KMITL

Permanent URI for this communityhttps://dspace.kmitl.ac.th/handle/123456789/1

Browse

Settings

Author: search.filters.author.Saetiew, K.Subject: search.filters.subject.Callus induction

Search Results

Now showing 1 - 2 of 2
  • Loading...
    Thumbnail Image
    Some of the metrics are blocked by your 
    consent settings
    Item type:Publication,
    Influence of plant growth regulators on shoot development of Chrysanthemums
    (2024-01-01)
    Boonkamjat, T.
    ;
    Saetiew, K.
    ;
    Teerarak, M.
    Plant nodes were cultured on MS mediums supplemented with multiple concentrations of plant growth regulators. Sunny Snow produced the largest callus of 1.19×1.19 cm and obtained 93.33% of new shoots with 10 . 0 0 shoots per callus, and 0. 84 g callus fresh weight, when grew in MS medium supplemented with 1 mg/l BA + 0.1 mg/l IAA as compared to the control which no callus was produced. After 20 weeks of culturing, the second set showed that the control treatment of Sunny Snow gave the highest stem growth and the best canopy size of 9.00 and 5.60 cm respectively. Chompoo Phan gave the highest scores of chlorophyll content, i.e., chl a 652.14 µg/g FW, chl b 332.47 µg/g FW and carotenoid 165.92 µg/g FW. Besides, MDA appeared to be highest, 17.37 nmol/g FW when cultured on MS medium supplemented with 1 mg/l BA + 0.1 mg/l IAA.
  • Loading...
    Thumbnail Image
    Some of the metrics are blocked by your 
    consent settings
    Item type:Publication,
    Callus and hairy root induction of Melaleuca cajuputi Powell
    (2020-05-01)
    Prangthong, U.
    ;
    Montri, N.
    ;
    Saetiew, K.
    It is stated that the lowest percentage of contamination at 49.63% and highest percentage of survival at 37.95% after sterilized with 70% ethanol for 1 min and 1.8 % NaOCl for 30 min. The bud explants were cultured on Murashige and Skoog (MS) medium supplemented with 0, 0.02, 0.04, 0.06, 0.08 mg/l 2,4-Dinitrophenylhylhydrazine (2,4-D) combination with 0, 0.02, 0.04, 0.06, and 0.08 mg/l Thidiazuron (TDZ) for callus induction. The results showed that the explants were cultured on MS medium supplemented with 0.06 mg/l TDZ resulting to be suitable for callus induction with biggest size at 65.2x1. 22cm of width and length. Culturing buds on MS medium supplemented with 0.02 mg/l TDZ had the highest averaged shoot number. The roots were cultured in liquid MS medium supplemented with 0.04 mg/l TDZ, 0.04 mg/l 2.4-D gave the highest weight of hairy roots.