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    Edible coating of chitosan ionically combined with κ-carrageenan maintains the bract and postharvest attributes of dragon fruit (Hylocereus undatus)
    (2021-08-01)
    H., Nguyen T.
    ;
    P., Boonyaritthongchai
    ;
    M., Buanong
    ;
    S., Supapvanich
    ;
    C., Wongs Aree
    Dragon fruit (Hylocereus undatus) has medicinal properties due to its rich antioxidant profile. Dragon fruit also has an attractive appearance of red peel and green bracts. However, shrivelling and weight loss, bract yellowing, and postharvest diseases are major challenges to the dragon fruit trade. The objective of the present work was, therefore, to formulate a coating composed of chitosan and κ-carrageenan for dragon fruits during storage at 10°C. The composite coating based on 1% chitosan (w/v) and 0.2% (w/v) κ-carrageenan with 0.75% (w/v) glycerol as a plasticiser effectively reduced the physiological weight loss and maintained the freshness of the dragon fruits, while increasing the phenolic content and maintaining the titratable acidity in the pulp. The composite coating delayed chlorophyll degradation by suppressing chlorophyllase and chlorophyll-degrading peroxidase, thereby maintaining the chlorophyll content (45.46 mg/100 g dry weight) and freshness of the bracts. However, the composite coating did not possess a strong effect on enhancing chitinase and β-1-3 glucanase activities of dragon fruits during storage and controlling disease symptoms.
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    Postharvest Hot Water Treatment Followed by Chitosan- and κ-Carrageenan-Based Composite Coating Induces the Disease Resistance and Preserves the Quality in Dragon Fruit (Hylocereus undatus)
    (2020-01-01)
    Nguyen, Hanh Thi
    ;
    Boonyaritthongchai, Panida
    ;
    Buanong, Mantana
    ;
    Supapvanich, Suriyan
    ;
    Wongs-Aree, Chalermchai
    Abstract: Postharvest diseases and loss of freshness are major problems that cause the deterioration of dragon fruit. The present study determined the effect of hot water treatment (HWT) at 50°C for 5 min followed by 1.0% chitosan and 0.2% κ-carrageenan-based composite coating to counter the issues. The results showed that HWT followed by the composite coating-enhanced antioxidant enzyme activities and reduced accumulation of H<inf>2</inf>O<inf>2</inf> in the peel, which demonstrated the defensive response in the tissues leading to reduce disease development. The chitinase and β-1,3-glucanase did not show a significant effect in reducing the postharvest diseases of dragon fruit. While the HWT helped to increase phenolics content and antioxidant capacity in the pulp, the composite coating was crucial to maintain green color of the bract. Therefore, we concluded that HWT combined with composite coating controlled the diseases via antioxidant defensive response and maintained the overall quality of dragon fruit for 30 days of storage at 10°C.
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    Molecular identification of chaetomium species from soil in Vietnam
    (2018-03-01)
    Quyet, N. T.
    ;
    Thanh, N. D.
    ;
    Cuong, H. V.
    ;
    Huong, B. T.L.
    ;
    Soytong, K.
    This study reported some species of the Chaetomiaceae from collected soil samples in Vietnam. Soil samples were taken from dragon fruit crop cultivation in Tien Giang and Long An provinces in 2017. All species of Chaetomiaceae were isolated from soil samples by soil baiting technique. All species were identified based on the morphological characteristics and phylogenetic analysis of β-tubulin gene. At least four species of the Chaetomiaceae family were identified, including Arcopilus aureus, Arcopilus cupreus, Chaetomium cochliodes and Chaetomium globosum. These strains were strongly antagonistic activity against Neoscytalidium dimidiatum causing brown spot disease of Hylocereus undatus (white-fleshed pitahaya) in Vietnam. Further research would be done the control mechanism and acute and dermal toxicology test for environmental safety. Finally it maybe formulate as biological fungicide to control this serious disease in the field.