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Author: search.filters.author.Klamchuen, AnnopSubject: search.filters.subject.Electrochemical sensor

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    Shape memory laser-induced graphene electrode based on polybenzoxazine-co-epoxy coated fabric and its electrochemical sensor application
    (2025-12-01)
    Luengrojanakul, Panuwat
    ;
    Klamchuen, Annop
    ;
    Leepheng, Piyawan
    ;
    Tunhoo, Benchapol
    ;
    Charoensuk, Krittapas
    In this investigation, CO<inf>2</inf> direct laser writing is performed on BA-a/NGDE shape memory polymer matrix coated fabric. Findings show that the quality and surface coverage of formed laser-induced graphene (LIG) are dependent on BA-a content. The LIGs acquired at higher BA-a content possess low sheet resistance (∼10–15 Ω/sq), facilitating its use as joule heater for shape memory actuation (T<inf>avg</inf> ∼130 °C at 20 V for BOZ70-LIG). Furthermore, the fabricated LIG electrode shows potential use as electrochemical sensor with ΔE<inf>p</inf> of 88–200 mV and k<sup>0</sup> of 0.005 cm s<sup>-1</sup> (20 mm length) for 1 mM K<inf>4</inf>[Fe(CN)<inf>6</inf>]/0.1 M KCl. The 40 mm electrode length also maintains respectable electrochemical performance even after subjecting LIG to shape deformation and recovery. Moreover, the molecular imprinted polymer (MIP) modified LIG electrode also exhibits promising results in selective dopamine detection through leucodopaminechrome in PBS with linear detection range of 1.0–15.0 μM and LOD of 0.61 μM.
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    Laser-induced graphene electrochemical immunosensors for rapid and sensitive serological detection: A case study on dengue detection platform
    (2025-06-01)
    Inlumphan, Supawee
    ;
    Wongwiriyapan, Winadda
    ;
    Khemasiri, Narathon
    ;
    Rattanawarinchai, Prapakorn
    ;
    Leepheng, Piyawan
    Here, we present a diagnostic on a chip platform based on laser-induced graphene (LIG) electrochemical immunosensors for serological detection. The electrochemical immunosensors are fabricated through CO<inf>2</inf> laser induction on polyimide (PI) sheets. Optimal electrochemical activity of LIG electrodes is obtained under optimized conditions of laser fluence. To verify the application, the serological detection platform was demonstrated. After functionalization with dengue virus (DENV) antigen, the LIG electrochemical immunosensors are able to sense the presence of mouse anti-flavivirus monoclonal (4G2) antibody in a wide linear working range of 25–20,000 ng/ml with the limit of detection (LOD) of 17.41 ng/ml. A specific recognition with 4G2 antibodies against with media protein and isotype is confirmed. Furthermore, the reliability of LIG electrochemical immunosensors compared to conventional enzyme-linked immunosorbent assay (ELISA) is verified through the NS1 antibodies identification in human blood serum clinical samples at room temperature. Our results highlight that the LIG-based electrode is a promising platform for electrochemical immunosensors, aimed at developing reliable and practical diagnostic tools for serological detection. These tools enable early diagnosis of infectious diseases, as well as non-invasive and rapid screening.