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Author: search.filters.author.Intaraudom, ChakapongSubject: search.filters.subject.Actinomycetes

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    Streptomyces telluris sp. nov., a promising terrestrial actinobacterium with antioxidative potentials
    (2023-06-01)
    Thayanuwadtanawong, Onnicha
    ;
    Duangupama, Thitikorn
    ;
    Bunbamrung, Nantiya
    ;
    Pittayakhajonwut, Pattama
    ;
    Intaraudom, Chakapong
    An actinomycete strain, AA8<sup>T</sup>, which produced a long straight chain of spores (verticillati type), was isolated from the rhizosphere soil of Mangifera indica in Bangkok, Thailand. A polyphasic taxonomic study was carried out to establish the taxonomic position of the strain. Strain AA8<sup>T</sup> formed a tight taxonomic position in the 16S rRNA gene tree with Streptomyces roseifaciens MBT76<sup>T</sup>. In contrast, the genome-based taxonomic analysis showed that strain AA8<sup>T</sup> shared low average nucleotide identity-BLAST (94.1%), the digital DNA–DNA hybridization (58.2%), and the average amino acid identity (93.6%) values with S. roseifaciens MBT76<sup>T</sup>. Moreover, a combination of physiological and biochemical properties indicated that strain AA8<sup>T</sup> was distinguished from all Streptomyces species with effectively published names. Strain AA8<sup>T</sup>, therefore, represents a novel species of Streptomyces, and the name Streptomyces telluris is proposed for the strain. The type strain is AA8<sup>T</sup> (= TBRC 8483<sup>T</sup> = NBRC 113461<sup>T</sup>). The chemical investigation led to the isolation of nine known compounds (compounds 1–9). Among these compounds, compound 7 (3,4-dihydroxybenzaldehyde) possesses strong antioxidant activity equal to ascorbic acid, a powerful antioxidative agent.
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    Abyssomicin derivatives from the rhizosphere soil actinomycete Microbispora rhizosphaerae sp. nov. TBRC6028
    (2021-05-01)
    Bunbamrung, Nantiya
    ;
    Kittisrisopit, Suchada
    ;
    Intaraudom, Chakapong
    ;
    Dramae, Aibrohim
    ;
    Thawai, Chitti
    Three undescribed abyssomicin derivatives, including microbimisin, abyssomicins Z1, and Z2, were isolated from the soil actinomycete Microbispora rhizosphaerae sp. nov. TBRC6028. Chemical structures were determined by NMR spectroscopic data (<sup>1</sup>H, <sup>13</sup>C, COSY, HSQC, HMBC, and NOESY spectra) and the absolute configurations were verified by single-crystal X-ray diffraction analyses together with the ECD spectral data. Microbimisin and abyssomicin Z1 exhibited weak antibacterial activity against Bacillus cereus with MIC values of 25.0 and 50.0 μg/mL without cytotoxicity against MCF-7 and Vero cells at the concentration of 50 μg/mL.
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    Streptomyces musisoli sp. nov., an actinomycete isolated from soil
    (2021-01-01)
    Duangupama, Thitikorn
    ;
    Intaraudom, Chakapong
    ;
    Pittayakhajonwut, Pattama
    ;
    Suriyachadkun, Chanwit
    ;
    Tadtong, Sarin
    An actinobacterium, strain CH5-8<sup>T</sup>, which formed spiral chains of spore arising from the aerial mycelium, was isolated from rhizosphere soil of Musa spp. The organism exhibited vivid greenish yellow substrate mycelium and easily produced the medium grey aerial spore mass on ISP2 medium. The typical chemotaxonomic properties of members of the genus Streptomyces were observed for strain CH5-8<sup>T</sup>, e.g. ll-diaminopimelic acid in cell peptidoglycan, MK-9(H<inf>8</inf>), MK-9(H<inf>6</inf>), and MK-9(H<inf>4</inf>) as major menaquinones and anteiso-C<inf>15:0</inf>, iso-C<inf>16:0</inf>, and anteiso-C<inf>17:0</inf> as major fatty acids. Diphosphatidylglycerol, phosphatidylethanola-mine, hydroxyphosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannoside were detected in the cells. A combination of morphological and chemotaxonomic data supported the assignment to the genus Strep-tomyces. The analysis result obtained for the 16S rRNA gene sequence confirmed the taxonomic affiliation at the genus level of this strain. The novel strain CH5-8<sup>T</sup> showed the highest 16S rRNA gene sequence values to Streptomyces echinatus NBRC 12763<sup>T</sup> (98.9%), followed by Streptomyces actinomycinicus RCU-197<sup>T</sup> (98.9%). The average nucleotide identity by blast (ANIb) and digital DNA–DNA hybridization values between CH5-8<sup>T</sup> and its closest relatives, S. echinatus CECT 3313<sup>T</sup> and S. actinomycinicus RCU-197<sup>T</sup>, were ≤91.6% and ≤47.4%, respectively. The digital DNA G+C content of genomic DNA was 72.1 mol%. On the basis of these phenotypic and genotypic data, strain CH5-8<sup>T</sup> represents a novel species, for which the name Streptomyces musisoli sp. nov. is proposed. The type strain is CH5-8<sup>T</sup> (=TBRC 9950<sup>T</sup>=NBRC 113997<sup>T</sup>).
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    Antibacterial, antitubercular, antimalarial and cytotoxic substances from the endophytic Streptomyces sp. TBRC7642
    (2020-04-01)
    Bunbamrung, Nantiya
    ;
    Intaraudom, Chakapong
    ;
    Dramae, Aibrohim
    ;
    Thawai, Chitti
    ;
    Tadtong, Sarin
    Eight previously undescribed naturally-occurring compounds, including abyssomycins Y - Z, methyl aeruginoate, desferri-ferrithocin-4-hydroxyphenethylester, streptomethiocins A - B, furaquinocin I, and streptolactone, along with eleven known compounds were isolated from the endophytic Streptomyces sp. TBRC7642. The chemical structures were determined based on spectroscopic means including 1D, 2D NMR spectroscopy and mass spectrometry. The absolute configurations were assigned by relying on CD spectra and their optical rotations. In addition, the isolated compounds were evaluated for biological activity, such as antimalarial, antitubercular, antibacterial (both Gram-positive and Gram-negative bacteria), as well as for cytotoxicity against MCF-7, NCI–H187, and Vero cells.