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    Identification of new position of DNA insertion and 24 bp INDEL mutation polymorphism in Prolactin gene promoter of Thai native chickens
    (2025-07-01)
    Thongsaiklaing, T.
    ;
    Datumada, H.
    ;
    Morris, J.
    ;
    This research identified a 24-bp INDEL (-358) on the promoter region of the PRL gene in Thai native chickens. The allele frequencies for allele I and allele D were 0.20 and 0.80, respectively. The genotype frequencies were II (0.028), ID (0.349), and DD (0.622), respectively. The heterozygosity values comprised the Ho (0.350), higher than the He value (0.323). Moreover, our findings revealed new DNA duplication on the PRL promoter region, with three distinct polymorphisms of 30 bp (-358), 24 bp (-358), and 14 bp (-358), respectively.
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    Item type:Publication,
    Ectoparasite species attacking chicken in eastern area of Bangkok, Thailand
    (2022-07-01)
    Lakyat, A.
    ;
    Pumnuan, J.
    ;
    Thipmanee, K.
    Ectoparasites of chickens are important for poultry farming and other types of poultry rearing by ways of causing nuisance and being as transmitter of various diseases to poultry resulting in poor-quality and quantity products. From the directly survey result, it was found that Megninia cubitalis mostly attacked laying hens reared in a cage at neck, wing, chest, back and buttock with the average number of 52.1- 204.9 mites per chicken, and followed by injured on Rhode Island red with 85.0 mites, abundantly at buttock. And the louse, Lipeurus caponis was found in Polish chicken with 100.5 insects that mostly appeared at back of chicken, and followed by the appearance in Rhode Island red with 70.0 insects, abundantly at chest. This insect preferred to live at wing and chest. Whereas, result from suction method showed that Megninia cubitalis was also found in laying hen reared in a cage with the average number of 71.4-168.3 mites per chicken. It lives everywhere on the body as neck, head and buttock. Besides, Cuclotogaster heterogoraphus was observed on the body area equal to 43.8 insects, where the louse, Menopon gallinae was monitored in Polish chickens with totally, 22.5 insects, plenty at the buttock area.
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    Properties changes of chicken breast during sous-vide cooking and acceptance for elderly
    (2019-01-01) ;
    Suraphantapisit, Nuttapon
    ;
    Pongpaew, Possathorn
    ;
    Srikalong, Pramual
    The sous-vide process has been used in cooking to maintain nutrients and improve texture of meat. This improvement in nutrients and softening of the texture could be advantageous for the elderly. The objective of this research was to study effects of sous-vide cooking on the properties and changes of chicken breast fillet and their sensory acceptance by the elderly. Chicken breast fillets were vacuum packed and cooked at 60 °C for different times from 1 to 8 h comparing the effects with a common way of cooking as a control (100 °C for 30 min). Sous-vide cooked chicken had significantly (P < 0.05) higher yield percentage, water holding capacity (WHC) and redness and lower hardness, chewiness and lightness than the control. With increased sous-vide cooking time the yield percentage, WHC, redness and hardness significantly (P < 0.05) decreased, while lightness and chewiness significantly increased (P < 0.05). Sous-vide cooking for 3, 4 and 5 h resulted in the lowest hardness, which was in the range of 40.05-60.01 N and lowest chewiness in the range of 11.24-16.88, but the highest springiness in the range of 0.03-0.04. A sensory evaluation panel of 50 people of 60-70 years old tested samples of the sous-vide breast chicken after cooking for 3, 4 or 5 h. Their evaluation showed that cooking for 4 h had the highest acceptance score compared with other times.
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    Item type:Publication,
    Chemical compositions of eggs from chicken, quail and snail-eating turtle
    (2011-12-01)
    Tunsaringkarn, T.
    ;
    Siriwong, W.
    ;
    Tungjaroenchai, W.
    This study was aimed to evaluate chemical compositions, profiles of amino acids and fatty acids, vitamin and sex hormone contents of eggs from chicken, quail and snail-eating turtle, respectively. Whole quail egg had higher levels of fat (9.89 g 100g<sup>-1</sup>), and energy (156.50 kcal 100g<sup>-1</sup>) than the others. Snail-eating turtle egg contained the highest levels of total solids (5.36%) and ash (1.45 g 100g<sup>-1</sup>). Total of amino acids content of either quail or chicken egg white (10.78 or 9.33 g 100g<sup>-1</sup>) was higher than that of snail-eating turtle. Ratio of unsaturated and saturated fatty acids content found the highest in snail-eating turtle eggs. Quail egg yolks contained the highest content of vitamin A (retinol) and D (717.00 and 1.14 μg 100g<sup>-1</sup>), while the chicken egg contained the highest level of vitamin E (6.37 mg 100g<sup>-1</sup>). Most of sex hormones content; Luteinizing Hormone (LH), Follicle Stimulating Hormone (FSH), Prolactin (PRL), Progesterone (P), Estradiol (E2) and Testosterone (T), were high in egg yolks. Whole eggs of chicken egg contained the highest content of hormone P (807.06 ng g<sup>-1</sup>) which was higher than that of quail or snail-eating turtle (314.83 or 157.36 ng g<sup>-1</sup>). This study indicated that quail egg contained higher contents of amino acid, such as aspartic acid and leucine, vitamin A and D, than the others. While, the chicken egg contained higher levels of vitamin E and sex hormones P than the others. Total minerals were the most abundant in snail-eating turtle egg.
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    Purification and amino acid sequence of a bacteriocins produced by Lactobacillus salivarius K7 isolated from chicken intestine
    (2006-01-01) ;
    Sakpuaram, Thavajchai
    ;
    Wajjwalku, Worawidh
    ;
    Nitisinprasert, Sunee
    ;
    Swetwiwathana, Adisorn
    A bacteriocin-producing strain, Lactobacillus K7, was isolated from a chicken intestine. The inhibitory activity was determined by spot-on-lawn technique. Identification of the strain was performed by morphological, biochemical (API 50 CH kit) and molecular genetic (16S rDNA) basis. Bacteriocin purification processes were carried out by amberlite adsorption, cation exchange and reverse-phase high perform ance liquid chromatography. N-terminal amino acid sequences were performed by Edman degradation. Molecular mass was determined by electrospray-ionization (ESI) mass spectrometry (MS). Lactobacillus K7 showed inhibitory activity against Lactobacillus sakei subsp. sakei JCM 1157<sup>T</sup>, Leuconostoc mesenteroides subsp. mesenteroides JCM 6124<sup>T</sup> and Bacillus coagulons JCM 2257<sup>T</sup>. This strain was identified as Lb. salivarius. The antimicrobial substance was destroyed by proteolytic enzymes, indicating its proteinaceous structure designated as a bacteriocin type. The purification of bacteriocin by amberlite adsorption, cation exchange, and reverse-phase chromatography resulted in only one single active peak, which was designated FK22. Molecular weight of this fraction was 4331.70 Da. By amino acid sequence, this peptide was homology to Abp 118 beta produced by Lb. salivarius UCC118. In addition, Lb. salivarius UCC118 produced 2-peptide bacteriocin, which was Abp 118 alpha and beta. Based on the partial amino acid sequences of Abp 118 beta, specific primers were designed from nucleotide sequences according to data from GenBank. The result showed that the deduced peptide was high homology to 2-peptide bacteriocin, Abp 118 alpha and beta.
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