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    Selection for Upland Rice Varieties Under Different Levels of Light Intensity
    (2022-04-01)
    Nokkoul, Raumjit
    ;
    Unsup, Karnchanaporn
    ;
    Krutsuwan, Apichart
    ;
    Wichitparp, Thirayut
    Upland rice varieties were selected under varying levels of light intensity. This study identified shade-tolerant upland rice varieties with a high yield. From November 2016 to March 2017, the experiment was conducted at King Mongkut's Institute of Technology in Ladkrabang, Prince of Chumphon, Thailand. For this, ten varieties of upland rice i.e. Dawk Pa-yawm, Nangdam, Dokkam, Nangchuan, Lebnokrai, Samduen, Maepeung, Damgatondom, Lebmuenang, Pukaotong were grown at different levels of 0%, 50%, 60%, and 70% shading levels. The experiment was conducted in a randomized complete block design with four replications. The results of qualitative characteristics indicated that shadowing conditions didn’t have any significant effect on plant height, 50% flowering age, harvesting age, and panicle length. On the other hand, shaded conditions have a substantial effect on the number of plants per tiller, the number of panicles per tiller, the number of perfect grains per panicle, and the amount of empty grain per panicle since there was a quantitative character. Among the tested varieties, Lebmuenang, Pukaotong, Damgatondom, and Nangchuan varieties are suited for growing under 50% shade conditions while Samduen was only suited for cultivation in 70 % shaded conditions, whereas Pukaotong was good for growing in 50% and 60% shade situations. The results of this experiment did not encourage farmers to use the other varieties as cultivars under shaded conditions due to low yields. However, the outcomes of this study can be used as a reference for breeding programs in low-light conditions.
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    Effect of light intensity and light pattern on hydrogen production by unicellular green alga chlorella sp. LSD-W2
    (2019-01-01)
    Puangplub, Amornrat
    ;
    Phunpruch, Saranya
    Green microalgae can use solar energy and water to produce H2 via hydrogenase enzyme activity. The unicellular green alga Chlorella sp. LSD-W2 has been previously shown to produce high H2 under nitrogen deprivation. This research aimed to examine the effects of light intensity and light pattern on H2 production by Chlorella sp. LSD-W2 under nitrogen deprivation. The result showed that H<inf>2</inf> production rate was significantly enhanced when light intensities were increased. The cells could hardly produce H2 in the dark. The highest H2 production rate with 0.956 ± 0.015 mL L<sup>-1</sup> h<sup>-1</sup> was obtained in cells incubated in TAP-N medium in a 120-mL glass bottle under light intensity of 60 μmol photons m<sup>-2</sup> s<sup>-1</sup>. H<inf>2</inf> production by cells incubated under light/dark or dark/light cycles was lower than that under continuous light illumination. In order to reduce O2 which is an inhibitor of hydrogenase enzyme, the PSII inhibitor, 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU) was added to the Chlorella sp. LSD-W2 cell cultures. It was found that O<inf>2</inf> was obviously decreased in cells treated with 10 μM DCMU. Unexpectedly, DCMU caused the reduction of H<inf>2</inf> production by Chlorella sp. LSD-W2.