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Study on the regeneration of sausage tree (Kigelia africana) by tissue culture
(2026-05-01)
Poeaim, S.
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Poeaim, A.
This study investigated the regeneration of sausage tree (Kigelia africana) using tissue culture techniques, focusing on callus induction, shoot regeneration from seeds, and root induction from shoot explants. Seeds were cultured on solid MS medium supplemented with various concentrations of BA (0.5, 1, 2, 3, and 5 mg L⁻¹) for 10 weeks. Callus formation was observed at all BA concentrations, with the largest mean callus volume (5214.17 mm³) and highest induction rate (80%) achieved at 5 mg L⁻¹. Shoot regeneration occurred across all BA treatments, with the highest number of shoots (45 shoots; 86.54%) obtained on medium containing 1 mg L⁻¹ BA. Root induction was studied using shoot segments derived from seed-induced shoots cultured on MS medium supplemented with IAA, NAA, or IBA for 4 weeks. IAA at 0.5 mg L⁻¹ induced roots in 33.33% of explants with an average root length of 6.68 mm. NAA at 2 mg L⁻¹ promoted the highest root induction (70.83%), though roots were shorter, while 1 mg L⁻¹ NAA produced fewer roots but with greater length (7.00 mm). IBA at 3 mg L⁻¹ induced rooting in 33.33% of explants with an average root length of 3.78 mm and also stimulated callus formation, with the largest callus volume observed at 2 mg L⁻¹. These results demonstrate that BA is effective for callus and shoot induction, whereas IAA, NAA, and IBA promote root formation, with distinct responses at different concentrations. The optimized protocols provide a foundation for in vitro propagation, mass multiplication, and conservation of K. africana, with potential applications in biotechnology and secondary metabolite production.
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Drought tolerance of Stylosanthes guianensis CIAT 184 by tissue culture
(2026-05-01)
Poeaim, S.
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Ngoenngam, L.
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Poeaim, A.
The multiple shoots induction of Stylosanthes guianensis CIAT 184 was cultured on MS medium supplemented with 3 mg/l TDZ. The percentage of shoot induction was 100%, the average number of shoots was 36.80 per seed, and the average height of shoots was 0.76 cm. The shoots were transferred to MS medium for rooting, supplemented with 0.3 mg/l IAA. The percentage of root induction was 40%, and the average number of roots was 6.50 per shoot. Physical gamma rays induced the mutation. Seeds were irradiated with 0, 5, 10, 15, 20, 25, 30 and 50 Krad. The results showed that S. guianensis CIAT 184 irradiated at 44.38 Krad gave 50 % (GR50(30)) and 50% LD50(30) of 38.06 Krad. The drought tolerance of seeds supplemented with 0, 5, 10, 15 and 20% of PEG-6000. The GR50 was 17.73 % at 2 weeks, and the LD50 was 14.86 % and 11.83 % for 4 and 12 weeks, respectively.
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Plant regeneration of Dimocarpus scandens (Winit & Kerr) Boonsuk & Chantar. (Lamyai-Thao) by tissue culture technique
(2025-05-01)
Jitwetkul, A.
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Poeaim, A.
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Pongtongkam, P.
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Chareonsap, P. P.
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Poeaim, S.
The minimum contamination percentage (25%) was recorded in the experiment using 0.2% mercuric chloride (HgCl2) for 20 min, which was observed to be a 50% germination rate after 2 weeks of culture. The maximum germination rate (68%) was obtained on Murashige and Skoog (MS) solid medium supplemented with 1.0 mg/L 6-benzylaminopurine (BAP), giving a shoot length of 48.00 mm. and root length of 31.40 mm. The optimal condition of nodal segment sterilization was recorded in the experiment using 0.1% HgCl2 for 10 min, which gave 80% survival explants. For shoot induction, it was worked on MS solid medium with 0.5 mg/L 6-benzylaminopurine (BAP) that induced the highest average length of 11.31 mm. The highest root formation frequency (50%) and root length (3.0 mm) were achieved on an MS medium containing 2.0 mg/L indole-3-butyric acid (IBA). The plantlets were acclimatized and established in the soil.
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Plant regeneration of Bauhinia purpurea by tissue culture technique
(2024-05-01)
Saparam, W.
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Poeaim, A.
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Pongtongkam, P.
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Chareonsap, P. P.
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Poeaim, S.
Results showed that the seed of Bauhinia purpurea germination was archived 100% from half-strength MS medium supplemented with 3.0 mg/L Gibberellic acids (GA3) and gave 71.137 mm for the highest seedling. The optimal condition of nodal explants sterilization was E5, which gave 92% of survival explants. For the shoot induction method, the results showed that 24.270 mm was the highest proliferation of shoots achieved from MS medium supplemented with 1.0 mg/L 6-benzylaminopurine (BAP). The highest number of roots was 2.867 roots, and the highest root length was 33.453 mm, obtained at half-strength MS medium complemented with 0.25 mg/L Indole-3-acetic acid (IAA). The 73.33% of plantlets were survived after being transferred to plastic pot.
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Effects of growth regulators on in vitro propagation of Sophora tomentosa L. (Necklace pod)
(2024-05-01)
Rungprateeppaiboon, N.
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Chareonsap, P. P.
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Pongtongkam, P.
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Poeaim, A.
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Poeaim, S.
Seeds of Sophora tomentosa were sterilized and germinated on MS medium with BAP (1 mg/L), which was observed to be 75% germination rate with the highest amount of multiple shoots (3 shoots per seed) and an average length of 29.56 mm. The germination rate was reduced to 33.33% by 1% NaCl. Multiple shoot induction was worked on MS medium with 2.0 mg/L of TDZ that induced the highest shoot bud formation rate (46.67%) with the highest amount of shoot bud (10.71 buds per culture), while 1.0 mg/L GA3 showed the highest average length of 34.13 mm. For root induction, 20% rooting was formed in 1/2 MS medium with a combination of 0.5 mg/L IAA and BAP with 0.1% AC, with 3 roots per culture and an average root length of 15.36 mm. After acclimatization for 16 weeks, 70% of the plantlets successfully survived.
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Study on optimization for in vitro propagation of Dalbergia oliveri by plant tissue culture
(2024-03-01)
Nonthasila, S.
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Pongtongkam, P.
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Chareonsap, P. P.
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Poeaim, A.
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Poeaim, S.
The experimental findings demonstrated that the highest proliferation frequency (100%), along with the maximum number of shoots per explant (3.33±0.52 shoots) and an average shoot length of 30.04±1.70 mm, were achieved in the Murashige and Skoog (MS) medium supplemented with 2.0 mg/l of 6-benzylaminopurine (BAP). Subsequently, upon reaching a height of 3 to 4 cm, the in vitro micro shoots were transferred to a ¼ MS medium enriched with 0.75 mg/l of indole-3-acetic acid (IAA). This particular regimen resulted in the highest number of roots per shootlet (3.50±0.58) with an average root length of 34.19±0.79 mm. The acclimatization phase of the plantlets to the soil was successful, demonstrating a commendable survival rate of 80%.
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Micropropagation of Ironwood (Xylia xylocarpa (Roxb.) Taub.) by tissue culture
(2024-03-01)
Panudom, S.
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Pongtongkam, P.
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Chareonsap, P. P.
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Poeaim, A.
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Poeaim, S.
It was found that the sterilization procedure was highly effective, resulting in a 100% survival rate of Xylia xylocarpa (Roxb.). After sterilization, the seeds were cultured on a half-strength Murashige and Skoog (MS) medium without adding plant growth regulators (PGRs). A maximum germination percentage of 100% was achieved after 2 weeks of culture. 1.5 cm-sized node segments from both seeds and mother trees were used for shoot induction. These node segments were treated with the same sterilization conditions as the seeds but with the addition of 0.1% HgCl2. The node segments achieved a 90% survival rate despite the additional chemicals. The node segments from seeds were then cultured on a full-strength MS medium supplemented with 0.50 mg/l of BAP. The results showed a maximum number of shoots, reaching 4.10±1.20 per explant and a mean shoot length of 3.87±0.30 cm. The part of node segments derived from mother trees showed 3.50±1.27 shoots per explant, accompanied by a mean shoot length of 3.18±0.89 cm when using BAP concentration of 0.25 mg/l after 12 weeks of cultivation. The rooting medium contained half-strength MS medium supplemented with 0.75 mg/l of IBA. After 8 weeks of being cultured in the rooting medium, the shoots exhibited a maximum rooting percentage of about 80%. The technique provides a valuable method for rapidly propagating and conserving this plant species in a controlled and efficient manner.
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Assessing genetic diversity of Yellow Star tree using SRAP markers and efficacy of their endophytic fungi in biological control
(2023-05-01)
Poeaim, S.
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Tangthirasunun, N.
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Chareonsap, P. P.
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Poeaim, A.
The result showed that 52 fragments (64.22%) were polymorphic bands from seven combinations of Sequence-Related Amplified Polymorphism (SRAP) markers. The genetic relationship was analyzed using the UPGMA method by NTSYpc software version 2.11X showed a similarity coefficient ranging from 0.59 to 0.98. Endophytic fungi were isolated from fresh leaves of Yellow Star (Schoutenia glomerata King subsp. peregrina (Craib) Roekm. & Hartono and identified by morphology and DNA barcoding. The fungi were identified in Alternaria alternata, Colletotrichum siamense, Diaporthe arengae, Phyllosticta capitalensis, and Xylaria cubensis. The dual culture test evaluated the efficacy of endophytic fungi isolated from Yellow Star tree against Colletotrichum gloeosporioides and Phomopsis asparagi causing disease in asparagus. The results revealed that X. cubensis had the potential to inhibit the spore germination of the C. gloeosporioides ASGC06 and P. asparagi ASGP04, which inhibited spore germination of 99.97 and 100 percent, respectively. However, this fungus should be further studied for biological control and bioactive secondary metabolites.
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Phytochemical screening, antioxidant activity and total phenolic content of methanolic extract of Phak Wan Ton (Crotalaria medicaginea Lam.)
(2023-01-01)
Thuion, T.
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Poeaim, S.
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Poeaim, A.
The chlorophyll content of the methanolic extract of Crotalaria medicaginea without chlorophyll removal ranged from 0.50±0.03 to 1.24±0.06 mg/g extract, and with chlorophyll removal ranged from 0.09±0.01 to 0.18±0.00 mg/g extract. The highest total phenolic content using the Folin-Ciocalteu method was presented in the leaves extract without chlorophyll removal as 48.33±0.51 mg GEA/g extract. Moreover, the leaves extract without chlorophyll removal showed the lowest antioxidant activity with 50% inhibitory concentration (IC<inf>50</inf>) in DPPH radical scavenging assay at 344.76 µg/ml. Besides, the root extract exhibited rich potential for gram-positive bacteria, including Bacillus cereus, Bacillus subtilis, Kocuria rhizophila, Staphylococcus aureus and Staphylococcus epidermidis. The overall results showed that the extracts without chlorophyll removal had more efficiency than those with chlorophyll removal. Hence, the phytochemical screening was investigated only in the methanolic extract without chlorophyll removal. All extracts found alkaloids and coumarins, while tannins were found only in roots and did not display saponins. The current study suggested that the extracts of C. medicaginea Lam. gave the potential source of natural bioactive compounds.
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A new method to increase plant quantity through tissue culture on Vitex negundo L
(2022-09-01)
Poeaim, A.
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Prasertkittikul, N.
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Wangsor, N.
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Boonmee, W.
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Laipasu, P.
A new method to increase plant quantity through tissue culture on Vitex negundo L. was discovered. The influences of PGRs on shoot regeneration cultured on MS medium with 0.5, 1, 2, or 3 mg/L benzylaminopurine (BAP), meta-Topolin (mT) and Thidiazuron (TDZ) were resulted to be the greatest percentage of shoot development was 100% at 1 mg/L BAP in MS medium. The greatest average number of shoot multiplication was 4.40 shoots at 0.5 mg/L BAP in MS medium after 8 weeks of culture. For roots, the result of MS media fortified with 0.5, 1, 2, and 3 mg/L of indole 3 butyric acid (IBA) and naphthalene acetic acid (NAA) discovered that the excellent percentage of root production was 90% at 3 mg/L IBA. The highest average number of root production was 108.20 roots on 2 mg/L NAA after 8 weeks of culture. The plantlets were eventually transferred into the plastic pots. In this research work, the nodes were demonstrated to be an outstanding regenerating explant resource for Vitex negundo L. An effective regeneration strategy for plantlets derived from the nodes was demonstrated.

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