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Biosurfactant from Streptomyces zaomyceticus HUS20 isolated from the Indian Himalayan Region (IHR) and its antibacterial activity
(2025-07-01)
Kishorekumar, A.
;
Radhakrishnan, M.
;
Manigundan, K.
;
Ranjani, S.
;
Thenmozhli, G. S.
Actinobacteria, which are known to synthesize bioactive molecules with significant industrial and medicinal applications. In order to assess the possibility for producing biosurfactant (BS), this study identified culturable actinobacteria from the high-altitude soils of Drama Valley, Dugtu Village in the Indian Himalayan Region (IHR). Actinobacteria were isolated from pre-treated soil samples using nutrient agar, starch casein nitrate agar, and actinomycetes isolation agar, which were enhanced with 1% crude oil. Using the haemolytic assay, oil displacement test, emulsification activity, drop collapse assay, and penetration assay, morphologically different colonies were proved for the production of BS. In this study, a promising biosurfactant-producing strain, strain HUS20 showed considerable emulsification index (65%) and a 30 mm zone in the oil displacement test. Using 16S rRNA sequencing, the strain HUS20 was shown to be 100% identical to Streptomyces zaomyceticus (GenBank accession: OQ996835.1). in HR LC MS analysis, the crude biosurfactant extract showed the presence of more than 164 metabolites. Additionally, the crude biosurfactant demonstrated strong antibacterial action against foodborne pathogens, with Bacillus cereus showing the greatest suppression (24 mm zone at 100 mg/ml). The potential of actinobacteria from harsh Himalayan habitats as a useful resource for the synthesis of biosurfactants is highlighted by this work. Streptomyces zaomyceticus HUS20 is found to be promising strain for isolation of potential biosurfactant molecule.
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Isolation and screening of fish gut actinomycetes for antibacterial activity against Uropathogenic Escherichia coli
(2023-09-01)
Jisha, K.
;
Gayathri, G.
;
Gopikrishnan, V.
;
Vareeket, R.
;
Prabha, T. R.
Actinobacteria are among the most studied prokaryotes due to their propensity to create microbial bioactive compounds. Actinobacterial members can be found in a wide variety of aquatic and terrestrial settings, incorporating marine habitats. Actinomycetes isolated from the marine environment are gaining popularity due to their diverse structures and unique biological properties of the secondary metabolic products. The research was investigated the antibacterial properties of actinobacteria associated with gut of marine fish against human pathogen. Approximately 20 morphologically distinct strains obtained from Engraulis sp. (anchovy fish) and Trachurus trachurus (horse mackerel) were proved for antibacterial efficacy against Extended spectrum beta-lactamase (ESBL) producing uropathogenic Escherichia coli (UPEC). The actinobacterial strain HM7 expressed antibacterial effect against UPEC.
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Exploration of earthwormcast associated actinobacteria for plant growth promoting properties
(2023-01-01)
Ashok Kumar, S.
;
Karthiga, P.
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Rajalakshmi, G.
;
Gopikrishnan, V.
;
Radhakrishnan, M.
Due to their wide varieties of chemicals with a variety of biological actions, earthworms, cast, and gut are potential sources of significant biotechnological interest. The finding new microorganisms in peculiar and uncharted habitats were investigated. Actinobacteria are exceptional makers of secondary metabolites and have a wide range of bioactive substances of economic significance. In the current investigation, actinobacteria from earthworm casting were isolated for their potential antibacterial capabilities. Earthworm cast from the Kanchipuram agricultural area had a total of 12 actinobacterial colonies. Human and plant pathogens such as Staphylococcus aureus, Enterococcus faecalis, Escherichia coli, Klebsiella pneumonia, Pseudomonas aeruginosa and Ralstonia solanacearum were tested against each actinobacterial cultures. The selected potential culture was examined for the presence of Indole Acetic Acid (IAA), Phosphate (P) solubilization, extracellular enzymes such as cellulase and amylase. For the promising active trains, optimization, characterization and taxonomy were carried out. Colonies with various morphologies were separated and subcultured for testing their activities. Six of the twelve isolates proved positive for antibacterial activity against one or more pathogens. Of the six isolates tested, strain EWCA 21 was chosen for further screening because it had the greatest effectiveness against tested pathogens. IAA, Phosphate (P) solubilization, and extracellular enzyme activities such amylase and cellulase were demonstrated in EWCA21. Carbon and nitrogen sources expressed an increase in activity and output during optimization. The putative strain was determined to be Streptomyces based on phenotypic analysis. The study concluded that the strain EWCA21 would be developed as a crucial resource for biofertilizer applications.
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A novel of bioprospecting of earthworm cast associated actinobacteria for its antimicrobial properties-Short communication
(2021-03-01)
Gopikrishnan, V.
;
Radhakrishnan, M.
;
Joseph, J.
;
Abirami, B.
;
Manigundan, K.
Among bacteria, the actinobacteria are the most significant source of bioactive compounds and many industrially important by products in use today. The present study designed to isolate the actinobacteria from earthworm cast for its antimicrobial properties. Totally 56 actinobacterial colonies were isolated from earthworm cast of Kanchipuram agricultural area. Of which, 36 morphologically different colonies were isolated and identified which mostly belong to Streptomyces. In antimicrobial activity, among 36 isolates, 12 were showed antimicrobial activity against one or more tested human pathogens eg Staphylococcus aureus, Enterococcus faecalis, Escherichia coli, Klebsiella pneumoniae and Pseudomonas aeruginosa. So, the study revealed earthworm cast associated actinobacteria can be recorded as an imperative resource for bio-prospecting that could produce novel bioactive metabolites.
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Micromonospora soli sp. nov., isolated from rice rhizosphere soil
(2016-03-01)
Thawai, Chitti
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Kittiwongwattana, Chokchai
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Thanaboripat, Dusanee
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Laosinwattana, Chamroon
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Koohakan, Prommart
An actinomycete strain SL3-70T was isolated from a rice field and characterised using a polyphasic approach. The morphological and chemotaxonomical characteristics of strain SL3-70T indicate that it belongs to the genus Micromonospora. The phylogenetic analysis of the nearly complete 16S rRNA gene sequence revealed that strain SL3-70T is a member of the genus Micromonospora, and is closely related to Micromonospora echinaurantica DSM 43904T (99.1 % 16S rRNA gene sequence similarity) and Micromonospora kangleipakensis MBRL 34T (98.8 %). DNA–DNA relatedness between strain SL3-70T and its relatives ranged from 21.2 % ± 0.6 to 38.7 % ± 0.4. The results obtained from our study indicate that strain SL3-70T represents a novel species of the genus Micromonospora, for which the name Micromonospora soli sp. nov. is proposed. The type strain is SL3-70T (=BCC 67268T; =NBRC 110009T).

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