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Factors affecting hydrogen production by unicellular green alga chlamydomonas reinhardtii cc-125
(2021-01-01)
Sereetrakul, Kodchaporn
;
Phunpruch, Saranya
In this study, factors affecting H<inf>2</inf> production by the unicellular green alga Chlamydomonas reinhardtii CC-125 were investigated. It was found that the cell density, O<inf>2</inf> concentration and nutrient deprivation in media play important roles in H<inf>2</inf> production by C. reinhardtii CC-125. C. reinhardtii CC-125 at a cell age of 36 hours with an optical density at 750 nm of 0.8 shows the highest H<inf>2</inf> production rate. Similar to other microorganisms, higher O<inf>2</inf> concentrations decrease H<inf>2</inf> production. However, hydrogenase activity in C. reinhardtii CC-125 seems to show high O<inf>2</inf> tolerance, with an O<inf>2</inf>I<inf>50</inf> of 16.87 ± 0.81%. Under atmospheric air, maximum H<inf>2</inf> production of 100.90 ± 7.92 and 107.47 ± 3.72 mL L<sup>-1</sup> was found in cells incubated in sulfur-deprived and sulfur-nitrogen-deprived media, respectively, after 7 days of incubation. The deprivation of both sulfur and nitrogen prolongs H<inf>2</inf> production in this algal strain.
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