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    Astaxanthin producing yeasts: Production and genomic analysis
    (2024-09-16) ;
    Phuengjayaem, Sukanya
    ;
    Tanasupawat, Somboon
    This chapter explores yeasts and their potential in carotenoid production, delving into the diverse roles of carotenoids, their biosynthesis, and applications. Carotenoids, found in various organisms, serve several crucial functions, including light harvesting, photoprotection, and antioxidant activity. The focus shifts to astaxanthin, a red-orange carotenoid with immense market value and applications, notably in aquaculture and health. The astaxanthin-producing yeast strains emerge as a promising biotechnological platform for carotenoid production, particularly astaxanthin. Its unique features, FDA approval, and ability to synthesize carotenoids make it an ideal candidate for industrial applications. This chapter details the structure of astaxanthin and its biosynthetic pathway, emphasizing key genes involved. Additionally, it highlights other carotenoid producers and the biochemistry of astaxanthin. The latter part focuses on the health-promoting effects of astaxanthin, including antioxidant, anti-inflammatory, anti-diabetic, and anticancer activities. Immunomodulation benefits and the safety profile of astaxanthin are also discussed. The chapter concludes by addressing current trends, perspectives, and challenges in astaxanthin production, emphasizing the importance of circular, zero-waste processes.
      1
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    Hydrocarbon production and biodiesel properties of a green microalga Botryococcus braunii KMITL 2 cultivated outdoor in open pond and closed photobioreactor
    (2018-03-01)
    Botryococcus braunii is a promising feedstock for biodiesel production. In general, selection of the most suitable cultivation systems is based on profitable yield and oil quality. The feasibility of using this alga as feedstock for commercial biodiesel production was tested by comparing the cultivation in open pond and closed photobioreactor under the 5 g⋅kg<sup>-1</sup> optimum salinity condition previously found, and the results were that the hydrocarbon content (40.7±1.6%) of the alga cultivated in open pond was higher than that cultivated in the photobioreactor and its biomass (2.9±0.1 g⋅L<sup>-1</sup>) was much higher; its biodiesel properties, however, were nearly identical with cetane values of 48.15-56.99. Drastically different though were the hydrocarbon yields-cultivation in the open pond produced 2.3 times higher yield than the closed photobioreactor. Hence, outdoor cultivation under optimum salinity in open pond was considered suitable for biodiesel production by this algal strain.
      3
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    Item type:Publication,
    Enhanced growth and hydrocarbon production of Botryococcus braunii KMITL 2 by optimum carbon dioxide concentration and concentration-dependent effects on its biochemical composition and biodiesel properties
    (2017-01-01) ;
    Prachom, Noratat
    ;
    Sornchai, Piyanast
    The purposes of this study were to find the optimum level of supplementing CO<inf>2</inf> for increasing the biomass and hydrocarbon production of B. braunii KMITL 2 (A race) and to determine the effects of CO<inf>2</inf> level on CO<inf>2</inf> fixation and biodiesel properties. The experimental results showed that the alga supplemented with 10% CO<inf>2</inf> produced the highest biomass (1.48 ± 0.02 g L<sup>−1</sup>) and CO<inf>2</inf> fixation rate (100.43 ± 1.42 mg L<sup>−1</sup> d<sup>−1</sup>) that were 2.7 and 5.3 times higher than the control (0.04% CO<inf>2</inf>). The gravimetric hydrocarbon content (36.82 ± 3.39%) and hydrocarbon titer (0.35 ± 0.03 g L<sup>−1</sup>) of the alga supplemented with 5% CO<inf>2</inf> were 1.6 and 2.7 times higher than the control. Cultivation of this strain under 5% CO<inf>2</inf> gave the highest hydrocarbon yield and good biodiesel properties with the lowest iodine value and a higher CN value than the ASTM D6751 and EN 14214 fuel standards.