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Author: search.filters.author.Niemhom, NantawanSubject: search.filters.subject.Actinomycetes

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    Characterization, comparative genomics, and metabolome analysis of Streptomyces tuberiscabiei sp. nov., a novel actinomycete causing potato scab disease in Long Khot, Chiang Mai Province, Thailand
    (2026-07-01)
    Inthong, Tanchanok
    ;
    Wannawong, Thippawan
    ;
    Mhuantong, Wuttichai
    ;
    Hongprasit, Aphinan
    ;
    Tanasupawat, Somboon
    Three actinomycete strains, WSLK1–3<sup>T</sup>, WSLK1–4, and WSLK1–5, associated with scabby potato tuber, were characterized using polyphasic and genome-based taxonomy. All strains were found to be Gram-stain-positive, filamentous bacteria, including LL-diaminopimelic acid in cell-wall peptidoglycan. Whole-cell sugars were glucose, mannose, rhamnose, and ribose. MK-9(H<inf>6</inf>) and MK-9(H<inf>10</inf>) were major menaquinones; C<inf>16:0</inf>, isoC<inf>16:0</inf>, ante iso-C<inf>15:0</inf>, and anteiso -C<inf>17:0</inf> were major cellular fatty acids; diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, and phosphatidylinositol mannoside were major phospholipids; and DNA G + C contents were 71.5 mol%. Phylogenetic analysis based on 16S rRNA gene and genome sequences indicated that strains WSLK1–3<sup>T</sup>, WSLK1–4, and WSLK1–5 are closely related to Streptomyces canus DSM 40017<sup>T</sup> (=JCM 4212<sup>T</sup>), and S. pseudovenezuelae DSM 40212<sup>T</sup> (=JCM 11516<sup>T</sup>), respectively. The 16S rRNA gene sequences, average nucleotide identity based on BLAST (ANIb) and MUMmer (ANIm), and digital DNA-DNA hybridization (dDDH) values among the three strains were 100%, 99.12%, 99.33%, and 94.3%, respectively, indicating that these strains belong to the same species. Strains WSLK1–3<sup>T</sup>, WSLK1–4, and WSLK1–5 showed 88.54–90.62% ANIb, 90.99–92.27% ANIm, and 40.4–45.1% to their closely related type strains: S. canus JCM 4212<sup>T</sup>, and S. pseudovenezuelae JCM 11516<sup>T</sup>. All novel strains were pathogenic, causing necrosis on potato tuber slices, inhibiting plant seedlings, and inducing superficial to raised scab lesions on potato tubers. Based on the phenotypic, chemotaxonomic, pathogenic, and genomic data, strains WSLK1–3<sup>T</sup>, WSLK1–4, and WSLK1–5 could be assigned to the novel species within the genus Streptomyces for which the name Streptomyces tuberiscabiei sp. nov. is proposed. The type strain is WSLK1–3<sup>T</sup> (=TBRC 19150<sup>T</sup> = LMG 33893<sup>T</sup>).
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    Abyssomicin derivatives from the rhizosphere soil actinomycete Microbispora rhizosphaerae sp. nov. TBRC6028
    (2021-05-01)
    Bunbamrung, Nantiya
    ;
    Kittisrisopit, Suchada
    ;
    Intaraudom, Chakapong
    ;
    Dramae, Aibrohim
    ;
    Thawai, Chitti
    Three undescribed abyssomicin derivatives, including microbimisin, abyssomicins Z1, and Z2, were isolated from the soil actinomycete Microbispora rhizosphaerae sp. nov. TBRC6028. Chemical structures were determined by NMR spectroscopic data (<sup>1</sup>H, <sup>13</sup>C, COSY, HSQC, HMBC, and NOESY spectra) and the absolute configurations were verified by single-crystal X-ray diffraction analyses together with the ECD spectral data. Microbimisin and abyssomicin Z1 exhibited weak antibacterial activity against Bacillus cereus with MIC values of 25.0 and 50.0 μg/mL without cytotoxicity against MCF-7 and Vero cells at the concentration of 50 μg/mL.
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    Herbidospora soli sp. Nov., isolated from soil
    (2018-01-01)
    Niemhom, Nantawan
    ;
    Thawai, Chitti
    A novel actinomycete strain PS42-9<sup>T</sup>, which formed short chains of spores borne on the tip of long sporophores arising from the substrate mycelium, was isolated from soil in Phu-Sang National Park, Phayao province, Thailand. The isolate contained meso-diaminopimelic acid in the cell-wall peptidoglycan. The whole-cell sugars of strain PS42-9<sup>T</sup> were glucose, madurose, mannose, rhamnose and ribose. The characteristic phospholipids were phosphatidylethanolamine, phosphatidylmethylethanolamine, hydroxyphosphatidylethanolamine and ninhydrin-positive glycophospholipids. The major menaquinone was MK-10(H<inf>4</inf>). The main cellular fatty acids were C<inf>17:</inf>1ω8c and C<inf>17: 0</inf>. The G+C content of the genomic DNA was 71.5 mol%. Phylogenetic analysis using 16S rRNA gene sequences revealed that strain PS42-9<sup>T</sup> should be classified in the genus Herbidospora and was closely related to Herbidospora sakaeratensis DMKUA 205<sup>T</sup> (99.10 %) and Herbidospora yilanensis NBRC 106371<sup>T</sup> (98.61 %). The result of DNA–DNA hybridization and some physiological and biochemical properties indicated that strain PS42-9<sup>T</sup> could be readily distinguished from its closest phylogenetic relatives. On the basis of these phenotypic and genotypic data, this strain represents a novel species, for which the name Herbidospora soli sp. nov. is proposed. The type strain is PS42-9<sup>T</sup> (=BCC 46909<sup>T</sup>=NBRC 108780<sup>T</sup>).