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ZNO AND TIO2 nanoparticles stimulate callus induction and secondary metabolite production in indica RICE
(2020-01-01)
Chutipaijit, Sutee
;
Sutjaritvorakul, Thanawat
Metal nanoparticles are widely used in various fields of industry and their impact in the agricultural field is increasing. This research was carried out to investigate the effects of ZnO and TiO<inf>2</inf> nanoparticles on callus induction and plant metabolism in indica rice. Indica rice was assessed in plant tissue culture condition to estimate the influence of metal nanoparticles on morphological and physiological factors over 4 weeks. The seeds of Oryza sativa L. var. KDML105 were exposed to 1-3 mg L-1 of 2,4-dichlorophenoxyacetic acid (2,4-D) in the callus induction medium. The induced-calli were cultured on the callus induction medium supplemented with 2 mg L-1 of 2,4-D and showed a high increase in size and dry weight contents under the light condition when compared with the control and other treatments. Moreover, this research also investigated the potential impacts of ZnO and TiO<inf>2</inf> nanoparticles at 200, 400 and 800 mg L-1 on morphological and metabolic responses of the induced-calli cultured on the callus induction medium supplemented with 2,4-D and each type of metal nanoparticles. At 4 weeks after treatment, the induced-calli were analyzed for plant growth and assays on aspects of their metabolism were undertaken. The induced-calli exposed to 200 mg L-1 of ZnO or TiO<inf>2</inf> nanoparticles were found to have significant increase of biomass (size and dry weight of callus), total phenolic compounds and total flavonoid contents in KDML105 variety. The results demonstrated that utilization of ZnO and TiO<inf>2</inf> nanoparticles at appropriate concentration could positively induce the production of beneficial secondary metabolites in the induced-calli of indica rice and are worthy of further study for applications in other plant species.
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Titanium dioxide (TiO2) nanoparticles induced callus induction and plant regeneration of indica rice cultivars (suphanburi1 and suphanburi90)
(2018-10-01)
Chutipaijit, S.
;
Sutjaritvorakul, T.
Efficient plant regeneration systems are useful for indica rice using micropropagation and/or genetic transformation. Nanomaterials provide applications and opportunities in various fields of life science and agriculture technology. In this research, indica rice cultivars ‘Suphanburi1’ and ‘Suphanburi90’ (Oryza sativa L. ssp. indica) were used to investigate. Seeds were examined to evaluate callus induction and plant regeneration frequency. Culture of sterilized-seeds on callus induction medium supplemented with 50 mg L-1 of TiO<inf>2</inf> nanoparticles induced a high frequency of callus induction and content of biomass accumulation. Embryogenic calli were transferred to plant regeneration medium supplemented with 10-50 mg L-1 TiO<inf>2</inf> nanoparticles. The highest frequency of plant regeneration and the ratio of the number of seedlings to the number of regenerated calli were produced on plant regeneration medium supplemented with 40 mg L-1 of TiO<inf>2</inf> nanoparticles. The regenerated shoots induced roots on NB medium without plant growth regulators and the plantlets were established in soil. The results may be used for nanomaterial applications of rice and/or other plants for its further improvement.
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High contents of proline and anthocyanin increase protective response to salinity in Oryza sativa L. spp. indica
(2011-09-01)
Chutipaijit, Sutee
;
Cha-um, Suriyan
;
Sompornpailin, Kanokporn
Pigmented rice cultivar is a good candidate for abiotic tolerance especially salt tolerant trait. Physiological and growth parameters of eight indica rice genotypes under control or salt stress conditions were subjected to Hierarchical cluster analysis. From cluster ranking, rice genotypes were classified into two groups, salt-tolerant (Sangyod, Khao Dang, Kulab Dang and TD49) and salt-sensitive genotypes (Klum Sakol, Klum Khonkaen1, Klum Khonkaen2 and Black Sticky Rice). In salt stress (100 mM NaCl), the salt-tolerant genotypes showed the high percentages of proline and anthocyanin more than in the salt-sensitive genotypes. In addition, the photosynthetic pigment stabilizations of stressed-seedlings were positively correlated to the proline (R 2 = 0.98) and anthocyanin (R 2 = 0.70) accumulations. While, the malondialdehyde (MDA) contents of salt-stressed seedlings were negatively correlated to the proline (R 2 = 0.63) and anthocyanin (R 2 = 0.51) accumulations. Moreover, the total antioxidant activity of extraction from stressed-seedlings was assessed using the ABTS (2,2-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid) method. The higher percentages of total antioxidant activity were determined in salt-tolerant (125-199%) than salt-sensitive genotypes (106-113%). From the results it can be concluded that accumulation of above mentioned metabolites is associated with cellular protection and salt detoxification in salinity rice seedlings.
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Proline accumulation and physiological responses of indica rice genotypes differing in tolerance to salt and drought stresses
(2010-06-01)
Chutipaijit, Sutee
;
Suriyan, Cha Um
;
Sompornpailin, Kanokporn
The study determined the relationship between proline accumulation and physiological responses of indica rice genotypes to salt and drought stresses in photoautotrophic conditions. Two salt-tolerant genotypes of indica rice [cv. jasmine rice (KDML 105) and Dang], two drought-tolerant genotypes [Rice Department 6 (RD6) and Rice Department 15 (RD15)] and a salt-and drought-sensitive genotype [Pathumthani 1 (PT 1)] were exposed to 100 mM NaCl (salt stress) or 100 mM mannitol (drought stress) for 4 d. The relative water content (RWC) and malondialdehyde (MDA) accumulation in salt-and drought-tolerant genotypes did not significantly change. The proline accumulation in these genotypes greatly increased with stress. On the other hand, the salt-and drought-sensitive genotype which had lower proline accumulation demonstrated a significant decrease in RWC and increase in MDA content. Moreover, our results showed a strong correlation between proline concentrations and free radical scavenging by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay. The high concentration of proline solution presented high DPPH antioxidant activity. The higher proline accumulation in stressed seedlings of tolerant genotypes may help to maintain the RWC and MDA of rice plant, which could lead to preferable growth performance.

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