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Streptomyces telluris sp. nov., a promising terrestrial actinobacterium with antioxidative potentials
(2023-06-01)
Thayanuwadtanawong, Onnicha
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Duangupama, Thitikorn
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Bunbamrung, Nantiya
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Pittayakhajonwut, Pattama
;
Intaraudom, Chakapong
An actinomycete strain, AA8T, which produced a long straight chain of spores (verticillati type), was isolated from the rhizosphere soil of Mangifera indica in Bangkok, Thailand. A polyphasic taxonomic study was carried out to establish the taxonomic position of the strain. Strain AA8T formed a tight taxonomic position in the 16S rRNA gene tree with Streptomyces roseifaciens MBT76T. In contrast, the genome-based taxonomic analysis showed that strain AA8T shared low average nucleotide identity-BLAST (94.1%), the digital DNA–DNA hybridization (58.2%), and the average amino acid identity (93.6%) values with S. roseifaciens MBT76T. Moreover, a combination of physiological and biochemical properties indicated that strain AA8T was distinguished from all Streptomyces species with effectively published names. Strain AA8T, therefore, represents a novel species of Streptomyces, and the name Streptomyces telluris is proposed for the strain. The type strain is AA8T (= TBRC 8483T = NBRC 113461T). The chemical investigation led to the isolation of nine known compounds (compounds 1–9). Among these compounds, compound 7 (3,4-dihydroxybenzaldehyde) possesses strong antioxidant activity equal to ascorbic acid, a powerful antioxidative agent.
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Abyssomicin derivatives from the rhizosphere soil actinomycete Microbispora rhizosphaerae sp. nov. TBRC6028
(2021-05-01)
Bunbamrung, Nantiya
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Kittisrisopit, Suchada
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Intaraudom, Chakapong
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Dramae, Aibrohim
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Thawai, Chitti
Three undescribed abyssomicin derivatives, including microbimisin, abyssomicins Z1, and Z2, were isolated from the soil actinomycete Microbispora rhizosphaerae sp. nov. TBRC6028. Chemical structures were determined by NMR spectroscopic data (1H, 13C, COSY, HSQC, HMBC, and NOESY spectra) and the absolute configurations were verified by single-crystal X-ray diffraction analyses together with the ECD spectral data. Microbimisin and abyssomicin Z1 exhibited weak antibacterial activity against Bacillus cereus with MIC values of 25.0 and 50.0 μg/mL without cytotoxicity against MCF-7 and Vero cells at the concentration of 50 μg/mL.
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Streptomyces musisoli sp. nov., an actinomycete isolated from soil
(2021-01-01)
Duangupama, Thitikorn
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Intaraudom, Chakapong
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Pittayakhajonwut, Pattama
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Suriyachadkun, Chanwit
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Tadtong, Sarin
An actinobacterium, strain CH5-8T, which formed spiral chains of spore arising from the aerial mycelium, was isolated from rhizosphere soil of Musa spp. The organism exhibited vivid greenish yellow substrate mycelium and easily produced the medium grey aerial spore mass on ISP2 medium. The typical chemotaxonomic properties of members of the genus Streptomyces were observed for strain CH5-8T, e.g. ll-diaminopimelic acid in cell peptidoglycan, MK-9(H<inf>8</inf>), MK-9(H<inf>6</inf>), and MK-9(H<inf>4</inf>) as major menaquinones and anteiso-C<inf>15:0</inf>, iso-C<inf>16:0</inf>, and anteiso-C<inf>17:0</inf> as major fatty acids. Diphosphatidylglycerol, phosphatidylethanola-mine, hydroxyphosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannoside were detected in the cells. A combination of morphological and chemotaxonomic data supported the assignment to the genus Strep-tomyces. The analysis result obtained for the 16S rRNA gene sequence confirmed the taxonomic affiliation at the genus level of this strain. The novel strain CH5-8T showed the highest 16S rRNA gene sequence values to Streptomyces echinatus NBRC 12763T (98.9%), followed by Streptomyces actinomycinicus RCU-197T (98.9%). The average nucleotide identity by blast (ANIb) and digital DNA–DNA hybridization values between CH5-8T and its closest relatives, S. echinatus CECT 3313T and S. actinomycinicus RCU-197T, were ≤91.6% and ≤47.4%, respectively. The digital DNA G+C content of genomic DNA was 72.1 mol%. On the basis of these phenotypic and genotypic data, strain CH5-8T represents a novel species, for which the name Streptomyces musisoli sp. nov. is proposed. The type strain is CH5-8T (=TBRC 9950T=NBRC 113997T).
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Antibacterial, antitubercular, antimalarial and cytotoxic substances from the endophytic Streptomyces sp. TBRC7642
(2020-04-01)
Bunbamrung, Nantiya
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Intaraudom, Chakapong
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Dramae, Aibrohim
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Thawai, Chitti
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Tadtong, Sarin
Eight previously undescribed naturally-occurring compounds, including abyssomycins Y - Z, methyl aeruginoate, desferri-ferrithocin-4-hydroxyphenethylester, streptomethiocins A - B, furaquinocin I, and streptolactone, along with eleven known compounds were isolated from the endophytic Streptomyces sp. TBRC7642. The chemical structures were determined based on spectroscopic means including 1D, 2D NMR spectroscopy and mass spectrometry. The absolute configurations were assigned by relying on CD spectra and their optical rotations. In addition, the isolated compounds were evaluated for biological activity, such as antimalarial, antitubercular, antibacterial (both Gram-positive and Gram-negative bacteria), as well as for cytotoxicity against MCF-7, NCI–H187, and Vero cells.
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Micromonospora caldifontis sp. nov., isolated from hot spring soil
(2019-05-01)
Thawai, Chitti
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Tanasupawat, Somboon
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Kudo, Takuji
A single spore forming actinomycete, designated strain HSS6-8T, was isolated from a sample of hot spring soil. The strain had the chemotaxonomic properties consistent with its classification in the genus Micromonospora. The strain was found to have meso-diaminopimelic acid in the cell-wall peptidoglycan. The acyl type of the cell-wall muramic acid was glycolyl. The reducing sugars in the cell hydrolysates were glucose, arabinose, xylose, ribose, mannose, galactose and rhamnose. The phospholipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and phosphoglycolipid. The major menaquinones were MK-10(H<inf>6</inf>) and MK-10(H<inf>4</inf>). The major cellular fatty acids were iso-C<inf>16: 0</inf>, anteiso-C<inf>17: 0</inf>, C<inf>17: 0 </inf>and anteiso-C<inf>15: 0</inf>. The G+C content of the genomic DNA was 70.5 mol%. 16S rRNA gene sequence analysis revealed that strain HSS6-8T was closely related to Micromonospora nigra DSM 43818T (98.2%), Micromonospora eburnea DSM 44814T (98.2%) and Micromonospora spongicola S3-1T (98.1%). The physiological and DNA-DNA hybridization data allowed the differentiation of strain HSS6-8T from its related species. Thus, the strain represents a novel species of the genus Micromonospora, for which the name Micromonospora caldifontis sp. nov. is proposed. The type strain is HSS6-8T (=TBRC 8927T=JCM 17126T).
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Thermocatellispora soli sp. nov., isolated from hot spring soil
(2019-01-01)
Duangupama, Thitikorn
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Pittayakhajonwut, Pattama
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Thawai, Chitti
An aerobic, spore-forming, actinomycete, designated strain CHM3-46 T , was isolated from soil in a hot spring pond located in Chiangmai province, Thailand. The strain exhibited taxonomic characteristics consistent with the genus Thermocatellispora. Strain CHM3-46 T produced short, straight chains of warty spores on aerial mycelia. The presence of meso-diaminopimelic acid was observed in the cell-wall peptidoglycan. The whole-cell reducing sugars were glucose, mannose, galacose and ribose. The phospholipids comprised phosphatidylmethylethanolamine, hydroxyphosphatidylmethylethanolamine, phosphatidylethanolamine, hydroxyphosphatidylethanolamine, diphosphatidylglycerol, phosphatidylinositol, four phosphoglycolipids and three unidentified phospholipids. The predominant menaquinones were MK-9(H <inf>4</inf> ), MK-9(H <inf>6</inf> ) and MK-9 (H <inf>8</inf> ). 10-methyl C <inf>17 : 0</inf> , C <inf>16 : 0</inf> , C <inf>17 : 0</inf> and iso-C <inf>16 : 0</inf> were identified as the main cellular fatty acids. The G+C content of the genomic DNA was 73.2 mol%. Analysis of the 16S rRNA gene sequence showed that strain CHM3-46T belonged to the genus Thermocatellispora, exhibiting the highest similarity to Thermocatellispora tengchongensis YIM 77521 T (98.5 %). Furthermore, a low DNA relatedness value (23.4 %±0.8) and several physiological and biochemical characteristic differences were detected between strain CHM3-46 T and its closest relative. Based on the taxonomic data, strain CHM3-46 T could be readily distinguished from its closest phylogenetic relative and represents a novel species, for which the name Thermocatellispora soli sp. nov. is proposed. The type strain is CHM3-46 T (=TBRC 7649 T =NBRC 113148 T ).
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Microbispora soli sp. nov., isolated from soil of a hot spring
(2018-12-01)
Kittisrisopit, Suchada
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Pittayakhajonwut, Pattama
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Tadtong, Sarin
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Thawai, Chitti
A novel actinomycete, strain RTBAU4-9 T , which produced longitudinally paired spores on aerial mycelia, was isolated from a soil sample of a hot spring area. This strain exhibited chemotaxonomic and genotypic properties typical of the genus Microbispora, such as meso-diaminopimelic acid in the cell-wall peptidoglycan, glucose, ribose and trace amount of madurose as the characteristic whole-cell sugars, phosphatidylethanolamine, diphosphatidylglycerol and phosphoglycolipids as the membrane phospholipids, MK-9(H <inf>4</inf> ), MK-9(H <inf>2</inf> ) and MK-9(H <inf>0</inf> ) as the characteristic menaquinones, and iso-C <inf>16: 0</inf> and anteiso-C <inf>17: 0</inf> as the main fatty acids. The G+C content of the genomic DNA was 70.2 mol%. The result of 16S rRNA gene sequence analysis revealed the strain was a member of the genus Microbispora and was most closely related to Microbispora hainanensis 211020 T (98.3% 16S rRNA gene sequence similarity). In addition, the low percentage of DNA-DNA relatedness (<28.1±1.2 %) and several phenotypic differences confirmed that strain RTBAU4-9 T should be considered as representing a novel species of the genus Microbispora, for which the name Microbispora soli sp. nov. is proposed. The type strain is RTBAU4-9 T (=TBRC 7648 T =NBRC 113147 T ).
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Jishengella zingiberis sp. Nov., isolated from root tissue of zingiber montanum
(2018-10-01)
Thawai, Chitti
;
He, Ya Wen
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Tadtong, Sarin
A novel actinomycete, strain PLAI 1-1T, which formed spiny single spore directly on substrate mycelium was isolated from root tissue of Zingiber montanum. The isolate contained meso-diaminopimelic acid and 3-hydroxydiaminopimelic acid in the cell-wall peptidoglycan. The acyl type of the cell-wall muramic acid was glycolyl. The whole-cell sugars of strain PLAI 1-1T were glucose, arabinose, xylose, ribose and a trace amount of mannose. The membrane phospholipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylserine and phosphatidylinositol. The major menaquinone was MK-9 (H<inf>4</inf>). The main cellular fatty acids were iso-C<inf>15: 0</inf> and C<inf>17: 1</inf> w8c. The G+C content of the genomic DNA was 70.6 mol%. 16S rRNA gene sequence analysis revealed that strain PLAI 1-1T was a member of the genus Jishengella and had the highest 16S rRNA gene sequence similarity to Jishengella endophytica DSM 45430T (99.2 %). Based on the data of physiological and biochemical tests, including the result of DNA–DNA hybridization, strain PLAI 1-1T represents a novel species of the genus Jishengella, for which the name Jishengella zingiberis sp. nov. is proposed. The type strain is PLAI 1-1T (=TBRC 7644T =NBRC 113144T ).
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Amycolatopsis rhizosphaerae sp. Nov., isolated from rice rhizosphere soil
(2018-05-01)
Thawai, Chitti
A novel actinomycete, designated strain DH51B-4-3T, which formed long chains of spherical spores borne on the tip of sporophores arising from the aerial mycelium, was isolated from rice rhizosphere soil. The isolate contained meso-diaminopimelic acid in the cell-wall peptidoglycan. The whole-cell sugars of strain DH51B-4-3T were arabinose, galactose, glucose, rhamnose and ribose. The phospholipids in the cell membrane were phosphatidylethanolamine, phosphatidyl methylethanolamine, diphosphatidylglycerol and phosphatidylinositol. The major menaquinone was MK-9(H<inf>4</inf>). The main cellular fatty acids were iso-C<inf>16:0</inf> and cyclo-C<inf>17:0</inf>. The G+Ccontent of the genomic DNA was 68.2mol%. Phylogenetic analysis using 16S rRNA gene sequences revealed that strain DH51B-4-3T should be classified in the genus Amycolatopsis and closely related to Amycolatopsis dongchuanensis YIM 75904T (98.06%) and Amycolatopsis sacchari DSM 44468T (97.77%). The result of DNA-DNA hybridization and some physiological and biochemical properties indicated that strain DH51B-4-3T could be readily distinguished from its closest phylogenetic relatives. On the basis of these phenotypic and genotypic data, this strain represents a novel species, for which the name Amycolatopsis rhizosphaerae sp. nov. is proposed. The type strain is DH51B-4-3T (=TBRC 6029T=NBRC 112509T).
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Pseudonocardia soli sp. nov., isolated from mountain soil
(2018-04-01)
Thawai, Chitti
A novel actinomycete, designated strain NW8-21T, was isolated from mountain soil in Mae-Wong National Park, Nakornsawan province, Thailand, and was taxonomically characterized by using a polyphasic approach. Based on 16S rRNA gene sequence analysis, the strain was revealed to have the closest similarity to Pseudonocardia endophytica YIM 56035T with the highest 16S rRNA gene sequence similarity value of 98.7 %, followed by Pseudonocardia nantongensis KLBMP 1282T (98.0 %). The chemotaxonomic properties, i.e. arabinose and galactose as the diagnostic reducing sugar in cells, MK-8 (H<inf>4</inf>) as a major menaquinone, iso-C<inf>16:0</inf> as the main cellular fatty acid component and phosphatidylethanolamine, phosphatidylmethylethanolamine, diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol, phosphatidylcholine as the characteristic phospholipids, confirmed a taxonomic affiliation of the strain that was consistent with those of the genus Pseudonocardia. Several phenotypic differences and the DNA-DNA hybridization results (less than 40% relatedness value) indicated that strain NW8-21T shoud be considered to represent a novel species of the genus Pseudonocardia, for which the name Pseudonocardia soli is proposed. The type strain is strain NW8-21T (=BCC 58125T=NBRC 109519T).

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