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Fluorescence based rapid E. coli Detector
(2021-01-01)
Chantharasuriyasakun, Thitiyo
;
Sungwol, Siriyakorn
;
Piyawattanametha, Wibool
We have developed a fluorescence based rapid detection for E. coli which is an indicator for water quality identification. This portable detector will trim down the time taken to detect E. coli in the water from a few days to just a couple of minutes. Moreover, with the use of enzyme-substrate reaction between the enzyme β-D glucuronidase (GUD) in the E. coli and the substrate 4-methylumblliferyl-β-D glucuronide (MUG) resulting in a byproduct of 4-methylumbellliferone (4MU), the fluorescence emitting from this byproduct is then detected by our system and be enumerated for the number of E. coli. Hence, we have tested our system with two different pH solution, distilled water and tap water with pH values at 6.68 and 7.81 consecutively. Our developed system can detect the byproduct of 4MU in the concentration range of 0.001 μ M to 2 μM for the distilled water and 0.001 μM to 0.1 μM for the tap water, which can then be used for the enumeration of E. coli.
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Evaluation of rapid detection system for Escherichia coli in water samples
(2017-02-21)
Pipitsombat, Chanikarn
;
Phasuk, Kanchana
;
Suwan, Supakorn
;
Piyawattanametha, Wibool
We demonstrated a fast detection platform for Escherichia coli (E. coli) in water. The system is based on a fluorescence detection technique by determining enzyme β-D-glucuronidase (GUD) activity and relating that activity to E. coli concentration. We found the portable E. coli detection platform is able to detect both the fluorescence 4-MU signal from the reaction of GUD assay from 0.01-1 μg/ml and to enumerate the number of E. coli in the range of 10<sup>5</sup>-10<sup>7</sup> CFU/ml. The required incubation time is in between 20-240 min. In addition, we can estimate the maximum of incubation time for detecting minimum of E. coli 1 CFU/ml from the equation to be 576 mins. Our preliminary study demonstrates that the system can be used to enumerate the number of E. coli in water on-site and requires less detection time than traditional E. coli methods.
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Wide-field high-resolution fiber bundle based endomicroscope for cervical epithelium cells imaging
(2017-02-21)
Dumripatanachod, Manatchakorn
;
Piyawattanametha, Wibool
Endomicroscope is commonly used in epithelium diagnosis. We have designed a low-cost wide-field high-resolution fiber bundle based endomicroscope with off-The-shelf optical components and a commercial CMOS camera. We compensated the low sensitivity of the CMOS sensor in the camera with a suitable magnification of the optical system and image processing algorithm which is running on an embedded system. In this paper, the prototype of the designed endomicroscope can resolve a spatial resolution of 70 microns and capable of fluorescence imaging.
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A portable escherichia colil detection platform for water inspection
(2016-02-04)
Pipitsombat, Chanikarn
;
Piyawattanametha, Wibool
A large proportion of the population of Thailand relies on stored rainwater or coin water dispensers show in figure 1 as their main sources of drinking and household use water. There are over 60,000 coin water dispensers as shown in figure 1 (>50,000 in Bangkok and >10,000 in other provinces) alone. Substandard in drinking water quality has long been a major issue due to no regular water testing even with regulatory mandates. Little data are available on the microbial quality of these water sources. This data gap is partly due to the high cost and technical difficulty of current water quality testing methods. There are more than 20 methods to measure and E. coli in water. The major issues with these methods are they are time consuming, labour intensive, and give retrospective information, often when it is too late to take corrective action. Most of these methods are lab-based and take at least 24 hrs to produce a result after the sample reaches the laboratory. There is an urgent need at many levels in the water supply chain to minimize contaminating E. coli. Monitoring for E. coli itself is routinely done in assessing water quality as they are a good indication on the presence of pathogens, and microbiology of, a given supply, and thus, in themselves, they are a good measure of overall water quality. In this work, our team will introduce recent developments of E. coli detection with portable systems and show our preliminary approach to develop a cost effective E. coli in water detection system with processing time less than 4 hrs based on a specific enzymatic detection (β-D glucuronidase) which is widely used for the detection and enumeration of E. coll.

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