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Author: search.filters.author.Kittisrisopit, SuchadaSubject: search.filters.subject.Actinomycetes

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    Item type:Publication,
    Abyssomicin derivatives from the rhizosphere soil actinomycete Microbispora rhizosphaerae sp. nov. TBRC6028
    (2021-05-01)
    Bunbamrung, Nantiya
    ;
    Kittisrisopit, Suchada
    ;
    Intaraudom, Chakapong
    ;
    Dramae, Aibrohim
    ;
    Thawai, Chitti
    Three undescribed abyssomicin derivatives, including microbimisin, abyssomicins Z1, and Z2, were isolated from the soil actinomycete Microbispora rhizosphaerae sp. nov. TBRC6028. Chemical structures were determined by NMR spectroscopic data (<sup>1</sup>H, <sup>13</sup>C, COSY, HSQC, HMBC, and NOESY spectra) and the absolute configurations were verified by single-crystal X-ray diffraction analyses together with the ECD spectral data. Microbimisin and abyssomicin Z1 exhibited weak antibacterial activity against Bacillus cereus with MIC values of 25.0 and 50.0 μg/mL without cytotoxicity against MCF-7 and Vero cells at the concentration of 50 μg/mL.
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    Item type:Publication,
    Microbispora soli sp. nov., isolated from soil of a hot spring
    (2018-12-01)
    Kittisrisopit, Suchada
    ;
    Pittayakhajonwut, Pattama
    ;
    Tadtong, Sarin
    ;
    Thawai, Chitti
    A novel actinomycete, strain RTBAU4-9 <sup>T</sup> , which produced longitudinally paired spores on aerial mycelia, was isolated from a soil sample of a hot spring area. This strain exhibited chemotaxonomic and genotypic properties typical of the genus Microbispora, such as meso-diaminopimelic acid in the cell-wall peptidoglycan, glucose, ribose and trace amount of madurose as the characteristic whole-cell sugars, phosphatidylethanolamine, diphosphatidylglycerol and phosphoglycolipids as the membrane phospholipids, MK-9(H <inf>4</inf> ), MK-9(H <inf>2</inf> ) and MK-9(H <inf>0</inf> ) as the characteristic menaquinones, and iso-C <inf>16: 0</inf> and anteiso-C <inf>17: 0</inf> as the main fatty acids. The G+C content of the genomic DNA was 70.2 mol%. The result of 16S rRNA gene sequence analysis revealed the strain was a member of the genus Microbispora and was most closely related to Microbispora hainanensis 211020 <sup>T</sup> (98.3% 16S rRNA gene sequence similarity). In addition, the low percentage of DNA-DNA relatedness (<28.1±1.2 %) and several phenotypic differences confirmed that strain RTBAU4-9 <sup>T</sup> should be considered as representing a novel species of the genus Microbispora, for which the name Microbispora soli sp. nov. is proposed. The type strain is RTBAU4-9 <sup>T</sup> (=TBRC 7648 <sup>T</sup> =NBRC 113147 <sup>T</sup> ).