Kittiwongwattana, Chokchai

Preferred name

Alternative Name

Kittiwongwattana, Chockchai

Main Affiliation

School of Science

chokchai.ki@kmitl.ac.th

Now showing 1 - 10 of 26

Some of the metrics are blocked by your
consent settings
Micromonospora endophytica sp. nov., an endophytic actinobacteria of Thai upland rice (Oryza sativa)
(2015-11-01)
Thanaboripat, Dusanee
;
Thawai, Chitti
;
Kittiwongwattana, Chokchai
;
Laosinwattana, Chamroon
;
Koohakan, Prommart
An actinobacterial strain, DCWR9-8-2 T, was isolated from a leaf of Thai upland rice (Oryza sativa) collected in Chumporn province, Thailand. Strain DCWR9-8-2 T is Gram-stain-positive aerobic bacteria that produce single spores directly on the vegetative hypha. Cell wall peptidoglycan of this strain exhibits meso-diaminopimelic acid and glycine, the reducing sugars of whole-cell hydrolysate are arabinose, glucose, ribose, xylose and small amount of mannose. The phospholipid profiles in the membrane are comprised of phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannosides. The major menaquinones are MK-9(H 4) and MK-10(H 6). The diagnostic cellular fatty acids are iso-C 16:0 and iso-C 15:0. The G+C content of the genomic DNA is 72.5 mol%. The result of 16S rRNA sequence analysis of the strain revealed that this strain was closely related to Micromonospora auratinigra TT1-11 T (99.25%). On the other hand, the result of gyrB gene sequence analysis revealed that this strain was closed to M. eburnea JCM 12345 T (96.30%). In addition, a combination of DNA-DNA hybridization results and some phenotypic properties supported that this strain should be judged as a novel species of the genus Micromonospora, for which the name M. endophytica sp. nov. is proposed. The type strain is DCWR9-8-2 T (=BCC 67267 T =NBRC 110008 T).
Some of the metrics are blocked by your
consent settings
Gel purification of gDNA for next-generation sequencing applications
(2022-07-01)
Utthiya, Supanut
;
Wonnapinij, Passorn
;
Napaumpaiporn, Pondpan
;
Kittiwongwattana, Chokchai
;
Sakulkoo, Jenjira
We demonstrate that gDNA can be conveniently and efficiently isolated and purified using standard agarose gel electrophoresis, band excision and gel purification. This method yields a substantial amount at microgram levels of gDNA per gel cleanup with high purity. An RNase A treatment step can be omitted. The quality of gDNA is suitable for next-generation sequencing, resulting in >10 Mb reads and high-quality read data (Phred score >28 up to 100 of 150 base reads). Furthermore, the gDNA can be kept intact in a gel slice for several days. This method has been tested for dictyostelids, bacteria and plants.
Some of the metrics are blocked by your
consent settings
Modified natural seawater as growth medium for the halotolerant cyanobacterium Aphanothece halophytica to increase lipid content for biodiesel production
(2025-02-01)
Thongtha, Sitthichai
;
Aryusuk, Kornkanok
;
Kittiwongwattana, Chokchai
;
Incharoensakdi, Aran
;
Phunpruch, Saranya
Biodiesel derived from cyanobacterial oils becomes attractive as an efficient renewable energy. The present study aims to optimize growth and lipid production of the halotolerant unicellular cyanobacterium Aphanothece halophytica cultivated in natural seawater. In this study A. halophytica was able to grow in natural seawater when supplemented with low concentration of NaNO<inf>3</inf>, whereas no growth occurred without supplementation. The specific growth rate of 0.230 day-1 and cell concentration of 25.17 x 106 cells mL-1 were achieved in A. halophytica cultivated in natural seawater supplemented with 17.6 mM NaNO<inf>3</inf> and Turk Island salt solution (suitable natural seawater; SNSW) for 14 days. This growth rate was comparable to that of cells grown in normal BG11 plus Turk Island salt solution. The lipid content and fatty acid profiles of A. halophytica varied with changes in NaCl concentrations. The highest lipid content of 50.47 % and lipid productivity of 48.33 mg L-1 day-1 were obtained in cultures supplemented with 1.89 mmol C-atom L-1 glucose and 0.75 M NaCl. The optimal medium pH and cultivation temperature for lipid production was 7.5 and 25-35 °C, respectively. When cultivating A. halophytica in optimized SNSW with various NaCl concentrations, the highest contents of linoleic and linolenic acids, and the lowest contents of palmitic, stearic, and oleic acids were observed with 0.75 M NaCl. In contrast, cultures grown in optimized SNSW with 0.5 M NaCl showed fatty acid methyl ester profiles rich in monounsaturated fatty acids, which are favorable for high-quality biodiesel production.
Some of the metrics are blocked by your
consent settings
Correlation of Antifungal Activities and Nonribosomal Peptide Synthetase Gene Expression of Bacillus siamensis 1021
(2022-03-01)
Nuwong, Warinya
;
Kittiwongwattana, Chokchai
Members of the genus Bacillus were biocontrol agents against various phytopathogenic fungi. Several factors affected the regulation and production of nonribosomal peptides (NRPs) in Bacillus species. The aim of this study was to examine how culture media influenced the antagonistic activities and gene expression of Bacillus siamensis 1021. The bacterial supernatant from potato dextrose broth (PDB) exhibited higher inhibitory effects against mycelia of Pyricularia oryzae and Colletotrichum fructicola, when compared with those from nutrient broth (NB) and minimal salt medium (MSM). However, the biocontrol activities of these supernatants were not significantly different when they were tested against P. oryzae and C. fructicola conidia. Additionally, the bacterial supernatant from PDB significantly reduced the disease severity caused by P. oryzae in rice seedlings when compared with the water-treated control group. The genome of strain 1021 was sequenced, and biosynthetic gene clusters of nonribosomal peptides surfactin, fengycin and bacillibactin were identified. Effects of PDB, NB and MSM media on expression levels of core biosynthetic genes of surfactin (srfAA), fengycin (fenC) and bacillibactin (dhbE) gene clusters were determined by reverse-transcription quantitative PCR. fenC expression was significantly increased in PDB and corresponded with the antagonistic activities against fungal mycelia. Conversely, expression of the regulatory genes comA and codY in PDB were highly reduced in PDB, indicating their negative relation with fenC expression. This expression analysis indirectly suggested that fengycin was potentially the bioactive compound of B. siamensis 1021 against P. oryzae and C. fructicola.
Some of the metrics are blocked by your
consent settings
Effects of sequence and expression of eight anthocyanin biosynthesis genes on floral coloration in four dendrobium hybrids
(2015-01-01)
Kriangphan, Nattaphan
;
Vuttipongchaikij, Supachai
;
Kittiwongwattana, Chokchai
;
Suttangkakul, Anongpat
;
Pinmanee, Phitsanu
Understanding the control of anthocyanin biosynthesis is beneficial to genetic improvement for floral production in Dendrobium orchids. Full-length cDNA of CHS, CHI1, CHI2, F3H, DFR, ANS, F3'5'H, and FLS was isolated from Dendrobium hybrids with purple, peach, white and greenish white flowers. Analysis of the deduced amino acid sequences and gene expression levels of the eight genes suggested potential causes of color variation among the hybrids. Peach hybrid (SC) was likely due to changes in anthocyanin production from cyanidin to pelargonidin through mutations in F3'H, and the low color intensity was likely derived from the low expression levels of CHI1 and CHI2. In addition, white hybrid (RW) was likely caused by several mutations in F3H and/or high expression levels of FLS, an enzyme that converts color flavonoid intermediates into colorless flavonols. Simultaneous loss of F3H, DFR, and ANS expression observed in another white hybrid (JW) indicated that an alteration of anthocyanin regulatory controls was likely the cause of white coloration. Furthermore, analysis of hybrid mutants bearing pale and dark flowers demonstrated the influence of the expression of anthocyanin genes on the intensity of flower colors. Data obtained from this work could contribute to new strategies for future orchid breeding.
Some of the metrics are blocked by your
consent settings
Rhizobium paknamense sp. nov., isolated from lesser duckweeds (Lemna aequinoctialis)
(2013-10-01)
Kittiwongwattana, Chokchai
;
Thawai, Chitti
A Gram-stain-negative, rod-shaped bacterium was isolated and designated strain L6-8T during a study of endophytic bacterial communities in lesser duckweed (Lemna aequinoctialis). Cells of strain L6-8T were motile with peritrichous flagella. The analysis of the nearly complete 16S rRNA gene sequence indicated that strain L6-8T was phylogenetically related to species of the genus Rhizobium. Its closest relatives were Rhizobium borbori DN316T (97.6 %), Rhizobium oryzae Alt 505T (97.3 %) and Rhizobium pseudoryzae J3-A127T (97.0 %). The sequence similarity analysis of housekeeping genes recA, glnII, atpD and gyrB showed low levels of sequence similarity (,91.5 %) between strain L6-8T and other species of the genus Rhizobium with validly published names. The pH range for growth was 4.0-9.0 (optimum 6.0-7.0), and the temperature range for growth was 20-45 °C (optimum 30 °C). Strain L6-8T tolerated NaCl up to 2% (w/v) (optimum 1% NaCl). The predominant components of cellular fatty acids were C<inf>19: 0</inf> cyclo ω8c (31.32 %), summed feature 8 (C<inf>18: 1</inf>ω7c and/or C<inf>18: 1</inf>ω6c; 25.39 %) and C<inf>16: 0</inf> (12.03 %). The DNA G+C content of strain L6-8T was 60.4 mol% (T<inf>m</inf>). nodC and nifH were not amplified in strain L6-8T. DNA-DNA relatedness between strain L6-8T and R. borbori DN316T, R. oryzae Alt505T and R. pseudoryzae J3-A127T was between 11.2 and 18.3 %. Based on the sequence similarity analyses, phenotypic, biochemical and physiological characteristics and DNA-DNA hybridization, strain L6-8T could be readily distinguished from its closest relatives and represents a novel species of the genus Rhizobium, for which the name Rhizobium paknamense sp. nov. is proposed. The type strain is L6-8T (=NBRC 109338T=BCC 55142T). © 2013 IUMS.
Some of the metrics are blocked by your
consent settings
Medium Effect on Antagonistic Activity and Detection of Nonribosomal Peptide Synthetase Genes in Epiphytic Bacillus Strains
(2021-01-01)
Apimeteethamrong, Suchitra
;
Kittiwongwattana, Chokchai
The biosynthesis of non-ribosomal peptides (NRPs) in biocontrol bacteria was one of the major antagonistic mechanisms for their application in agriculture. Bacillus spp. 1021, 2211 and 3210 were previously shown to inhibit mycelial growth of the leaf blast fungus Pyricularia oryzae. Here, we aimed to further study the antagonistic mechanism in those three strains. Cell-free supernatants obtained from bacteria grown in potato dextrose broth (PDB) exhibited a higher degree of inhibition against P. oryzae when compared to those obtained from nutrient broth (NB). This indicated the effect of culture media in the production of extracellular antibiotic compounds by these strains. Phylogenetic analysis of their partial 16S rRNA gene sequences indicated a close relationship between the three strains and Bacillus siamensis KCTC13613T, Bacillus amyloliquefaciens DSM7T and Bacillus velezensis CR-502T. Complete genome sequences of these Bacillus species were analyzed on the antiSMASH server to identify the presence of NRP biosynthesis gene clusters. Non-degenerate primers were designed for the detection of the core biosynthesis genes for surfactin (srfAA), fengycin (fenC) and bacillibactin (dhbF). All three genes were amplified in strains 1021 and 2211, while only srfAA and dhbF were detected in strain 3210. Phylogenetic analysis of the deduced amino acid sequences indicated that the sequences of strain 1021 were distinct from those of strains 2211 and 3210. This result indirectly suggests the possibility of NRP production as the antagonistic mechanism of these three strains.
Some of the metrics are blocked by your
consent settings
Genetic relationship of Coccinia grandis (L.) Voigt accessions, based on RAPD and ISSR markers
(2022-01-01)
Kittiwongwattana, Chokchai
Coccinia grandis (L.) Voigt or ivy gourd is widely distributed in nature and has potential for medicinal uses. However, there is little information on the genetic diversity of wild populations in Thailand. Here, 15 ivy gourd accessions were collected from Bangkok and nearby provinces. Angled and lobed leaves were observed. DNA fingerprints were generated from twelve random amplified polymorphic DNA (RAPD) and twelve inter-simple sequence repeat (ISSR) primers. The level of polymorphism in the RAPD profiles was higher than that of the ISSR profiles. Dice's similarity coefficient from RAPD data ranged from 0.73 to 0.82 and was lower than that from the ISSR data (0.88-1.00). The unweighted pair group method with arithmetic mean dendrogram, based on combined RAPD and ISSR data, was similar to the RAPD dendrogram but distinct from the ISSR dendrogram. Two major clusters were found with eleven and four accessions. The genetic relationship between some accessions was relatively consistent with either their locations or leaf shapes. The result indicated that the RAPD and ISSR techniques were suitable for the genetic study of ivy gourd. It also provided the basis for future studies with a higher number of samples for a better understanding of ivy gourd genetic diversity in Thailand.
Some of the metrics are blocked by your
consent settings
Micromonospora oryzae sp. Nov., isolated from roots of upland rice
(2015-11-01)
Kittiwongwattana, Chokchai
;
Thanaboripat, Dusanee
;
Laosinwattana, Chamroon
;
Koohakan, Prommart
;
Parinthawong, Nonglak
An actinomycete strain, designated CP2R9-1T, was isolated from root internal tissues of upland rice (Oryza sativa). Based on a polyphasic approach, strain CP2R9-1T was characterized as a member of the genus Micromonospora. meso- Diaminopimelic acid and 3-OH-diaminopimelic acid were present in the cell-wall peptidoglycan. The polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannosides, two unidentified phospholipids and four unidentified polar lipids. Predominant menaquinones were MK-9(H<inf>4</inf>), MK-9(H<inf>6</inf>) and MK-10(H<inf>4</inf>). Whole-cell sugars consisted of ribose, xylose, arabinose and glucose. Phylogenetic analysis of the nearly complete 16S rRNA gene sequence suggested that strain CP2R9-1T was closely related to Micromonospora haikouensis 232617T (99.32% similarity), Micromonospora carbonacea DSM 43168T (99.18%) and Micromonospora krabiensis MA-2T (99.16%). Strain CP2R9-1T was distinct from its closest relatives based on low levels of DNA–DNA relatedness (21.3¡0.1– 41.7¡0.7%) and phenotypic differences. The results presented in this study showed that strain CP2R9-1T represents a novel species of the genus Micromonospora, for which the name Micromonospora oryzae sp. nov. is proposed. The type strain is CP2R9-1T (5BCC 67266T5NBRC 110007T).
Some of the metrics are blocked by your
consent settings
Diversity and plant growth promoting activities of rice epiphytic bacteria
(2019-01-01)
Aphimeteetamrong, Suchitra
;
Kittiwongwattana, Chokchai
Plant-associated bacteria have been known for their direct and indirect abilities to promote plant growth. Because of their benefits on plants, they are generally recognized as plant growth promoting bacteria (PGPB). In the present study, epiphytic bacteria were isolated from surfaces of roots, stems and leaves of rice plants that were grown in organic farms. Of 113 bacterial isolates, 68 and 45 isolates were stained Gram-positive and Gram-negative, respectively. Based on their cell shapes, 106 isolates were bacilli while seven isolates were cocci. The use of the 16S rRNA gene sequence analysis indicated that they were members of phyla Fimicutes (54.87%), Proteobacteria (38.05%) and Bacteroidetes ( 7. 08% ). All isolates were assessed for their abilities in nitrogen fixation, phosphate solubilization, siderophore production, auxin production and 1-aminocyclopropane-1-caboxylic acid (ACC) deaminase activity. The result showed that 91 isolates were tested positives for at least one activity. The antagonistic activity against the pathogenic fungus Pyricularia oryzae was also tested. Twenty-three isolates inhibited the growth of the fungal mycelia. The percentage of growth inhibition of these isolates against P. oryzae ranged from 73.10+3.24% to 100.00+0.00%. The protease and chitinase activities were found in 19 and 2 isolates, respectively. However, crude preparation of bioactive compounds from bacterial cultures yielded negative results. Based on the results obtained in this study, the surfaces of rice plants were colonized by various bacteria with potentials in plant growth promotion.

Kittiwongwattana, Chokchai

Filters

Settings

Sort By

Results per page