Non-destructive measurement of Tetrahydrocannabinol (THC) and Cannabidiol (CBD) using near-infrared spectroscopy

Abstract

Tetrahydrocannabinol (THC) and cannabidiol (CBD) are cannabinoids which produced by cannabis plants and major compounds found in cannabis products. A predictive method for non-destructive quantification of THC and CBD using near infrared spectroscopy (NIR) technology is developed. The prediction model for THC estimation had coefficient of determination (R-squared) and root mean square error of calibration (RMSEC) values of 0.9994 and 0.1926, respectively. The correlation between THC values of HPLC measurement and NIR prediction showed a correlation coefficient of 0.9078. For CBD prediction, the R-squared and RMSEC values of CBD equation were 0.9995 and 0.0006, respectively. The predicted and measured concentrations of CBD showed good correlation with a regression correlation of 0.9413. The test indicated NIR could be a promising alternative method for THC and CBD evaluation.