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Author: search.filters.author.Limthin, DalawanSubject: search.filters.subject.albumin

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    Item type:Publication,
    Selective electrochemical determination based on magnetic molecularly imprinted polymers for albumin detection
    (2022-06-01)
    Leepheng, Piyawan
    ;
    Limthin, Dalawan
    ;
    Onlaor, Korakot
    ;
    Tunhoo, Benchapol
    ;
    Thiwawong, Thutiyaporn
    Egg allergy is one of the most common allergies in humans. Allergenic egg protein as ovalbumin is the most abundant protein found in egg white. A selective electrode in electrochemical analysis for albumin from egg white was developed based on magnetic molecularly imprinted polymers (albumin-MMIPs) modified on a screen-printed electrode. Surface modification was applied using methyl methacrylate polymer to create surface specificity for albumin protein. The modified surface was characterized by a field-emission scanning electron microscope (FE-SEM). The average pore size was obtained by Brunauer-Emmett-Teller (BET) analysis, and the potentiostat obtained all electrochemical analysis. The albumin-MMIP electrode was selectivity with albumin, comparison with gluten, glycine, casein, and glucose. The modified electrodes present selectivity of 1.13 × 104 μAg-1ml and a limit of detection of 0.18 mg ml-1. The electrode can be applied to albumin protein detection in solution form in food industries.