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    Research Article Biosynthesis of Silver Nanoparticles Using Garcinia cowa Aqueous Leaf Extract and Their Antifungal Activity Against Durian Dieback Pathogen
    (2023-09-01)
    Sukkha, Usa
    ;
    Khejonrak, Awadol
    ;
    Kamonpha, Phitsamai
    ;
    Ruangvittayanon, Anuchit
    ;
    Pakdeepromma, Sirichatnach
    Silver nanoparticles (AgNPs) were synthesized under different pH and reaction time using the Garcinia cowa aqueous leaf extract. The formation, crystal structure and morphology of synthesized AgNPs were investigated using UV-Vis spectroscopy, X-ray diffraction (XRD) and transmission electron microscopy (TEM) techniques. The UV-Vis result indicated that the AgNPs were synthesized using Garcinia cowa aqueous leaf extract at all pH conditions within 10 min. The synthesis of AgNPs was significantly affected by pH condition which was shown by the TEM micrographs. The particle sizes of AgNPs were in a nano-sized range; 6.30 ± 3.52 nm for pH 4, 3.87 ± 2.51 nm for pH 7 and 13.97 ± 11.84 nm for pH 10. Furthermore, the AgNPs prepared in pH 4, pH 7 and pH 10 (120 min reaction time) at different concentrations were assessed for their antifungal activity against durian dieback pathogen. It was found that the 800 mL/L of AgNPs prepared at pH 7, the smallest particle size and good dispersion, showed the highest inhibition mycelial growth at 47.33%, whereas the larger nanoparticles prepared at pH 4 and pH 10 conditions showed lower inhibition of mycelial growth at 43.22% and 32.55%, respectively.
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    MYCO-MEDIATED SYNTHESIS AND α-GLUCOSIDASE INHIBITORY ACTIVITY OF SILVER NANOPARTICLES PRODUCED BY XYLARIACEOUS FUNGI
    (2023-01-01)
    Sutjaritvorakul, T.
    ;
    Imsuwan, P.
    ;
    Damsud, T.
    ;
    Meksiriporn, B.
    ;
    Chutipaijit, S.
    Metal nanomaterials could be applied in various fields and could be synthesized via living organisms such as plants, bacteria and fungi. Here, the ability of Xylaria sp.5 to produce silver nanoparticles (AgNPs) and α-glucosidase inhibitory activities by the AgNPs produced by Xylaria sp.5 were investigated. The culture broth of Xylaria sp.5 was used to synthesize AgNPs by using 0.1 M of silver nitrate (AgNO3) solutions. The mycogenic crystals were investigated for the morphological characteristics and chemical composition by scanning electron microscope (SEM) equipped with energy dispersive X-ray spectroscopy (EDS), Fourier-transform infrared spectroscopy (FTIR) and X-ray powder diffraction (XRD). The results showed that AgNPs were successfully produced. Moreover, AgNPs were tested for α-glucosidase inhibitory activities. The crude enzyme derived from rat intestine consisted of maltase and sucrase. The percentage of inhibition at 50 mg/ml of maltase and sucrase was 63.21 ± 0.67 and 54.42 ± 0.11, respectively. This study demonstrated that the supernatant culture broth of Xylaria sp.5 can be used to synthesize AgNPs which possess α-glucosidase inhibitory activities. Collectively, this method could be a promising alternative for low cost and non-polluting production of AgNPs which could be potentially utilized for the treatment of type 2 diabetes mellitus.