KMITL

Permanent URI for this communityhttps://dspace.kmitl.ac.th/handle/123456789/1

Browse

Settings

Author: search.filters.author.Kaewsaneha, ChariyaSubject: search.filters.subject.Antibacterial

Search Results

Now showing 1 - 2 of 2
  • Loading...
    Thumbnail Image
    Some of the metrics are blocked by your 
    consent settings
    Item type:Publication,
    PMMA particles coated with chitosan-silver nanoparticles as a dual antibacterial modifier for natural rubber latex films
    (2019-02-01)
    Suteewong, Teeraporn
    ;
    Wongpreecha, Jitrada
    ;
    Polpanich, Duangporn
    ;
    Jangpatarapongsa, Kulachart
    ;
    Kaewsaneha, Chariya
    The antibacterial activity in sulphur prevulcanized natural rubber (SPNR) latex film was effectively improved by deposition of poly(methyl methacrylate) (PMMA) particles encircled with chitosan-coated silver nanoparticles (AgNPs-CS). With the focus on a green process, CS was selected as a safe reducing and stabilizing agent for the one-step synthesis of AgNPs-CS (38 nm, +40.4 mV) in an autoclave. The adsorption of small-sized AgNPs-CS directly onto rubber film did not provide an inhibitory effect on S. aureus. It also had a low antibacterial effect on E. coli. This is because of the particles becoming completely/partially submerged into the soft rubber matrix upon drying. Hence, the AgNPs-CS were fabricated as a shell surrounding a rigid PMMA core (496 nm, -30.9 mV). This was done using a heterocoagulation technique prior to coating on SPNR film. The presence of PMMA/AgNPs-CS on the surface of SPNR film effectively increased the surface roughness from ca. 44 to 150 nm. This substantially promoted the antibacterial activity against E. coli and S. aureus by way of contact killing and repelling mechanisms. The cytotoxicity on L-929 fibroblasts was also suppressed. This study would be, therefore, applicable to the development of antibacterial SPNR film with high surface roughness, low cytotoxicity. It could also be applied for other soft substrates.
  • Loading...
    Thumbnail Image
    Some of the metrics are blocked by your 
    consent settings
    Item type:Publication,
    One-pot, large-scale green synthesis of silver nanoparticles-chitosan with enhanced antibacterial activity and low cytotoxicity
    (2018-11-01)
    Wongpreecha, Jitrada
    ;
    Polpanich, Duangporn
    ;
    Suteewong, Teeraporn
    ;
    Kaewsaneha, Chariya
    ;
    Tangboriboonrat, Pramuan
    Silver nanoparticles stabilized with chitosan (AgNPs-CS) were synthesized based on the one-pot green process in an autoclave, in which CS acts as reducing agent as well as stabilizer. Effects related to temperature and pressure input on particle formation were systematically investigated. Mechanism taking place during particle nucleation and growth was proposed. The data from UV–vis absorption, X-ray diffraction pattern and morphology confirmed the formation of AgNPs-CS with face-centered cubic (fcc) structure. The synthesized AgNPs-CS showed the effective antibacterial activity against both E. coli and S. aureus. The minimum bactericidal concentration values of 39.1 and 312.5 μg/ml for E. coli and S. aureus, respectively, did not show cytotoxicity to L-929 fibroblast. Moreover, the covering of CS on the surface of AgNPs-CS was proven to reduce the cytotoxicity when compared with commercial citrate-stabilized AgNPs. Considering simple and mild process, this synthesis approach would be helpful for development of benign AgNPs-based antibacterial agent.