Srikijkasemwat, Kanokrat

Preferred name

Alternative Name

Srikijkasemwat, K.

Main Affiliation

School of Agricultural Technology

kanokrat.sr@kmitl.ac.th

Now showing 1 - 10 of 21

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Thymol-carvacrol supplementation in broilers: impact on performance, blood biomarkers, and gut health
(2025-03-01)
Tiyaprasertkul, P.
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Phungkeha, P.
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Srikijkasemwat, K.
;
Philatha, A.
;
Rassmidatta, K.
The stress conditions are induced by a high-dose live coccidiosis vaccine, and thymol-carvacrol supplementation in broilers were significantly improved the feed conversion ratio (FCR) during the starter and grower phases as compared to the negative control. In contrast, antibiotic supplementation resulted in better growth performance throughout the rearing period. In addition, antibiotic supplementation lowered serum levels of the oxidative stress marker malondialdehyde (MDA) and the pro-inflammatory cytokine interleukin-17 (IL-17), with thymol-carvacrol supplementation showing comparable but less pronounced effects, followed by the negative control. In addition, antibiotic supplementation improved intestinal morphology as evidenced by the decreased crypt depth and increased villus height to crypt depth ratio, followed by thymol-carvacrol supplementation compared to the non-supplemented groups. These results suggest that thymol-carvacrol supplementation can improve the gut health and performance of broilers under stress conditions.
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Efficacy of two primer sets used in the sex identification of rufous-winged buzzard (Butastur liventer)
(2021-01-01)
Kaewhom, Paitoon
;
Srikijkasemwat, Kanokrat
Background: The rufous-winged buzzard (Butastur liventer) belongs to the order Accipitriformes, which is monomorphic, resulting in the difficulty to identify the gender. However, sex determination is important for predicting the sex ratio of this buzzard in nature in order to avoid its extinction. Aim: We aimed to develop a primer set that is able to sex the rufous-winged buzzard through polymerase chain reaction (PCR) amplification and compare the efficacy of the two sets of primers by using PCR technique. Methods: In the following, sensitivity refers to the smallest DNA concentration that allowed us to accurately sex a bird and specificity refers to the ability to clearly distinguish the sex based on the visual appearance of the bands. Blood samples were collected from captive buzzards. The DNA was extracted from them and was diluted to 50, 25, 10, 5, 2.5, 1.67, and 1 ng/µl. Two sets of primers, including P2/NP/MP and 2550F/2718R, were used to amplify the chromo-helicase DNA binding (CHD) gene of known gender buzzards using the PCR process to determine gender and to compare their sensitivity. To measure specificity, both primers were used to amplify CHD gene fragment of other unknown gender birds. Results: The lowest concentration of the DNA template where P2/NP/MP could amplify DNA fragments was 1 ng/µl, and this set of primers could identify the gender of all birds correctly, giving 100% specificity. On the other hand, the 2550F/2718R could amplify the DNA fragments from 5 ng/µl, and it had only 78% specificity. Conclusion: The P2/NP/MP primer set was able to correctly identify the gender of rufous-winged buzzard through PCR amplification with high specificity and sensitivity.
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A molecular survey of Theileria spp. in Ruminants in the Thailand-Cambodia border region
(2022-01-01)
Kaewhom, P.
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Srikijkasemwat, K.
Theileriosis is caused by Theileria spp. Parasites which transmitted by various species of ticks including Amblyomma, Haemaphysalis, Hyalomma and Rhipicephalus. This pathogen can be found in red blood cells and white blood cells of farmed animals, including cattle, buffaloes, goats and sheep. Theileria spp. leads to a high morbidity in small ruminants, economic loss in agricultural production and time spent on infection prevention. It is necessary to detect the infection in a farm, as there are generally no clearly recognisable clinical signs. Our results revealed that infected animals displayed 230 bp DNA fragments, which is the length of the V4 region of the 18S rRNA gene of Theileria spp. The overall prevalence of Theileria spp. in ruminants farmed in Sa Kaeo Province, the border between Thailand and Cambodia, was 9% (29/314). The prevalence of Theileria spp. in meat cattle (6%), buffaloes(6%), meat goats (6%) and crossbred meat sheep (6%). It was also found that the prevalence of Theileria spp. was linked to neither species nor gender (p>0.05). Polymerase Chain Reaction was used to analyse Theileria spp. since it is very sensitive, specific and fast. Our analysis can be used to prevent and control the spread of Theileria infection in ruminants. Furthermore, this information may guide implemention of a policy on animal movement within the border regions.
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Effect of feeding sunflower meal on the performance and carcass characteristics of broiler chickens
(2017-01-01)
Sangsoponjit, Settasit
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Suphalucksana, Wichai
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Srikijkasemwat, Kanokrat
The objective of the experiment was to evaluate the effect of feeding sunflower meal on the performance and carcass characteristics of broiler chickens. A total of six hundred day-old male Arbor Acre chicks were allotted to a completely randomized experimental design. There were four levels of 0%, 4%, 8%, and 12% sunflower meal with five replicates each for forty-two days trial. The inclusion of different concentrations of sunflower meal did not alter the productive performance of the birds. There were not significant differences in body weight, feed intake, feed conversion ratio, mortality, and the European Production Efficiency Factor (E.P.E.F.) index among the experimental groups (P≥0.05). During 22-42 days of trial, the inclusion of 12 % sunflower meal was the highest in feed intake but it was only significant differences between the inclusion of 8 % sunflower meal group (p<0.05). However, the inclusion of 12 % sunflower meal had higher feed intake and mortality than the other groups for the whole period of trial (p>0.05). The E.P.E.F. index were 299.76, 320.25, 343.22, and 298.90 for the birds fed with 0 %, 4 %, 8 %, and 12 %, of sunflower meal in the diet, respectively. The inclusion of sunflower meal from 4 %, 8 %, and 12 % had no effect on carcass percentage and cut yield of broilers. They were breast, fillet, three joint wings, thigh, and drumstick, including abdominal fat
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Using leucaena to improve the quality of pineapple plant silage
(2017-01-01)
Suphalucksana, Wichai
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Sangsoponjit, Settasit
;
Srikijkasemwat, Kanokrat
The objective of this study was to improve the quality of pineapple plant silage by leucaena supplementation and using it as roughage during the shortage of green forages. There were 5 treatments as follows :- 1. Pineapple plant 100%, 2. pineapple plants mixed with leucaena 5%, 3. pineapple plants mixed with leucaena 10%, 4. Pineapple plants mixed with leucaena 15% and 5. grass 100%. The Completely Randomized Design (CRD) with four replications each was used in this experiment. All treatments were put in 1%NaCL<inf>2</inf> and tightly sealed in plastic containers. They were stored at room temperature for 21 days. The silage treatments were evaluated on physical characteristics, chemical composition and fibre analysis. The physical characteristic showed that the color of treatment 1 was a yellow green color, a good characteristic of silage. For treatments 2, 3 and 4 there was a light brown color. Treatment 5 was a dark brown in color. The aromas of all silage treatments were aromatic and acidic like pickled fruit. The evaluation of chemical composition showed a highly significant difference among the treatment groups (p < 0.01). Treatment 4 was the highest in protein and metabolizable energy percentage with the averages of 8.19% and 3,697.17 kcal/k, respectively. The percentage of crude fibre was reduced with the increase of leucaena in the silage. Treatment 5 was the highest in crude fibre. The pineapple plant silage mixed with leucaena could improve the quality of silage for ruminants.
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Fermented juice of epiphytic lactic acid bacteria and molasses addition on the fermentation characteristics and nutrient compositions of sorghum silage
(2019-08-01)
Lukkananukool, Achara
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Srikijkasemwat, Kanokrat
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Promnaret, Amphai
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Aung, Min
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Kyawt, Yin Yin
This study was conducted to determine the effect of fermented juice of epiphytic lactic acid bacteria (FJLB) and molasses addition on the fermentation characteristics and nutrient compositions of sorghum silage. Sorghums were chopped into pieces of 1-2 cm length and molasses 5% (w/w) and FJLB 1% (v/w) of fresh material were added as silage additives. No significant effects on physical characteristics except pH, lowest in molasses silage, were noted. The higher organic matter and lower fibre contents were observed in molasses silage. Total carbohydrate, non fibre carbohydrate, total digestible nutrients, digestible dry matter and dry matter intake were highest in molasses silage. The higher acetic, propionic and lactic acid concentrations were observed in FJLB silage. The lactic acid bacteria (LAB) count was highest in FJLB silage, and lowest in control silage. The low volatile based nitrogen (VBN) and high V-score, indicators of good quality silages, were generally observed in molasses and FJLB rather than control silages. This experiment indicated that FJLB can be alternatively used as additive instead of using molasses in sorghum silage; however more inclusion level of molasses and FJLB should be used to ensure good quality silage.
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Effects of thymol–carvacrol combined with organic acids on carcass traits, meat quality, and stress indicators in broilers under enteric disease challenge
(2026-01-01)
Busayakanit, P.
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Srikijkasemwat, K.
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Philatha, A.
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Rassmidatta, K.
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Ruangpanit, Y.
The effects of a feeding program combining thymol–carvacrol and organic acids were evaluated as an alternative to an antibiotic growth promoter (AGP) in broilers under a coccidial challenge. A total of 750 male Ross 308 chicks were assigned to a negative control (T1), an AGP group with bacitracin methylene disalicylate (T2), or a thymol-carvacrol–organic acid group (T3). Slaughter weight, dressing percentage, retail cut yields, and meat quality traits did not differ among treatments (P > 0.05). T2 had the highest number of birds without foot pad lesions, significantly exceeding T1 and comparable to T3, which also outperformed T1 (P < 0.05). T2 recorded the fewest mild and severe lesions, while T3 showed a numerical reduction in severe lesions compared with T1. Both T2 and T3 reduced lesion severity relative to T1, with T2 showing the most favorable scores and T3 demonstrating meaningful potential as a non-antibiotic alternative. Thigh meat malondialdehyde (MDA) levels increased during storage (P < 0.01), but no treatment effects were observed (P > 0.05). Serum MDA and the proportions of lymphocytes, monocytes, eosinophils, and basophils were unaffected; however, heterophil percentage and the heterophil-to-lymphocyte ratio were significantly higher in T1 than in T2 and T3 (P < 0.01), indicating reduced physiological stress with both AGP and thymol-carvacrol–organic acid supplementation. Overall, thymol–carvacrol with organic acids-maintained carcass yield and meat quality while mitigating stress responses and improving foot pad health, supporting its potential as a viable alternative to AGPs in broilers under enteric challenge.
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The effect of adding mung bean meal supplementation on Napier Pakchong 1 silage on fermentation quality and nutrient composition
(2018-12-01)
Boonkoed, S.
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Suphalucksana, W.
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Sitthigripong, R.
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Srikijkasemwat, K.
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Mitchaothai, J.
The effect of mung bean meal supplementation on Napier Pakchong 1 silage on fermentation quality and nutritional value was investigated. The results found that pH of all treatments of mung bean meals on Napier Pakchong 1 silage ranges from 4.17-4.73 was the lowest value found in the treatment 1 whereas highest value found in the treatment 6 (P<0.01). When volatile basic nitrogen (VBN) in silage was recorded, the VBN in the ailage of the treatment 5 had the highest value (35.56%) and followed by that of the treatment 6 (32.98%) while the lowest VBN found in the silage of the treatment 1 (8.57%) (P<0.01). Moreover, the silage in the treatment 6 was the highest nutritional proportion by increasing dry matter (DM), crude protein (CP), ether extract (EE) and gross energy (GE), while decreasing ash, neutral detergent fiber (NDF) and acid detergent fiber (ADF) (P<0.01). Thus, making silage by adding mung bean meals on Napier Pakchong 1 could improve nutritional value of silage in term of increasing DM, CP, EE and GE contents, but decreasing contents of ash, NDF and ADF. In addition, making silage from Napier Pakchong 1 grass by replacing mung bean meal at 10-20% could be applicable for preserving ruminant roughage.
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Chemical composition and diversity of lactic acid bacteria in Guinea grass based silages
(2019-05-01)
Kongsan, S.
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Srikijkasemwat, K.
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Jirajaroenrat, K.
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Suphalucksana, W.
Ensilage is considered that widespread among conserving method of fodder crops. The successes of this process depend on bacteria in lactic acid bacteria (LAB) group as existing in forage crops. The chemical composition and diversity of lactic acid bacteria in silages based on guinea grass was investigated. Result showed that the silages contained 62.55 to 76.47% moisture, 23.53 to 37.45% dry matter (DM) and 6.11 to 15.74% ash. Groundnut silage (T3) showed the lowest crude fiber (CF), neutral detergent fiber (NDF) and acid detergent fiber (ADF) contents. Two hundred and eighty-two isolates of LAB were isolated from five silage formula based on guinea grass supplementation with either lead tree leaves or groundnut. Most of isolates were gram-positive and catalase-negative bacteria. Based on morphological characteristics and PCR-RAPD analysis, the isolates were divided into 21 groups (Guinea Grass Mixed Legume Silage (GMLS) 1 to GMLS21). The phylogenetic relation of the representative strains was analyzed from the data of 16S ribosomal DNA sequencing. Prevalent of LAB found in the silages included Lactobacillus plantarum, Pediococcus acidilactici, Pediococcus pentosaceus, Enterococcus hirae, and Weissella paramesenteroides. L. plantarum was a dominant population and found distribution in all silage formula. This study revealed that the silage samples, including guinea grass, lead tree and groundnut contain mainly of L. plantarum. The information obtained can be used for the anterior design of reasonable inoculants for improvement of silage quality for cattle feed.
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Molecular identification of Anaplasma ovis and Anaplasma marginale in Sheep using PCR-RFLP method
(2021-05-01)
Kaewhom, P.
;
Srikijkasemwat, K.
Anaplasma is a protozoan blood in livestock such as cattle, goats and sheep. This pathogen leads to anaplasmosis, which is tick-borne disease, production loss and high costs due to disease prevention. Therefore, it is necessary to investigate the epidemiology of Anaplasma of livestock. The results demonstrated that the semi-nested-PCR product was approximately 120 bp. The restriction endonuclease Bst1107I only recognized the sequence (GTATAC) in semi-nested PCR product of A. marginale and cut the sequence at the position 68. However, the restriction endonuclease was not able to cut the semi-nested PCR product of A. ovis at the same position because of the different sequence (GTACGC). The prevalence of A. ovis and A. marginale infection in sheep at Sa Kaeo Province was 3.66% (3/82) and 1.22% (1/82), respectively. The overall prevalence of Anaplasma infection in sheep was 7.32% (6/82). From this result, the PCR-RFLP method could be used to isolate A. ovis and A. marginale using this technique with high specificity and safe time to identify both types of infection.
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Srikijkasemwat, Kanokrat

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