KMITL

Permanent URI for this communityhttps://dspace.kmitl.ac.th/handle/123456789/1

Browse

Settings

Start date: 2021Subject: search.filters.subject.Carotenoids

Search Results

Now showing 1 - 4 of 4
  • Loading...
    Thumbnail Image
    Some of the metrics are blocked by your 
    consent settings
    Item type:Publication,
    Enhanced carotenoid production in Chlorococcum humicola via two-stage cultivation in synthetic wastewater under white light stress conditions
    (2026-12-01)
    Umpuch, Chakkrit
    ;
    Krommuang, Atchari
    ;
    Paopo, Idtisak
    ;
    Kunyawut, Chatchai
    Carotenoids are high-value pigments widely used in nutraceutical and pharmaceutical applications due to their antioxidant properties. This study investigates the effect of white light intensity on carotenoid biosynthesis in Chlorococcum humicola cultivated in synthetic domestic wastewater using a two-stage process in a 10-L airlift photobioreactor. The first stage (green phase) supported optimal biomass growth at 3,500 Lux, followed by a stress-induced red phase under nitrogen depletion with elevated light intensities ranging from 3,500 to 60,000 Lux. Biomass, chlorophyll, fatty acids, and carotenoids were analyzed, and β-carotene was identified using HPLC. At 20,000–40,000 Lux, cells preferentially accumulated fatty acids up to 26.69 ±0.14 percent of dry weight, indicating a metabolic shift toward energy storage. Exposure to 60,000 Lux significantly enhanced carotenoid production, reaching 1.69 ± 0.03 mg L<sup>−1</sup>, with β-carotene accounting for 17.16 ± 0.04 percent of total carotenoids. Statistical analysis confirmed significant differences among treatments (p < 0.05). The stable chlorophyll-to-biomass ratio suggests metabolic reallocation rather than pigment degradation. These findings demonstrate the photoadaptive capacity of C. humicola and highlight white light as an eco-friendly strategy for enhancing carotenoid production in scalable systems.
  • Loading...
    Thumbnail Image
    Some of the metrics are blocked by your 
    consent settings
    Item type:Publication,
    Influence of Gac aril, yeast, and sugar in high quality sandwich bread making
    (2023-05-01)
    Thuy, Nguyen Minh
    ;
    Xuyen, Ho Bui Bao
    ;
    Thanh, Nguyen Van
    ;
    Giau, Tran Ngoc
    ;
    Tien, Vo Quoc
    Research was conducted to develop a sandwich bread with added Gac aril. Effects of Gac aril (5, 7, and 9%), added sugar content (8.6, 10.3, and 12%), and instant dry yeast used (0.3, 0.4, and 0.5%) to bread quality and sensory value of the products were performed. The relationship between the dependent variables such as β-carotene, lycopene, and volume expansion and three independent variables including Gac aril, sugar and dry yeast content was analyzed using multiple regression analysis. It was observed that the optimum responses of β-carotene, lycopene, and volume expansion were determined as 14.78 µg/g, 59.14 µg/g, and 5.3 times, respectively. To achieve those optimal values, the optimization conditions of various input variables, the percentage of Gac aril, sugar, and dried yeast were found to be 8.55%, 9.76%, and 0.5%, respectively, which, on confirmation, showed a high quality sandwich bread. The optimal parameters were also verified. Sensory evaluation showed that the sandwich bread prepared with 8.55% and 9% Gac aril gave a higher acceptance score than the lesser content and control sample. Preference mapping also showed more than 80% of the participants loved these products.
  • Loading...
    Thumbnail Image
    Some of the metrics are blocked by your 
    consent settings
    Item type:Publication,
    Identification of major carotenoids from green alga Tetraspora sp. CU2551: partial purification and characterization of lutein, canthaxanthin, neochrome, and β-carotene
    (2022-08-01)
    Maswanna, Thanaporn
    ;
    Maneeruttanarungroj, Cherdsak
    The green algae Tetraspora sp. CU2551 was previously identified as a strain with high potential for biohydrogen production; however, its algal biomass characteristics changed from green to reddish orange within 43 days of biohydrogen production. The crude pigments were extracted, partially purified, and characterized by chemical determination. The present study focused on elucidating the carotenoid composition of the selected green alga Tetraspora sp. CU2551. The pigment extract was partially purified and fractionated using thin layer chromatography, and yielded two major and two minor carotenoid bands. The fractions were confirmed by high-performance liquid chromatography with a diode array detector (HPLC–DAD) before being identified and confirmed using Liquid Chromatograph-Quadrupole Time of Flight-Mass Spectrometry (LC-QTOF-MS). The spectral data of these fractions revealed four sub-fractions of interest that were lutein, canthaxanthin, neochrome, and β-carotene, which had percentages in the crude extracts of 30.57%, 25.47%, 7.89%, and 0.71%, respectively. Lutein and canthaxanthin were found to be the major carotenoid pigments present. Our findings in this present study are the first reporting of Tetraspora sp. CU2551 as a potential alternate source for carotenoid pigment production.
  • Loading...
    Thumbnail Image
    Some of the metrics are blocked by your 
    consent settings
    Item type:Publication,
    Influence of exogenous growth-regulators on physiological and growth processes of dwarf mandarin cv. ‘Miagava-Vase’
    (2021-09-01)
    Belous, O.
    ;
    Soytong, K.
    ;
    Vasileyko, M.
    The influence of biologically active substances of a new generation as growth regulators on the process of ovary fall, improving the quality of fruits and increasing the adaptive potential of tangerine was investigated. The tested citrus plants are dwarf mandarin cv. ‘Miagava-Vase’. The tested plants have grown since in 1986 at Plantation of Subtropical Scientific Centre, Sochi, Russia. The experimental scheme clarified the three options as exogenous growth-regulators: obstaktin (5 ml/l water); nanoelicitor (1 ml/l water) and siliplant (5 ml/l water). The content of chlorophyll and carotenoids are determined; the assessment of the functional state of plants was carried out on the slow induction of chlorophyll fluorescence. It was found that the treatment of plants with growth regulators did not affect the content of green pigments in the leaves. In the processing microbial nanoelicitor and siliplant increased the number of carotenoids, thereby enhancing the defense reactions. Treatment with growth regulators led to an improvement in the functional state of plants, which was expressed in a higher value of the viability index, especially on variants with the introduction of nanoelicitor and siliplant. The treated citrus plants with siliplant resulted to the highest number of carotenoids increased in the leaves and followed by nanoelicitor. They imply as the activation of protective reactions to the plants. The slow chlorophyll fluorescence inductiuon expressed the higher viability index in siliplant than nanoelicitor and obstaktin. The photosynthetic activity index revealed that nanoelicitor and siliplant gave the highest activity and followed by obstaktin. The coefficient of photosynthetic activity expressed that nanoelicitor, siliplant and obstaktin were significantly higher than the control. Nanoelicitor showed significantly highest plant growth and followed by siliplant and obstaktin. Siliplant treatment confirmed significantly highest mass of fruits and followed by nanoelicitor and obstaktin. The fruits diameter was higher in siliplant than obstaktin and nanoelicitor. The treated citrus leaves with nanoelicitor encountered scoparone, a phytoalexin associated with resistance of Citrus to root rot.