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    Combined Effect of Acid Salts with Clove and Cinnamon Oils on Controlling of Postharvest Decay in Carrot
    (2025-09-03)
    Nanasombat, Suree
    ;
    Phunpruch, Saranya
    Fungal decay in carrots is a serious problem during postharvest storage. The present work aimed to study the antifungal effect of ammonium carbonate (AC) or potassium metabisulfite (PM) in combination with cinnamon or clove oils on delaying carrot decay caused by black rot mold during chill storage. In vitro trials were conducted to determine the antifungal activity of those acid salts, essential oils and their combinations against selected fungi isolated from carrots. Alternaria sp. C7D7 isolated from carrots was inhibited by AC and PM at 0.5-1.0% w/v minimum inhibitory concentration (MIC), while cinnamon and clove oils possessed strong anti-Alternaria effect with 0.025% w/v MIC. The combination of AC or PM at 0.25-0.5% w/v with cinnamon or clove oils at 0.04-0.25% w/v in potato dextrose agar produced a 100% antifungal index. Synergy testing by the checkerboard method showed that the combination of AC and cinnamon oil had partial synergistic effects against the mold. The optimum concentrations of combined AC or PM and cinnamon or clove oil were selected to formulate dipping solutions for carrot treatments. Their effect on controlling black rot decay in carrots during chill storage was investigated. The treatment with the dipping solutions consisting of combined 0.5% AC with 0.25% cinnamon oil or 1% AC alone caused a lower percentage of black rot decay (13.9-19.4% decay) in carrots compared to the others during storage at 5°C for 10 weeks. These findings show the usefulness of cinnamon oil and AC or their combination on delaying black rot decay in carrots.
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    Control of Aspergillus postharvest rot on plum tomato by potassium metabisulfite and potassium sorbate
    (2025-01-01)
    Nanasombat, Suree
    ;
    Phunpruch, Saranya
    In this study, fungal contamination of plum tomato fruits (Solanum lycopersicum) was preliminarily examined by the dilution plating method. The tomato samples were highly contaminated by molds and yeasts (1.0x10<sup>5</sup>-1.4x10<sup>8</sup> CFU/g), including Aspergillus, Geotrichum, Penicillium, Rhizopus, Candida, Cryptococcus and Rhodotorula. The mold strains that contaminated the tomatoes to the greatest extent were Penicillium and Rhizopus. Three fungal isolates (Aspergillus niger T6D5, Candida guilliermondii T7D4 and Rhodotorula mucilaginosa T7D5) were selected for further in vitro study. Then, the antifungal activity of organic acids (citric and tartaric acids) and salts (potassium acetate, potassium metabisulfite and potassium sorbate) against molds and yeasts was investigated. Salt solutions showed higher inhibitory effects than did acid solutions. Interestingly, A. niger T6D5 showed the highest susceptibility to potassium sorbate and potassium metabisulfite at 0.01-1% (w/v) MIC, respectively, compared to other fungi. In yeasts, potassium metabisulfite effectively inhibited the growth of C. guilliermondii T7D4 and R. mucilaginosa T7D5 at 0.25% (w/v) MIC. The use of organic acids and salts to control the growth of A. niger T6D5 on tomatoes was investigated. Among all the salts and acids tested, 1% (w/v) potassium metabisulfite and 3% (w/v) potassium sorbate completely inhibited the growth of A. niger T6D5 on tomatoes.