Ruangsomboon, Suneerat
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Ruangsomboon, Suneerat
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suneerat.ru@kmitl.ac.th
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Item type:Publication, Effect of light, nutrient, cultivation time and salinity on lipid production of newly isolated strain of the green microalga, Botryococcus braunii KMITL 2(2012-04-01)The green microalga strain, Botryococcus braunii KMITL 2, was isolated from a freshwater reservoir in central Thailand, and the effects of light, nitrogen, phosphorus, iron, cultivation time and salinity on lipid production were studied by varying parameters one at a time. When cultured in Chlorella medium containing 222mgL <sup>-1</sup> phosphorus (PO43--P) under continuous illumination of 200μEm <sup>-2</sup>s <sup>-1</sup> with a salinity of 0psu, a maximum lipid content of 54.69±3.13% was obtained. Its high lipid content makes strain KMITL 2 a potential source for biodiesel production in tropical regions. © 2011 Elsevier Ltd. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Enhancing biomass, hydrocarbon and biodiesel properties of green microalga Botryococcus braunii KMITL through gamma and UV radiation exposure(2024-12-01); This study investigated the effects of gamma (<sup>137</sup>Cs, 0–250 Gy) and UV (UV-C, 0–12 h) radiation on growth and biodiesel properties of Botryococcus braunii KMITL. For gamma radiation, maximum biomass (1.37 ± 0.02 g L<sup>−1</sup>) was achieved with 50 Gy, while a dose of 200 Gy resulted in the highest hydrocarbon content (51.84 ± 0.20%) and yield (0.66 ± 0.01 g L<sup>−1</sup>). For UV radiation, a 9 h exposure produced the highest biomass (2.45 ± 0.05 g L<sup>−1</sup>), hydrocarbon content (55.01 ± 1.22%), and yield (1.35 ± 0.04 g L<sup>−1</sup>). Algae exposed to gamma radiation within the range of 0–150 Gy exhibited C16:0 as the dominant fatty acid methyl ester (FAME), similar to those exposed to UV radiation, while algae exposed to 200–250 Gy displayed C18:1n9t as the dominant FAME. High levels of gamma and UV radiation were observed to lengthen fatty acid chains and increase unsaturated fatty acids. The cetane values of biodiesel from algae exposed to gamma and UV radiation ranged from 64.55 ± 0.14–66.47 ± 0.20 and 59.43 ± 0.04–65.27 ± 0.22, respectively, all meeting standard criteria. Both gamma and UV radiation also improved the saponification value and cold flow properties of the biodiesel. These findings suggest that controlled levels of gamma and UV radiation effectively enhance hydrocarbon yields with significant implications for biofuel production. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Hydrocarbon production and biodiesel properties of a green microalga Botryococcus braunii KMITL 2 cultivated outdoor in open pond and closed photobioreactor(2018-03-01)Botryococcus braunii is a promising feedstock for biodiesel production. In general, selection of the most suitable cultivation systems is based on profitable yield and oil quality. The feasibility of using this alga as feedstock for commercial biodiesel production was tested by comparing the cultivation in open pond and closed photobioreactor under the 5 g⋅kg<sup>-1</sup> optimum salinity condition previously found, and the results were that the hydrocarbon content (40.7±1.6%) of the alga cultivated in open pond was higher than that cultivated in the photobioreactor and its biomass (2.9±0.1 g⋅L<sup>-1</sup>) was much higher; its biodiesel properties, however, were nearly identical with cetane values of 48.15-56.99. Drastically different though were the hydrocarbon yields-cultivation in the open pond produced 2.3 times higher yield than the closed photobioreactor. Hence, outdoor cultivation under optimum salinity in open pond was considered suitable for biodiesel production by this algal strain. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Effects of different nitrogen, phosphorus, and iron concentrations and salinity on lipid production in newly isolated strain of the tropical green microalga, Scenedesmus dimorphus KMITL(2013-06-01); ; Choochote, SakchaiThe fatty acid composition, the effect of different concentrations of nitrogen (16.5-344 mg L<sup>-1</sup>), phosphorus (9-45 mg L<sup>-1</sup>), iron (9-45 mg L<sup>-1</sup>) and salinity levels (0-20 psu) on lipid production in the green microalga Scenedesmus dimorphus KMITL, a new strain isolated from a tropical country, Thailand, were studied. The alga was isolated from a freshwater fish pond, and cultured in Chlorella medium by varying one parameter at a time. The main fatty acid composition of this strain was C16-C18 (97.52 %) fatty acids. A high lipid content was observed in conditions of 16.5 mg L<sup>-1</sup>-N, or 22 mg L<sup>-1</sup>-P, or 45 mg L<sup>-1</sup>-Fe, or 5 psu salinity, which accumulated lipids to 20.3 ± 0.4, 19.4 ± 0.2, 24.7 ± 0.5, and 14.3 ± 0.2 % of algal biomass, respectively. Increasing lipid content and lipid productivity was noted when the alga was cultured under high iron concentration and high salinity, as well as under reduced phosphorus conditions, whereas nitrogen limitation only resulted in an increased lipid content. © 2012 Springer Science+Business Media Dordrecht. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Effect of media and salinity on lipid content of cyanobacterium Hapalosiphon sp(2014-01-01)The aim of the present work was to study the individual effect of media constituents: sodium nitrate, di-potassium hydrogen orthophosphate, ferric ammonium citrate and salinity on lipid production by Hapalosiphon sp. which cultured in BG-11 medium, in view of its possible utilization as novel raw materials for biodiesel production. Lipid content of Hapalosiphon sp. cultivated in medium containing 1, 500 mg/L sodium nitrate was 15. 78±0. 51%. Indeed, the highest lipid yield of 0. 063 g/L was found in a medium containing 375 mg/L sodium nitrate. The highest lipid content of Hapalosiphon sp. cultivated in medium containing 10 mg/L di-potassium hydrogen orthophosphate was 11. 50±0. 45%, while the highest lipid yield of 0. 044 g/L were occurred in a medium containing 20 mg/L di-potassium hydrogen orthophosphate. In various ferric ammonium citrate concentrations cultivation, the highest lipid content of Hapalosiphon sp. cultivated in a medium containing 9 mg/L ferric ammonium citrate was 12. 64±0. 38%. However, the highest lipid yield of 0. 063 g/L occurred in a medium containing 12 mg/L ferric ammonium citrate. The significantly highest lipid content and lipid productivity of Hapalosiphon sp. cultivated in salinity 10 ppt were 9. 95±0. 34% and 0. 049 g/L respectively. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Characterization and physicochemical properties of a novel microencapsulated bio-calcium from Asian sea bass bones(2025-12-01); ; ; Calcium is the most abundant mineral in the human body, yet intake remains insufficient in many populations. Fishbone-derived bio-calcium from Asian sea bass (Lates calcarifer), containing approximately 37.5 % calcium (dry weight), offers a cost-effective source. However, its primary form, hydroxyapatite, has low solubility due to high crystallinity, limiting its application in food fortification. This study aimed to enhance the physicochemical properties of bio-calcium (B) powders by encapsulating them with maltodextrin (M), gum arabic (G), and their combination (MG) at 5 %, 10 %, and 15 % (w/v) using spray drying. A 1:4 (w/w) ratio of B to wall materials was applied at 180 °C (inlet) and 60 °C (outlet) temperatures. Powder yields ranged from 25.2 % (15 % BG) to 30.3 % (15 % BM), with no significant differences (p > 0.05) among treatments. Encapsulated powders had higher lightness (L*) than B. The highest calcium content and encapsulation efficiency were observed in 5 % BG, while BM showed the lowest. Moisture content and water activity remained below 10 % and 0.6 %, respectively. BG had the highest hygroscopicity, while wall concentration had no significant (p > 0.05) impact. Encapsulation improved water solubility index (75.4–86.5 %), especially in BM. Particle sizes ranged from 0.92 µm (10 % BMG) to 2.89 µm (15 % BM), while zeta potentials ranged from -8.71 mV (15 % BM) to -20.90 mV (15 % BMG). Encapsulated powders were more spherical and smoother than B, while BG particles showed aggregation, whereas BMG showed mixed morphologies. These findings suggest that encapsulation enhanced the physicochemical properties of bio-calcium, supporting its potential application in calcium-fortified foods and dietary supplements. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Bioaccumulation of cadmium in an experimental aquatic food chain involving phytoplankton (Chlorella vulgaris), zooplankton (Moina macrocopa), and the predatory catfish Clarias macrocephalus × C. gariepinus(2006-06-10); Wongrat, LaddaThe accumulation of cadmium (Cd) was studied in an experimental aquatic food chain involving the phytoplankton Chlorella vulgaris as the primary producer, the zooplankton Moina macrocopa as the primary consumer, and the catfish Clarias macrocephalus × Clarias gariepinus as the secondary consumer. C. vulgaris was first exposed to Cd solutions at 0.00, 0.35, and 3.50 mg l<sup>-1</sup>, referred to as control group and experimental groups 1 and 2, respectively. Subsequently, each group was fed to three corresponding groups of M. macrocopa. Finally, three groups of catfish were fed these corresponding groups of M. macrocopa. After C. vulgaris was exposed to 3.50 mg l<sup>-1</sup> Cd (experimental group 2), the residual Cd in solution was only 4.01 μg l<sup>-1</sup>, lower than the maximum allowable limit of Cd in natural water sources (5 μg l<sup>-1</sup>). Cd concentrations in C. vulgaris were 0.01 ± 0.00 μg g<sup>-1</sup> (dry wt) in the control group, 194 ± 1.80 μg g<sup>-1</sup> (dry wt) in experimental group 1, and 1140 ± 20.06 μg g<sup>-1</sup> (dry wt) in experimental group 2. The Cd concentrations in M. macrocopa were 0.01 ± 0.00 μg g<sup>-1</sup> (dry wt) in the control group, 16.48 ± 2.23 μg g<sup>-1</sup> (dry wt) in experimental group 1, and 56.6 ± 3.23 μg g<sup>-1</sup> (dry wt) in experimental group 2. The Cd concentrations in catfish muscle increased with increasing Cd concentrations in the food. After 60 days of fish culture, the mean concentrations of Cd in fish muscle were 0.01 ± 0.00 μg g<sup>-1</sup> (dry wt) in the control group, 0.61 ± 0.02 μg g<sup>-1</sup> (dry wt) in experimental group 1 and 1.04 ± 0.06 μg g<sup>-1</sup> (dry wt) in experimental group 2. Cd concentration in fish muscle of experimental group 2 was equal to the permissible limit. Cd accumulation affected fish growth: at the end of the study, the mean fresh weight (12.81 g) of catfish in the control group, was significantly higher than those experimental group 1 (10.43 g) and experimental group 2 (10.00 g). The results showed that the measurement of Cd concentration in water does not necessarily give a measure of the safety of aquatic organisms as human food. Hence, heavy metal contamination is a matter for concern when organisms are harvested, for fish and human consumption, from natural water sources. © 2006 Elsevier B.V. All rights reserved. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Enhanced growth and hydrocarbon production of Botryococcus braunii KMITL 2 by optimum carbon dioxide concentration and concentration-dependent effects on its biochemical composition and biodiesel properties(2017-01-01); - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Effects of different media and nitrogen sources and levels on growth and lipid of green microalga Botryococcus braunii KMITL and its biodiesel properties based on fatty acid composition(2015-06-08)This work aimed to find an optimum culture medium for green microalga Botryococcus braunii KMITL and investigate its biodiesel properties based on fatty acid composition. Four different media were tested. Chlorella medium was the best medium for lipid yield. Among four nitrogen sources tested, KNO<inf>3</inf> produced the highest lipid yield. When varied the nitrogen concentrations, this strain gave the highest lipid yield at the highest nitrogen level. When cultivated in the best medium and nitrogen source and level for 30 days, and then cultivated further for 14 days in the medium with no nitrogen, the highest lipid content and yield were 49.94 ± 0.82% and 2.71 ± 0.02 g L<sup>-1</sup>, respectively. C16:0 fatty acid was the major fatty acid found. Fatty acid profiles of B. braunii KMITL cultivated in Chlorella medium with 1.25 g L<sup>-1</sup> KNO<inf>3</inf> gave the best biodiesel properties with the lowest iodine value, maximum cetane number, and lowest degree of unsaturation. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Cyanobacterial composition and microcystin accumulation in catfish pond(2014-01-01);