Search Results

Now showing 1 - 4 of 4

Some of the metrics are blocked by your
consent settings
Genome Insights into the Plant Growth Promoting Features of a Newly Found Microbispora sp. SCL1-1
(2024-10-02)
Dejtisakdi, Wipawee
;
Duangupama, Thitikorn
;
Thawai, Chitti
Plant growth-promoting (PGP) actinobacteria can be used to promote plant growth. Their use is a promising strategy that can be employed instead of agricultural chemical fertilizers. An actinobacterium strain, designated SCL1-1, was collected and isolated from a soil sample in a herbal garden at Pathum Thani province, Thailand. Analysis revealed that the SCL1-1 strain was a Gram-positive bacterium that formed longitudinal paired spores that were borne directly on aerial mycelia. It contained meso-diaminopimelic acid in its cell wall peptidoglycan. Moreover, madurose, which is a diagnostic sugar, was present in its whole-cell hydrolysates. 16S rRNA gene analysis revealed that the SCL1-1 strain was a member of the Microbispora and showed a close relationship to Microbispora rosea ATCC 12950T (99.6%), followed by Microbispora hainanensis DSM 45428T (99.2%). However, a genome-based polyphasic study revealed that strain SCL1-1 had a low average nucleotide identity (ANI) (<95%), and digital DNA–DNA hybridization (dDDH) value (<70%) with M. rosea ATCC 12950T and M. hainanensis DSM 45428T, indicating that strain SCL1-1 was a different species to its close relatives. Genome mining of strain SCL1-1 showed the presence of genes related to the production of indole-3-acetic acid (IAA), and siderophore, which are agents that promote plant growth. In addition, the genome of strain SCL1-1 was found in several secondary metabolite biosynthetic gene clusters, which were possibly encoded for a broad range of remarkable natural products and antibiotics.
Some of the metrics are blocked by your
consent settings
Abyssomicin derivatives from the rhizosphere soil actinomycete Microbispora rhizosphaerae sp. nov. TBRC6028
(2021-05-01)
Bunbamrung, Nantiya
;
Kittisrisopit, Suchada
;
Intaraudom, Chakapong
;
Dramae, Aibrohim
;
Thawai, Chitti
Three undescribed abyssomicin derivatives, including microbimisin, abyssomicins Z1, and Z2, were isolated from the soil actinomycete Microbispora rhizosphaerae sp. nov. TBRC6028. Chemical structures were determined by NMR spectroscopic data (1H, 13C, COSY, HSQC, HMBC, and NOESY spectra) and the absolute configurations were verified by single-crystal X-ray diffraction analyses together with the ECD spectral data. Microbimisin and abyssomicin Z1 exhibited weak antibacterial activity against Bacillus cereus with MIC values of 25.0 and 50.0 μg/mL without cytotoxicity against MCF-7 and Vero cells at the concentration of 50 μg/mL.
Some of the metrics are blocked by your
consent settings
Neuroprotective potential of new chromones isolated from the soil actinomycete Microbispora sp. TBRC6027
(2021-01-01)
Kittisrisopit, Suchada
;
Bunbamrung, Nantiya
;
Thawai, Chitti
;
Tadtong, Sarin
;
Niemhom, Nantawan
Nine new chromone analogs (1–9) were isolated from the soil actinomycete Microbispora sp. TBRC6027. The chemical structures were determined based upon NMR spectroscopic methods. These compounds were evaluated in vitro by using P19-derived neurons for neuroprotective activity against oxidative stress induced by serum deprivation and showed % viability of neurons at the concentration of 1 ng/mL varied from 43.51% to 52.99% without significant neurotoxicity for P19-derived neurons at the same concentration. Moreover, all tested compounds were inactive for antibacterial activity against both Gram-positive and Gram-negative bacteria and for cytotoxicity against MCF-7 (human breast cancer) and Vero cells at maximum tested concentration 50 μg/mL. However, compounds 4, 6, and 7 displayed weak cytotoxicity against NCI-H187 (human small-cell lung cancer) cells with IC<inf>50</inf> in a range of 87.99–91.57 μM.
Some of the metrics are blocked by your
consent settings
Microbispora soli sp. nov., isolated from soil of a hot spring
(2018-12-01)
Kittisrisopit, Suchada
;
Pittayakhajonwut, Pattama
;
Tadtong, Sarin
;
Thawai, Chitti
A novel actinomycete, strain RTBAU4-9 T , which produced longitudinally paired spores on aerial mycelia, was isolated from a soil sample of a hot spring area. This strain exhibited chemotaxonomic and genotypic properties typical of the genus Microbispora, such as meso-diaminopimelic acid in the cell-wall peptidoglycan, glucose, ribose and trace amount of madurose as the characteristic whole-cell sugars, phosphatidylethanolamine, diphosphatidylglycerol and phosphoglycolipids as the membrane phospholipids, MK-9(H <inf>4</inf> ), MK-9(H <inf>2</inf> ) and MK-9(H <inf>0</inf> ) as the characteristic menaquinones, and iso-C <inf>16: 0</inf> and anteiso-C <inf>17: 0</inf> as the main fatty acids. The G+C content of the genomic DNA was 70.2 mol%. The result of 16S rRNA gene sequence analysis revealed the strain was a member of the genus Microbispora and was most closely related to Microbispora hainanensis 211020 T (98.3% 16S rRNA gene sequence similarity). In addition, the low percentage of DNA-DNA relatedness (<28.1±1.2 %) and several phenotypic differences confirmed that strain RTBAU4-9 T should be considered as representing a novel species of the genus Microbispora, for which the name Microbispora soli sp. nov. is proposed. The type strain is RTBAU4-9 T (=TBRC 7648 T =NBRC 113147 T ).

KMITL

Browse

Filters

Settings

Sort By

Results per page

Search Results