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Effect of calcium phosphate on growth, pigment and secondary compound accumulation in Cannabis sativa L. callus
(2026-05-01)
Tebdoie, C.
;
Thipphaaut, T.
;
Deewatthanawong, R.
;
Kongchinda, P.
;
Singhavorachai, P.
The application of calcium phosphate in the form of tricalcium phosphate (Ca3(PO4)2) on cannabis (Cannabis sativa L.) callus cultures for 7 days significantly increased fresh and dry weights at concentrations ranging from 800 to 2000 mg/L, with no statistical differences between concentrations. However, higher calcium phosphate concentrations led to reductions in pigments and DPPH radical scavenging activity. By contrast, callus cultures treated with 800 and 1600 mg/L showed enhanced total phenolic content and carotenoids, while total flavonoid content increased proportionally. These findings suggested that calcium phosphate concentration influenced the biosynthesis of secondary metabolites in cannabis callus cultures.
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Optimizing simplified growing media to enhance cannabis cultivation
(2025-07-01)
Tebdoie, C.
;
Khana, A.
;
Pajareeyanont, S.
;
Deewatthanawong, R.
;
Kongchinda, P.
This study evaluated the effects of different growing media on the vegetative growth, chlorophyll content, and inflorescence yield of Cannabis sativa L. ‘Platinum Kush Breath Remix’. A substrate composed of peat moss, perlite, and vermiculite (3:1:1; PM) significantly enhanced plant height, stem diameter, number of nodes, and inflorescence yield. The highest chlorophyll a and total chlorophyll contents were observed in plants grown in a medium comprising coconut coir, perlite, vermiculite, and vermicompost (3:1:1:1; CC1), which was comparable to PM in terms of yield. Both PM and CC1 media significantly outperformed other treatments, indicating their suitability for optimizing C. sativa growth and productivity.
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Non-destructive measurement of Tetrahydrocannabinol (THC) and Cannabidiol (CBD) using near-infrared spectroscopy
(2023-11-01)
Deewatthanawong, R.
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Kongchinda, P.
;
Chanapan, S.
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Tontiworachai, B.
;
Sakkhamduang, C.
Tetrahydrocannabinol (THC) and cannabidiol (CBD) are cannabinoids which produced by cannabis plants and major compounds found in cannabis products. A predictive method for non-destructive quantification of THC and CBD using near infrared spectroscopy (NIR) technology is developed. The prediction model for THC estimation had coefficient of determination (R-squared) and root mean square error of calibration (RMSEC) values of 0.9994 and 0.1926, respectively. The correlation between THC values of HPLC measurement and NIR prediction showed a correlation coefficient of 0.9078. For CBD prediction, the R-squared and RMSEC values of CBD equation were 0.9995 and 0.0006, respectively. The predicted and measured concentrations of CBD showed good correlation with a regression correlation of 0.9413. The test indicated NIR could be a promising alternative method for THC and CBD evaluation.
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Influence of geographical and genetic variation on GABA content in Annona muricata grown in Thailand
(2020-12-08)
Deewatthanawong, R.
;
Tontiworachai, B.
;
Kongchinda, P.
;
Chanapan, S.
;
Sriwilaiwan, S.
Annona is a genus of tropical fruit trees in the family Annonaceae. Fruit pulp of Annona muricata contained the highest amount of gamma-aminobutyric acid (GABA) among Annona species. GABA content results from many factors including genetic makeup and environment. Influences of genetic variation and geographical environment on GABA content in fruit pulp of A. muricata were determined. To study genetic variation, sequences of ITS2 and psbA-trnH were used to construct phylogenetic trees. Young leaves of A. muricata were collected from different geographical locations including Chumphon, Trang, Nakhon Ratchasima, Nakhon Si Thammarat, Buriram, Nong Khai, Udon Thani, and Prachuap Khiri Khan Provinces in Thailand. Sequences of ITS2 and psbA-tmH were aligned and data sets were analyzed using maximum likelihood (ML) and neighbor-joining (NJ) methods. ITS2 sequence variations were observed within and among samples from different locations. A. muricata fruit harvested from eight provinces, namely Chumpon, Trang, Nakhon Ratchasima, Nakhon Si Thammarat, Buriram, Nong Khai, Udon Thani, and Prachuap Khiri Khan contained 19.75, 35.02, 32.91, 28.18, 42.37, 32.17, 65.02, and 31.58 mg 100 g-1 FW GABA, respectively. Findings indicated that both geographical environment and genetic variation had significant effects on GABA content in A. muricata fruit.
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GC-MS analysis and biopesticide properties of different crude extracts of Annona squamosa and Annona muricata
(2019-01-01)
Deewatthanawong, R.
;
Kongchinda, P.
;
Deewatthanawong, P.
;
Pumnuan, J.
;
Insung, A.
Most common compounds identified by GC-MS analysis of ethanolic extracts from leaves of Annona squamosa (sweetsop) and Annona muricata (soursop) were neophytadiene, hexadecanoic acid, and trans-caryophyllene. Hexadecenoic acid and ethyl oleate were identified in seeds of A. muricata, while only methyl-p-tert-butyl phenylacetate was identified in seeds extract of A. squamosa. Crude extracts were tested for their antifungal activities against postharvest plant pathogens, insecticidal activities against brown planthopper, Nilaparvata lugens (Stål) and cassava mealybug, Phenacoccus manihoti Matile-Ferrero. Results showed low inhibitory effect (less than 20%) for the pathogenic fungi insecticidal test against cassava mealybug of all crude brown planthopper treated with 5% seed extract of A. squamosa, indicating its potential as an alternative botanical insecticide. Further studies are required to improve yield and extract quality.

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