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Overview of biorefinery
(2022-01-01)
Thongchul, Nuttha
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Charoensuppanimit, Pongtorn
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Anantpinijwatna, Amata
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Gani, Rafiqul
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Assabumrungrat, Suttichai
A strong reliance on fossil resources gives rise to a depletion of nonrenewable resources and negative or harmful environmental impacts. Circumvention of this energy-environment nexus has been proposed through the application of the concept of biorefinery. In this concept, biomass, an alternative renewable feedstock containing C-rich chemicals, is utilized as a replacement of the fossil-based feedstock to produce bioenergy and bio-based chemicals. Originally, biorefinery was perceived as a platform of biomass processing, which would produce primarily fuels and chemicals. To date, biorefinery harnesses a variety of sustainable and synergetic technologies that converts biomass into a wide range of profitable products such as food-and-feed for the future, biopharmaceuticals, and nutraceuticals. Due to variability of feedstock and newly emerged technologies, classifications of biorefinery are diverse and depend on the basis (e.g., source of a biomass, the generation of a feedstock, etc.) taken in consideration. A comprehensive view of biorefinery requires the consideration of processing of biomass from different origins via diversified technology platforms. Since the concept of biorefinery also concerns social aspects and location-specific technologies, various aspects of stakeholders including academia, industry, economy, and society need also to be considered. Collaboration among the various actors is facilitated if necessary key information is easily accessible. Therefore, an overview of biorefinery should cover key information related to biorefinery, such as nature of biomass, current situation, available technologies, process design methods, associated tools, and analyses of processing routes along with case studies. In this chapter, the indices representing the key information related to biorefinery are arranged alphabetically and tabulated to enhance a good understanding of the concept of biorefinery.
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A review of organic waste enrichment for inducing palatability of black soldier fly larvae: Wastes to valuable resources
(2020-12-01)
Raksasat, Ratchaprapa
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Lim, Jun Wei
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Kiatkittipong, Worapon
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Kiatkittipong, Kunlanan
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Ho, Yeek Chia
The increase of annual organic wastes generated worldwide has become a major problem for many countries since the mismanagement could bring about negative effects on the environment besides, being costly for an innocuous disposal. Recently, insect larvae have been investigated to valorize organic wastes. This entomoremediation approach is rising from the ability of the insect larvae to convert organic wastes into its biomass via assimilation process as catapulted by the natural demand to complete its lifecycle. Among the insect species, black soldier fly or Hermetia illucens is widely researched since the larvae can grow in various environments while being saprophagous in nature. Even though black soldier fly larvae (BSFL) can ingest various decay materials, some organic wastes such as sewage sludge or lignocellulosic wastes such as waste coconut endosperm are destitute of decent nutrients that could retard the BSFL growth. Hence, blending with nutrient-rich low-cost substrates such as palm kernel expeller, soybean curd residue, etc. is employed to fortify the nutritional contents of larval feeding substrates prior to administering to the BSFL. Alternatively, microbial fermentation can be adopted to breakdown the lignocellulosic wastes, exuding essential nutrients for growing BSFL. Upon reaching maturity, the BSFL can be harvested to serve as the protein and lipid feedstock. The larval protein can be made into insect meal for farmed animals, whilst the lipid source could be extracted and transesterified into larval biodiesel to cushion the global energy demands. Henceforth, this review presents the influence of various organic wastes introduced to feed BSFL, targeting to reduce wastes and producing biochemicals from mature larvae through entomoremediation. Modification of recalcitrant organic wastes via fermentation processes is also unveiled to ameliorate the BSFL growth. Lastly, the sustainable applications of harvested BSFL biomass are as well covered together with the immediate shortcomings that entail further researches.
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Purification, physicochemical properties, and statistical optimization of fibrinolytic enzymes especially from fermented foods: A comprehensive review
(2020-11-15)
Moula Ali, Ali Muhammed
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Bavisetty, Sri Charan Bindu
Fibrinolytic enzymes are proteases responsible for cleavage of fibrin mesh in thrombus clots, which are the primary causative agents in cardiovascular diseases. Developing safe, effective and cheap thrombolytic agents are important for prevention and cure of thrombosis. Although a wide variety of sources have been discovered for fibrinolytic enzymes, only few of them have been employed in clinical and therapeutic applications due to the drawbacks such as high cost of production, low stability of enzyme or therapeutic side effects. However, the discovery of new fibrinolytic enzymes requires complex purification stages and characterization, which gives an insight into their diverse modes of action. Post-discovery, approaches such as a) statistical optimization for fermentative bioprocessing and b) genetic engineering are advantageous in providing economic viability by finding simple and cost-effective medium, strain development with sufficient nutrient supplements for stable and high-level production of recombinant enzyme. This review provides a comprehensive understanding of different sources, purification techniques, production through genetic engineering approaches and statistical optimization of fermentation parameters as proteases have a wide variety of industrial and biotechnological applications making 60% of total enzyme market worldwide. New strategies targeting increased enzyme yields, non-denaturing environments, improved stability, enzyme activity and strain improvement have been discussed.
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Study of ethanol fermentation reaction using Saccharomyces diastaticus in a two-tank fermentation system with cell recycling
(2018-10-01)
Lerkkasemsan, Nuttapol
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Lee, Wen Chien
Experimental data of ethanol fermentation in a two-tank system with cell recycling using sucrose as a substrate were investigated to establish a kinetic model that described the reaction. Flocculent yeast Saccharomyces diastaticus LORRE-316 was used as the fermenting yeast, and the fermentation medium comprised 80 g/L sucrose, 10 g/L yeast extract, 10 g/L peptone, 2 g/L potassium phosphate monobasic (KH<inf>2</inf>PO<inf>4</inf>), and 0.5 g/L magnesium sulfate heptahydrate (MgSO<inf>4</inf>·7H<inf>2</inf>O). Three models, the Monod model, the modified Monod model, and the extended-modified Monod model, were used to describe fermentation, and the extended-modified Monod model described the reaction more accurately than the other two models. The model took substrate limitations plus substrate and ethanol inhibitive effects into consideration, and was modified to include assumptions that included terms for substrates (sucrose, glucose and fructose) and product (ethanol). In addition, the ethanol concentration had a significant effect on cell growth. The results of Lineweaver–Burk plots showed that the maximum specific growth rate (μ<inf>MAX</inf>) and the Monod constant (K<inf>S</inf>) were 0.72/h and 26.77 g/L, respectively. The models were suitable for describing the experimental data.
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In vitro fermentation, digestibility and methane production as influenced by Delonix regia seed meal containing tannins and saponins
(2017-01-01)
Supapong, C.
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Cherdthong, A.
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Seankamsorn, A.
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Khonkhaeng, B.
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Wanapat, M.
The aim of the study was to evaluate the effect of supplementation of Delonix regia (DR) seed meal containing tannins and saponins on gas kinetics, ammonia-nitrogen (NH3-N) content, pH, methane (CH4) production and dry matter (DM) digestibility using an in vitro gas production technique. The experimental design was completely randomized, and the dietary treatments included DR seed meal supplementation at levels of 0, 3.3, 5.0, 6.7, 8.3, 10, 11.7, 13.3, 15.0 and 16.7 mg DM added to 0.5 g of roughage and concentrate (70:30) mixture. The gas production was measured at several time points: 0, 0.5, 1, 2, 4, 6, 8, 12, 18, 24, 48, 72 and 96 h by a pressure transducer. The parameters of gas kinetics and cumulative gas production were not altered (P > 0.05), except for gas production rate constant for the insoluble fraction which was the highest at 11.7 mg DR seed meal inclusion. CH4 production and total protozoa counts linearly decreased with increasing DR seed meal levels (P < 0.05). DR seed meal addition caused quadratic increase of in vitro DM digestibility with the highest value at 11.7 mg DR seed meal inclusion. No significant difference in volatile fatty acid profile (P > 0.05) was stated between treatments except for propionic acid. In conclusion, supplementation of DR seed meal resulted in improved in vitro gas kinetics and DM digestibility up to 11.7 mg level, while CH4 production was reduced linearly. The further in vivo studies are necessary to examine practical of DR seed meal usage in animal production.
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Monitoring of draft beer fermentation process by electronic nose
(2014-01-01)
Phetchakul, Toempong
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Sutthinet, Chalin
The draft beer fermentation process was monitored by using electronic nose. The system used the seven types of generally commercial metal oxide gas sensor in array. The selected process was one of the commercial processes and the data were gathered every hour for 10 day process which was 240 steps. It was processed for monitoring the chemical reaction for quality control. The results showed the good difference of the chemical reaction of the each day very clearly. The signature processing showed the variance 98.78%, 96.55% of the first component (PC1) and 2.3% of the second component (PC2), which was sufficiency for data explanation in 2D plot. It would be useful for production process to ensure a quality standard. © (2014) Trans Tech Publications, Switzerland.
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