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    A scanner-based microplate platform for high-throughput oxytetracycline detection using diazotization-coupling chemistry with greenness evaluation
    (2026-06-01)
    Puttasa, Papawarin
    ;
    Ngernpimai, Sawinee
    ;
    Tippayawat, Patcharaporn
    ;
    Park, Myoung‑Hwan
    ;
    Teerasong, Saowapak
    Routine oxytetracycline (OTC) monitoring of aquaculture effluents, water sources, and pharmaceutical products is essential for environmental management and regulatory compliance. However, existing analytical methods remain largely instrumental and low-throughput. Herein, a microvolume, high-throughput, scanner-based colorimetric microplate platform is developed for OTC determination using a sensitive and selective diazotization-coupling chemistry with RGB digital image analysis. Sulfanilamide is diazotized with nitrite to generate reactive diazonium species, which subsequently couple with OTC to form a strongly colored yellow-orange azo dye. Only microliter-scale volumes of reagents and samples are required in each well, and the reaction is completed within a few minutes, enabling simultaneous analysis of up to 96 samples. The microplate is imaged with a flatbed scanner, and color responses are quantified using ImageJ software. A linear calibration range of 1 − 40 μM was obtained with a detection limit of 0.33 μM. The platform was successfully applied to OTC determination in aquaculture effluents, tap water, drinking water, and pharmaceutical samples. It exhibited high selectivity against other antibiotics with no observable cross-reactivity. The green analytical procedure index (GAPI) confirmed the method’s environmental friendliness. Overall, the scanner-based microplate platform offers a simple, low-cost, portable, and high-throughput alternative to conventional OTC assays.
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    Investigating antagonistic activity of fish gut bacterial isolates against aquaculture pathogens: Short Communication
    (2024-05-01)
    Prakash, M. P.K.
    ;
    Padmanaban, D.
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    Loganthan, C.
    ;
    Palaniswamy, K.
    ;
    Krishnamoorthy, K.
    Aquaculture worldwide suffers significant economic losses and high fish mortality due to bacterial diseases. Antibiotics have been used extensively to combat these infections owning to the overuse of antibiotics, Drug-resistant bacteria can emerge. Studies on probiotics, which provide improved growth and disease resistance, are becoming more and more popular as an antibiotic substitute, Bacteria derived from a marine setting such as fish gut might be putative candidate for aquaculture probiont. In vitro antagonistic activity against fish pathogens has been used to assess the antimicrobial properties of bacteria isolated from the gut of Sardinella longiceps and Rastrelliger Kanagurta.
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    Amla extract's impact on pre-weaning piglet diarrhea: An investigation into the therapeutic potential of Indian Gooseberry (Phyllanthus emblica L.)
    (2024-03-01)
    Thammakarn, C.
    ;
    Kaewhom, P.
    ;
    Klompanya, A.
    ;
    Srikijkasemwat, K.
    The crude extract of Amla was found to be effective in treating pre-weaning piglet diarrhea. In this study, the group given a 25% extract concentration showed the most rapid recovery (4.80±0.57 days) and highest body weight gain (3240.00±45.83 g/piglet) over 15 days, outperforming the control group. Additionally, treatment costs were significantly lower in the 25% Amla crude extract group (0.039 USD per piglet) compared to the control group (0.052 USD per piglet). These findings suggested that Amla crude extract could be a cost-efficient alternative for managing pre-weaning piglet diarrhea, especially at a 25% concentration. Moreover, it addressed the issues of treatment expenses and antimicrobial resistance.
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