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    Ornithine-decarboxylase activity assay during enrichment: Early identification of Escherichia coli O157:H7-free food samples
    (2024-01-01)
    Sangadkit, W.
    ;
    Kaewboonruang, S.
    ;
    Wattanachaisaereekul, S.
    Enrichment step is a cell amplification step essential for all detection of foodborne pathogens. The present work developed an early identification of finished food samples free from Escherichia coli O157:H7 contamination. Our work exploited the activity of ornithine decarboxylase (ODC) along with its pH indicator and a selective inhibitor. In the presence of the target bacteria, the enrichment broth changed colour significantly due to the diauxic growth during sugar fermentation and ODC activity. Among all conditions, phenol red and 0.028 g/L novobiocin were found to be optimal and selective for determining the presence or absence of E. coli O157:H7. When tested on real food samples, the proposed method yielded results that agreed well with the conventional method (Cohen’s kappa = 1.00). The present work provided a new method for quickly determining if finished food products are free from E. coli O157:H7 contamination.
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    Novel pineapple leaf fibre composites coating with antimicrobial compound as a potential food packaging
    (2022-01-01)
    Todhanakasem, Tatsaporn
    ;
    Panjapiyakul, Sorawit
    ;
    Koombhongse, Piyawit
    Biodegradable packaging coated with antimicrobial compounds has emerged as a new type of food packaging with potential to enhance food safety, extend shelf life and minimize plastic pollution. We have developed a polylactic acid (PLA) composite pineapple leaf fibre (PALF) biopolymer to fulfil the high demand of biodegradable materials for a potential use in food packaging. Our formulation with 10% pineapple fibre PLA/PALF composites exhibited enhanced melting temperatures and heat resistance, with a 44% reduction in the tensile strength and 2.7-fold reduction on the strain at the break when compared with neat PLA. To impart antimicrobial activity, the PLA/PALF was coated with crude supernatants isolated from Pediococcus pentosaceus PP04 containing 1.3% w/v of nisin. This material coated with the crude extract was shown to inhibit the growth of Bacillus cereus ATCC 11778, Salmonella enterica subsp. enterica serovar Typhimurium ATCC 13311, and Escherichia coli ATCC 8739 in culture conditions, as well as minimizing the number of the tested pathogens on coated material surfaces.
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