Passara, Hataichanok

Preferred name

Alternative Name

Passara, H.

Main Affiliation

School of Agricultural Technology

hataichanok.pa@kmitl.ac.th

Now showing 1 - 10 of 25

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Adulticidal synergy of two plant essential oils and their major constituents against the housefly Musca domestica and bioassay on non-target species
(2024-03-15)
Soonwera, Mayura
;
Moungthipmalai, Tanapoom
;
Puwanard, Cheepchanok
;
Sittichok, Sirawut
;
Sinthusiri, Jirisuda
Single and mixture formulations of lemongrass (Cymbopogon citratus (DC.) Stapf.) and star anise (Illicium verum (J. Presl.)) essential oils (EOs) and their major constituents were assayed for their adulticidal activities against housefly, Musca domestica L., and two non-target species, stingless bee (Tetragonula pegdeni Schwarz) and guppy (Poecilia reticulata Peters). The efficacies of the mixture formulations were compared against those of the single formulations and 1.0% α-cypermethrin, a common synthetic insecticide. GC-MS analysis found that the major constituent of lemongrass EO was geranial (45.23%), and that of star anise EO was trans-anethole (93.23%). Almost all mixture formulations were more effective in adulticidal activity against housefly adults than single formulations and 1.0% α-cypermethrin. A mixture of 1.0% lemongrass EO + 1.0% trans-anethole exhibited the strongest synergistic insecticidal activity with a 100% mortality rate (KT<inf>50</inf> of 3.2 min and LT<inf>50</inf> of 0.07 h). The relative percentage increase in mortality rate over single formulations was between 1.6 and 91.9%. In addition, it was three times more effective than 1.0% α-cypermethrin. To find the mechanism of adulticidal action, scanning electron microscopy (SEM) was done to find morphological aberrations, such as antennal and mouthpart aberrations, after the houseflies were treated with 1.0% lemongrass EO + 1.0% trans-anethole. The aberrations included deformed and abnormal shape of arista and flagellum, change in labellum pigmentation, and damage to pseudotracheae. Regarding toxicity against non-target species, all single and mixture formulations were not toxic to the two non-target species, while 1.0% α-cypermethrin was highly toxic. To conclude, a mixture of 1.0% lemongrass EO + 1.0% trans-anethole can be an excellent, natural, sustainable housefly adulticidal agent.
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Identification of new position of DNA insertion and 24 bp INDEL mutation polymorphism in Prolactin gene promoter of Thai native chickens
(2025-07-01)
Thongsaiklaing, T.
;
Datumada, H.
;
Morris, J.
;
Passara, H.
This research identified a 24-bp INDEL (-358) on the promoter region of the PRL gene in Thai native chickens. The allele frequencies for allele I and allele D were 0.20 and 0.80, respectively. The genotype frequencies were II (0.028), ID (0.349), and DD (0.622), respectively. The heterozygosity values comprised the Ho (0.350), higher than the He value (0.323). Moreover, our findings revealed new DNA duplication on the PRL promoter region, with three distinct polymorphisms of 30 bp (-358), 24 bp (-358), and 14 bp (-358), respectively.
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Antifungal efficacy of stingless bee honey extracts against a black mould causing pathogen Lasiodiplodia spp
(2026-03-01)
Datumada, H.
;
Niyomdecha, A.
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Passara, H.
;
Ramdoung, T.
;
Sudwisai, W.
Fungal species causing the black sooty mould were isolated from fruit peels and subjected to molecular genetic analysis. The nucleotide sequences were found to be 100% identical to the fungus Lasiodiplodia iranensis. Results from testing the antifungal properties of stingless bee honey extracts showed that the extract from the H. itama species exhibited the highest inhibitory effect, followed by T. pagdeni, G. thoracica, and T. fuscobalteata. The average inhibition values were 21.43+1.27, 18.54+1.50, 17.51+1.76, and 17.18+0.91 mm., respectively.
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Anise and Fennel Essential Oils and Their Combination as Natural and Safe Housefly Repellents
(2025-01-01)
Passara, Hataichanok
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Sittichok, Sirawut
;
Puwanard, Cheepchanok
;
Sinthusiri, Jirisuda
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Moungthipmalai, Tanapoom
Essential oils (EOs) are effective repellents and eco-friendly alternatives. We tested single and combination EOs of fennel and anise for repellency and stability against houseflies. All treatments were stored at 27 °C for up to 360 days. Efficacies were compared against α-cypermethrin as a reference. Safety bioassay used on two representative non-target species (guppies and earthworms) confirmed safety. The strongest repellency and stability, 100% repellency and RC<inf>50</inf> of 0.4 mL/m3 on day 1, was achieved by a fennel + anise EO combination (1:1). After 360 days, these combinations remained effective (95% repellency), and RC<inf>50</inf> = 0.8 mL/m3. The EO combinations were more effective than all single EOs and α-cypermethrin at all times, showing strong synergy with a synergistic repellent index of 2.4 to 3.3. This fennel + anise EO combination was more than 24 times more effective as a repellent than α-cypermethrin. Morphological damage included damaged antennae with twisted flagella and abnormal aristae. All single and combination EOs were not toxic to the non-targets and could be considered safe, whereas α-cypermethrin was highly toxic to them. Thus, the fennel + anise EO combination has great potential to be developed as a safe, natural repellent for managing housefly populations.
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Larvicidal efficacy and morphological abnormalities induced by plant essential oils against housefly (Musca domestica L.)
(2026-03-01)
Passara, H.
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Sittichok, S.
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Moungthipmalai, T.
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Laosinwattana, C.
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Thongsaiklaing, M.
The results showed that the lowest complete pupation rate was 43.67%, indicating a significant inhibition of normal metamorphosis. Additionally, 56.33% of third-instar larvae were classified as malformed larvae (ML), dying before pupation and exhibiting severe morphological and physiological abnormalities. These findings suggest that the star anise nanoemulsion interferes with key developmental pathways, preventing larvae from reaching the pupal stage. Due to its high effectiveness and plant-based origin, this nanoformulation offers a promising, environmentally friendly alternative to conventional chemical insecticides. It has potential for further development into a natural larvicidal agent for use in integrated pest management (IPM) strategies targeting housefly populations. Future research should explore the underlying mechanisms of action and assess the efficacy and safety of this nanoemulsion under real-world conditions to support its application in sustainable vector control.
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Efficacy of plant essential oils for repelling against American cockroach adults (Periplaneta americana L.)
(2025-01-01)
Passara, H.
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Soonwera, M.
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Arhamad-Armeen, N.
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Sittichok, S.
;
Jintanasirinurak, S.
The combination of four plant essential oils (EOs) from star anise, citronella grass, lemon grass, and cinnamon was evaluated for repelling American cockroaches by the dual-choice method. A combination of star anise + citronella grass had a repellency rate of 95%. In contrast, combinations of star anise + lemon grass and star anise + cinnamon were effective between 73% and 69%, but the synthetic chemical repellent (12% DEET) was less effective at 59%. Therefore, the essential oils formulae had a high potential for effective use and, further, are harmless for healthiness and the environment. Thus, they can replace more toxic synthetic insecticides.
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Larvicidal and pupicidal activity of combination of two plant essential oils against Aedes aegypti
(2025-01-01)
Sinthusiri, J.
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Puwanard, C.
;
Passara, H.
;
Soonwera, M.
The larvicidal and pupicidal effects of six mixtures of cinnamon (Cinnamomum verum J. Presl.) and nutmeg (Myristica fragrans Houtt.) essential oils (EOs) and major compositions (geranial, α-pinene and trans- cinnamaldehyde) were tested against early fourth instar and pupal stages of Aedes aegypti. The combinations (2% cinnamon EO + 1% geranial, 2% cinnamon EO + 1% trans-cinnamaldehyde and 2% cinnamon EO + 1% α-pinene) showed high toxicity against early 4th instar larvae, with 100% mortalities at 24 hours and LT50 between 1.4 and 2.4 hours. Additionally, 2% nutmeg EO + 1% α-pinene showed >99% mortality at 24 hours with LT50 = 4.6 hours. 2% nutmeg EO + 1% geranial and 2% nutmeg EO + 1% α-pinene were highly toxic against pupae, with 100% mortality at 48 hours and LT50 values of 5.8 and 7.8 hours. On the other hand, temephos showed LT50 for larval and pupal stages at 9.8 and 94.1 hours. It showed that 2% nutmeg EO + 1% α-pinene was able to control immature stages and was more effective than temephos. However nutmeg extracts need to be checked for human and valuable species toxicity.
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Synergistic Larvicidal and Pupicidal Effects of Monoterpene Mixtures Against Aedes aegypti with Low Toxicity to Guppies and Honeybees
(2025-07-01)
Sittichok, Sirawut
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Passara, Hataichanok
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Moungthipmalai, Tanapoom
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Sinthusiri, Jirisuda
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Murata, Kouhei
The present study evaluated the larvicidal and pupicidal activities of pure and mixed monoterpene formulations—eucalyptol, geranial, trans-anethole, and trans-cinnamaldehyde—against Aedes aegypti and compared them with 1% (w/w) temephos. Safety bioassays of all formulations on non-target species confirmed their safety. The combined mixture of eucalyptol + trans-anethole at 400 µg/mL exhibited stronger larvicidal activity, with an LC<inf>50</inf> of 176 µg/mL, while the combination of trans-anethole + geranial at 400 µg/mL exhibited stronger pupicidal activity with an LC<inf>50</inf> of 167 µg/mL. Both formulations were more effective than a 1% temephos. All the mixture formulations were more strongly synergistic compared to pure formulations, with an increased mortality value (IMV) of 25% to 95%. External morphological aberrations observed at death included swelling of the respiratory system. Importantly, all the formulations were safe for two non-target species: guppies (Poecilia reticulata) and honeybees (Apis mellifera). The combination formulations are strong larvicides and pupicides for controlling Ae. Aegypti, which will help reduce the spread of viruses carried by this vector.
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Ovicidal Toxicity and Morphological Changes in Housefly Eggs Induced by the Essential Oils of Star Anise and Lemongrass and Their Main Constituents
(2024-07-01)
Passara, Hataichanok
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Sittichok, Sirawut
;
Sinthusiri, Jirisuda
;
Moungthipmalai, Tanapoom
;
Puwanard, Cheepchanok
This study attempted to evaluate the ovicidal activity of single-component formulations and combination formulations of lemongrass and star anise essential oils (EOs) and their main constituents against housefly eggs. The efficacies of the combinations were compared with those of single-component formulations and α-cypermethrin. Safety bioassays of all treatments and α-cypermethrin on non-target predators—guppy and molly—were conducted. Two combinations: 1% lemongrass EO + 1% trans-anethole and 1% star anise EO + 1% geranial, exhibited a strong ovicidal activity with an inhibition rate of 94.4 to 96.2%. They were 1.1 times as effective as α-cypermethrin. The two combinations also showed high synergistic activity compared to single-component formulations, with a high synergistic index and a high increased inhibition value of 37.4 to 57.7%. All EO treatments were benign for all non-target aquatic species with a high 50% lethal time (LT<inf>50</inf>) and safety index. In contrast, α-cypermethrin was highly toxic to them with a low LT<inf>50</inf>. The morphological abnormalities observed in housefly eggs at death were those such as the shrivelling of the eggs, aberrations and damage to the eggshells, hatching lines, aeropyles, plastron, and micropyle. The potential of these two combinations as a cypermethrin replacement is compelling.
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Evaluating the acaricidal effect of essential oil nanoemulsion against the cutworm, Spodoptera litura
(2022-09-01)
Passara, H.
;
Pumnaun, J.
;
Thipmanee, K.
The cutworm, Spodoptera litura is an important pest of cruciferous vegetables and it causes economic impact worldwide. The efficiency of turmeric and star anise essential oil nanoemulsions (nEOs) against the cutworm, including mortality, antifeedant, and growth inhibition effects was investigated. Essential oil nanoemulsions (nEOs) were prepared by mixing the essential oils with surfactants and co-surfactants. The 2nd, stage instar larvae of cutworms were tested by leaf dipping method and were observed after 24 hours. The results showed that the two nEOs, which were turmeric and star anise, performing the mortality, and growth development effect on Spodoptera litura as well as having antifeedant property. Complete mortality rate was shown at 0.35 % concentration of turmeric and star anise nEOs, when 100% antifeedant effect was found at 0.20% concentrations of nEOs, and 100% growth inhibition was observed at 0.30% concentrations of nEOs. It could be concluded that the nEOs from star anise had antifeedant and insecticide properties.
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Passara, Hataichanok

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