Estimated methane production declined by 18% (p < 0.01). Microbial counts reflected a shift toward a more efficient fermentative community, with bacterial and fungal populations increasing by 21% and 18%, and protozoa decreasing by 25% (p < 0.05). Overall, moderate MMFL supplementation during a 14-day ensiling phase enhanced nutrient conservation and fermentation efficiency, suggesting practical value for improving FTMR utilization in ruminant systems. Further in vivo and economic evaluations remain necessary.
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Montri, Nattaya

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