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    Item type:Publication,
    A New Species of Triaenodes (Trichoptera: Leptoceridae), based on morphological and molecular data, from Kaeng Khoi Waterfall, Chumphon Province, southern Thailand
    (2025-02-28)
    Laudee, Pongsak
    ;
    Malicky, Hans
    ;
    Takenaka, Masaki
    ;
    Sawangarrom, Phuwadon
    ;
    Suwannarat, Nannaphat
    A new species of Triaenodes tipmaneei sp. nov. is described and illustrated based on the male and female genitalia. The mitochondrial DNA (mtDNA) cytochrome c oxidase subunit I (COI) of the new species and of the most similar species T. themis Malicky and Prommi 2006 were analyzed. The male genitalia of Triaenodes tipmaneei sp. nov. is distinguished from other species in the genus by the shape of inferior appendages. Inferior appendages of the new species are without recurved processes; the dorsal branch is divided into a dorsal lobe and two processes, with the upper process curved upward and bifid, and the lower process slender and with pointed apex; the main body of the appendage has a short slender apicolateral process, the mesal process is short and pointed, the ventral process is stout, and the apex is pointed. The genetic distance between T. tipmaneei sp. nov. and T. themis, based on the mtDNA COI region, was 8.5 precent, indicating a large genetic differentiation between the two species.
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    Tracing the evolution and economic potential of konjac glucomannan in Amorphophallus species (Araceae) using molecular phylogeny and RAPD markers
    (2016-10-27)
    Mekkerdchoo, Orachorn
    ;
    Borompichaichartkul, Chaleeda
    ;
    Perrigo, Allison L.
    ;
    Srzednicki, George
    ;
    Prakitchaiwattana, Cheunjit
    The genus Amorphophallus is an economically important taxon that is abundant in Old World tropical forests (Asia, Oceania and Africa). It includes many species that are used with increasing frequency as a source of food and pharmaceutical products worldwide. Amorphophallus konjac is an important economic crop and has been used widely in China and Japan for commercial konjac glucomannan (KGM) production. However, the species’ range does not extend to Thailand, where other closely related species may be more suitable for commercial KGM production. Present understanding of genetic relaionships among Thai Amorphophallus species is still limited, and the connection between evolutionary history and KGM content is unknown. Here, the genetic relationships among various accessions of Amorphophallus spp. collected in Thailand are investigated using the chloroplast trnL-trnF spacer, nuclear ribosomal internal transcribed spacer (ITS) region and the second intron of LEAFY (FLint2) together with genome-wide DNA variation analysis, Randomly Amplified Polymorphic DNA (RAPD) technique. RAPD primers are also developed to quickly and efficiently identify species producing high levels of KGM. This study finds that two monophyletic clades include high KGM content species. RAPD analyses indicate that primer AC-10 generates specific bands identifying species belonging only to the high and medium KGM content clades. These primers can be used as a screening tool for economical species, aiming at improving the industrial production of KGM in Thailand and the world.
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