Dejtisakdi, Wipawee

Preferred name

Dejtisakdi, Wipawee

Alternative Name

Dejtisakdi, W.

Main Affiliation

School of Science

wipawee.de@kmitl.ac.th

Now showing 1 - 7 of 7

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Genome Insights into the Plant Growth Promoting Features of a Newly Found Microbispora sp. SCL1-1
(2024-10-02)
Dejtisakdi, Wipawee
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Duangupama, Thitikorn
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Thawai, Chitti
Plant growth-promoting (PGP) actinobacteria can be used to promote plant growth. Their use is a promising strategy that can be employed instead of agricultural chemical fertilizers. An actinobacterium strain, designated SCL1-1, was collected and isolated from a soil sample in a herbal garden at Pathum Thani province, Thailand. Analysis revealed that the SCL1-1 strain was a Gram-positive bacterium that formed longitudinal paired spores that were borne directly on aerial mycelia. It contained meso-diaminopimelic acid in its cell wall peptidoglycan. Moreover, madurose, which is a diagnostic sugar, was present in its whole-cell hydrolysates. 16S rRNA gene analysis revealed that the SCL1-1 strain was a member of the Microbispora and showed a close relationship to Microbispora rosea ATCC 12950T (99.6%), followed by Microbispora hainanensis DSM 45428T (99.2%). However, a genome-based polyphasic study revealed that strain SCL1-1 had a low average nucleotide identity (ANI) (<95%), and digital DNA–DNA hybridization (dDDH) value (<70%) with M. rosea ATCC 12950T and M. hainanensis DSM 45428T, indicating that strain SCL1-1 was a different species to its close relatives. Genome mining of strain SCL1-1 showed the presence of genes related to the production of indole-3-acetic acid (IAA), and siderophore, which are agents that promote plant growth. In addition, the genome of strain SCL1-1 was found in several secondary metabolite biosynthetic gene clusters, which were possibly encoded for a broad range of remarkable natural products and antibiotics.
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Lactobacillus plantarum JCM 1149 Growth Enhancement by using Chlorella sp. KLSc61-pretreated Cells
(2025-06-01)
Khanrin, Lalita
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Boonyakorn, Phonwimon
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Wongsariya, Karn
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Kittiwongwattana, Chokchai
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Maneeruttanarungroj, Cherdsak
Dry microalgal biomass was previously tested as prebiotic to enhance the growth of probiotic bacteria. However, the drying process could be ineffective for scaling up probiotic production. This study aimed to investigate the use of fresh, pretreated microalgal biomass to promote the growth of Lactobacillus plantarum JCM 1149. Chlorella sp. KLSc61 cells were pretreated by three different methods: physical treatment with microwave radiation at power levels of 300, 500, and 700 W; chemical treatment with 0.1 M citric acid and 0.5 M sodium hydroxide; and biological treatment with cellulase enzyme. The 2.5% pretreated Chlorella cells were then added to L. plantarum JCM 1149 culture, and the growth was observed at 37 °C for 24 h of incubation. The results showed that, during the log phase (6-10 h), Chlorella cells pretreated with microwave radiation at 700 W were the most effective in promoting L. plantarum JCM 1149 growth, which was 1.4- and 1.5-fold of L. plantarum JCM 1149 without adding Chlorella and with untreated cells, respectively. Extension of the pretreatment time by microwave radiation at 700 W from up to 2 min increased the growth of L. plantarum JCM 1149 up to 1.8-fold of pretreatment time by microwave radiation at 700 W 1 min, compared to the control groups. Additionally, increasing the amount of Chlorella biomass up to 5% (w/v) extended the log phase of L. plantarum JCM 1149 and increased cell accumulation during the stationary phase. Unlike dry microalgal biomass, the simplicity of fresh, pretreated Chlorella biomass shown in this study may facilitate large-scale, commercial production of L. plantarum strains as probiotics.
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Using Phytoplankton as Bioindicators of Tourism Impact and Seasonal Eutrophication in the Andaman Sea (Koh Yaa, Thailand)
(2026-01-01)
Wongsnansilp, Tassnapa
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Khamcharoen, Manoch
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Boonrong, Jaran
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Dejtisakdi, Wipawee
This study focuses on the diversity of phytoplankton in the Koh Yaa region of Thailand and their relationship with environmental variables, aiming to assess whether human activities (primarily tourism) pose potential threats to the marine ecosystem and provide scientific support for eco-sustainable tourism management decisions in the region. In April, August, and December 2024, corresponding to peak season, off-season, and shoulder season, a total of 156 discrete samples were collected from four coastal sites to analyze water quality parameters such as temperature, pH, total nitrogen (TN), and total phosphorus (TP), along with plankton diversity and abundance. Statistical analyses including two-way ANOVA with Duncan’s Multiple Range Test (DMRT), Pearson correlation analysis, and principal component analysis (PCA) were applied. The results showed a declining trend in plankton abundance over time, peaking at 1009 × 106 cells/m3 in April and dropping to 281 × 106 cells/m3 by December. A total of 15 types of phytoplankton were identified across four phyla: Bacillariophyta, Cyanobacteria, Dinoflagellata, and Chlorophyta. Notably, Chaetoceros from Bacillariophyta accounted for 47% of phytoplankton, while Oscillatoria from Cyanobacteria made up 29.6%. The diversity index and evenness index improved from 1.34 and 0.46 in April to 1.88 and 0.64 in December, respectively. Environmental factors like pH, temperature, and TP significantly affected phytoplankton abundance (p < 0.01), with TP levels ranging from 0.27 to 0.69 mg/L. These results indicate possible pollution in this region, and changes in phytoplankton abundance were linked to seasonal climate variations—especially during peak tourist seasons—which may exacerbate eutrophication affecting community structures.
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Outdoor cultivation of Dunaliella salina KU 11 using brine and saline lake water with raceway ponds in northeastern Thailand
(2017-11-01)
Wu, Zhe
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Dejtisakdi, Wipawee
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Kermanee, Prasart
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Ma, Chunhong
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Arirob, Wallop
To evaluate the potential of algal biotechnology to replace traditional agriculture in northeastern Thailand, an open raceway cultivation system was developed to produce biomass and beta-carotene. Dunaliella salina KU 11 isolated from local saline soil was cultured in open raceway tanks using brine and saline lake water. Grown in modified Johnson's medium (with 2–3.5 M NaCl), the algae reached a maximum cell density on the fourth day (1.8 × 106 cells mL−1). Increasing KNO<inf>3</inf> and NaHCO<inf>3</inf> from 0.5 and 0.043 g L−1 to 1 and 2.1 g L−1, respectively, significantly improved the yields of biomass (0.33 g L−1) and beta-carotene (19 mg L−1). Expected profits for algal production were evaluated, and it was found that this strain was suitable for outdoor cultivation and the developing algal industry in northeastern Thailand could produce high economic benefits (at least $64,120 per year per 0.16 ha).
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Optimal conditions for maximized H2 yield from a new green algal strain Chlorella sp. KLSc61
(2022-08-01)
Laokua, Natwikar
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Rittiyan, Nutnicha
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Kornrawudaphikasama, Yosita
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Klinsalee, Rachaneekorn
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Tonawut, Yothawut
Hydrogen produced from microalgae is attracting the attention of scientists as a potential new ‘Bio-Circular-Green’ energy source. We screened a selection of naturally occurring algal strains for H<inf>2</inf> yield as a basis for high yield development. The best strain was a new strain of biohydrogen-producing green algae from a fresh water source at King Mongkut's Institute of Technology Ladkrabang, Thailand. Using morphological and 18S rDNA sequencing analyses, this alga was identified and classified as Chlorella sp. KLSc61. When adapted cells in TAP-K medium (with starting pH at 9.0) containing 25 mM ethanol, with 54 μmol photons m−2 s−1 light, and incubated at 35 °C, Chlorella sp. KLSc61 showed a maximum H<inf>2</inf> yield of 9,804 ± 469.5 μmol H<inf>2</inf> mg−1 Chl at day 7. Under these conditions, the cells produced ~ 12.2 times more H<inf>2</inf> gas than without optimization (753.0 μmol H<inf>2</inf> mg−1Chl). Our optimized system also produced H<inf>2</inf> at a rate 11–570 times greater than previously reported microalgae. Thus Chlorella sp. KLSc61 could be a viable strain for biohydrogen production as a clean future energy source.
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New Synthetic Operon Vectors for Expressing Multiple Proteins in the Chlamydomonas reinhardtii Chloroplast
(2023-02-01)
Yeon, Jihye
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Miller, Stephen M.
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Dejtisakdi, Wipawee
Microalgae are a promising platform for generating valuable commercial products, including proteins that may not express well in more traditional cell culture systems. In the model green alga Chlamydomonas reinhardtii, transgenic proteins can be expressed from either the nuclear or chloroplast genome. Expression in the chloroplast has several advantages, but technology is not yet well developed for expressing multiple transgenic proteins simultaneously. Here, we developed new synthetic operon vectors to express multiple proteins from a single chloroplast transcription unit. We modified an existing chloroplast expression vector to contain intercistronic elements derived from cyanobacterial and tobacco operons and tested the ability of the resulting operon vectors to express two or three different proteins at a time. All operons containing two of the coding sequences (for C. reinhardtii FBP1 and atpB) expressed the products of those genes, but operons containing the other two coding sequences (C. reinhardtii FBA1 and the synthetic camelid antibody gene VHH) did not. These results expand the repertoire of intercistronic spacers that can function in the C. reinhardtii chloroplast, but they also suggest that some coding sequences do not function well in the context of synthetic operons in this alga.
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Proteomic insights into high biohydrogen production by Chlorella sp. KLSc61 under potassium deprivation: upregulation of carbohydrate synthesis proteins
(2025-12-01)
Laokua, Natwikar
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Dejtisakdi, Wipawee
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Maneeruttanarungroj, Cherdsak
Microalgae produce hydrogen by utilizing light energy to split water molecules and this produced hydrogen is considered as a promising alternative energy resource. This study investigated the proteomic response of the microalga, Chlorella sp. KLSc61 under an optimal condition for increasing hydrogen production. Chlorella KLSc61 cells were cultured in potassium deprivation medium (TAP-K medium) with an initial pH of 9.0 and added 25 mM ethanol, they were cultivated and exposed to the light intensity of 54 μmol photons m−2 s−1 at 35 °C. By day 7, under this growth condition, Chlorella cells produced a maximum H<inf>2</inf> yield of 19,600 ± 0.3 mmol H<inf>2</inf> mg−1 chlorophyll, which was 2.5 times greater than that under normal TAP condition. Under an increase of hydrogen production, Chlorella KLSc61 cells were both round- and oval-shaped with an average cell diameter of 10.0 ± 0.5 μm, along with variations of chloroplast distribution within the cytoplasm, whereas Chlorella cells in normal TAP medium showed only round shape with an average cell diameter of 5.0 ± 0.5 μm. Proteins from cells grown under normal and high hydrogen conditions were subjected to proteomic analysis, with the results presented as a cluster heat map of proteomics profiles from five different conditions. A total of 736 protein expression patterns were classified into six different expressed protein groups, which included both up- and down-regulated proteins. Carbohydrate synthesis proteins, including starch synthase, glucose-6-phosphate isomerase, glycerol-3-phosphate dehydrogenase, phosphoglucomutase, and UDP-glucuronate decarboxylase, were abundant during the high H<inf>2</inf> production. These findings allow us to take the next step of those protein overexpression into Chlorella KLSc61 cells to enhance in vivo hydrogen production and this strain could be used as a hydrogen production platform.

Dejtisakdi, Wipawee

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