Poeaim, Supattra

Preferred name

Poeaim, Supattra

Alternative Name

Poeaim, S.

Poeaim, Supattar

Main Affiliation

School of Science

supattra.poe@kmitl.ac.th

Now showing 1 - 8 of 8

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Efficient rice straw saccharification by enzyme extract from Pseudolagarobasidium acaciicola TDW-48 and recycling its solid residue as a green and novel support for laccase immobilization
(2025-02-01)
Luong, Thi Thu Huong
;
Poeaim, Supattra
This study aimed to provide a novel and feasible approach for rice straw bioconversion regarding the combination of enzymatic saccharification and recycling of its solid residue for enzyme immobilization. Firstly, rice straw was saccharified by Pseudolagarobasidium acaciicola TDW-48's enzyme extract and then optimized to improve its performance. Next, the enzymatic-degraded rice straw (a solid residue from saccharification) was recycled as an enzyme support for laccase immobilization. The result indicates that the rice straw saccharification by P. acaciicola TDW-48's enzyme extract was a practical process for biofuel and other value-added product production. It reached 13.4 g/L of reducing sugar concentration under optimum conditions: 12 h of incubation time, 43.22 °C of temperature, pH of 4.5 and substrate loading of 0.48 g. In the next regard, enzymatic-degraded rice straw under adsorption-crosslinking mode was a promising novel support for laccase immobilization. This process achieved 1.24 U/gsupport of immobilized laccase activity and 88.3% in immobilization yield. The immobilized laccase could maintain 96.71% activity after 4 cycles and 53% activity after 10 cycles with ABTS substrate, and its stability was enhanced compared with free form. In addition, the immobilized laccase could decolorize 0.14 mg of bromophenol blue after 3 h and retain 53% relative efficiency after 6 cycles under biocatalyst bag form. This study encouraged the recycling of solid residue from rice straw saccharification, improving rice straw bioconversion. Moreover, it provided green, novel and economic support for enzyme immobilization with high applicability and simplicity.
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Isolation and Characterization of a Promising Lignocellulolytic Enzyme Producer Pseudolagarobasidium acaciicola SL3-03 from Mangrove Soil in Thailand
(2025-01-01)
Jadtanim, Chanaphon
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Luong, Thi Thu Huong
;
Poeaim, Supattra
Lignocellulolytic enzymes isolation from mangrove-derived organisms has many industrial advantages due to their efficiency in dealing with extreme and challenging conditions, such as high temperatures and salt concentrations. This study aimed to isolate fungal enzyme producers from mangrove soil in Thailand to produce lignocellulolytic enzymes (carboxymethyl cellulase: CMCase, xylanase, and laccase) and to characterize these enzymes to support industrial applications. Forty-eight fungi were isolated from the mangrove samples, and their enzyme-producing capabilities were assessed using primary and secondary screening methods. The findings revealed that Pseudolagarobasidium acaciicola SL3-03 emerged as a promising producer of lignocellulolytic enzymes. It exhibited the ability to produce 1.345 U/mL of CMCase, 1.293 U/mL of xylanase, and 43.126 U/mL of laccase. Furthermore, the enzymatic characteristics of P. acaciicola SL3-03 were analyzed. The CMCase exhibited optimal activity at 50 °C and pH 5.5, the xylanase at 50 °C and pH 4.8, and the laccase at 55 °C and pH 5. Besides, the CMCase and xylanase from P. acaciicola SL3-03 expressed high halotolerance abilities that could maintain activity and stability under high salt concentrations (149% activity at 5 M NaCl). Future studies may focus on structural analysis of the enzymes to further characterize and identify their specific types. The results suggest that mangrove soil harbors significant potential for discovering proficient lignocellulolytic enzyme producers with desirable characteristics, which can be advantageous for industrial applications.
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Isolation, screening wood rot fungi from the tropical forest of Thailand and their lignocellulolytic enzyme production under solid-state fermentation using agricultural waste as substrate
(2025-01-01)
Luong, Thi Thu Huong
;
Silar, Philippe
;
Poeaim, Supattra
;
Tangthirasunun, Narumon
The present study aimed to discover good lignocellulolytic enzyme (LCE) producers from Thailand’s tropical forest and then examine their multiple LCE production (including carboxymethyl cellulase (CMCase), xylanase, and laccase) using agricultural wastes as substrate. The total collection was 50 fungi, mainly from the Polyporales, Agaricales, and Xylariales orders. During primary screening by qualitative method and secondary screening by quantitative method, two potential fungi were proposed for multiple LCE production, including Auricularia auricula-judae 088 and Pseudolagarobasidium acaciicola TDW-48. Under solid-state fermentation (SSF) using agricultural wastes as substrates, P. acaciicola TDW-48 performed as a good producer that highly secreted simultaneous CMCase, xylanase, and laccase. In the next stage, the simplex lattice mixture design assessed the interaction of agricultural waste substrates and their effects on P. acaciicola TDW-48’s enzyme production. The results indicated that agricultural waste has different influences on CMCase, xylanase, and laccase production: orange peel showed a positive effect on both CMCase and xylanase activity, but a negative effect on laccase. In contrast, wheat bran positively influenced laccase, while it limited CMCase and xylanase. However, the combination of these substrates in the mixture showed synergic effects and improved enzyme activity. Through numerical optimization, a ternary mixture of wheat bran (1.27 g), orange peel (1.53 g), and rice husk (0.2 g) was identified as the most appropriate formulation for simultaneous multiple LCE production, reaching 20.96 U/g substrate for CMCase, 23.94 U/g substrate for xylanase, and 27.55 U/g substrate for laccase. These results provided a promising candidate for LCE production with high applicability in lignocellulose bioconversion and successfully demonstrated the relationship between the agricultural waste substrate and multiple LCE production that supported the enzyme production following the environmentally friendly and economical approach.
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Isolation and Characterization of Xylanase from a Novel Strain, Penicillium menonorum SP10
(2023-01-01)
Luong, Thi Thu Huong
;
Poeaim, Supattra
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Tangthirasunun, Narumon
Xylanase has been applied in various sectors, such as biomass conversion, paper, pulp, textiles, and pharmaceutical industries. This study aimed to isolate and screen potential xylanase-producing fungi from the soil of Suphan Buri Province, Thailand. Fifteen fungi were isolated, and their xylanase activities were tested by the qualitative method. The result showed that isolate SP3, SP10 and SP15 gave high xylanase activity with potency index (PI) of 2.32, 2.01 and 1.82, respectively. These fungi were selected for the xylanase quantitative test, isolate SP10 performed the highest xylanase activity with 0.535 U/mL. Through molecular methods using the β-tubulin gene, isolate SP10 was identified as Penicillium menonorum. The xylanase characteristics from P. menonorum SP10 were determined, including the xylanase isoforms and the optimum pH and temperature. The xylanase isoforms on SDS-PAGE indicated that P. menonorum SP10 produced two xylanases (45 and 54 kDa). Moreover, its xylanase worked optimally at pH 6 and 55 °C while reaching 61% activity at 65 °C. These results proposed P. menonorum SP10 as a good candidate for industrial uses, especially in poultry feed and pulp industries, to improve yield and economic efficiency under slightly acidic and high-temperature conditions.
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Three-phase partitioning (TPP) purification, characterization and dye decolorization application of laccase from Pseudolagarobasidium acaciicola TDW-48
(2026-02-01)
Luong, Thi Thu Huong
;
Poeaim, Supattra
This study focused on the efficient and economical purification of laccase from P. acaciicola TDW-48 and its application in dye decolorization. Firstly, the P. acaciicola TDW-48's laccase was purified by the three-phase partitioning (TPP) method, and then the artificial neural network-genetic algorithm (ANN-GA) optimization was used to improve the purification yield. The result indicated that the TPP method successfully purified P. acaciicola TDW-48's laccase with a high purification yield. After ANN-GA optimization, the strong interactive effects of the TPP parameters on purification yield were demonstrated by a high-accuracy ANN model (R-value of 0.99918 for all datasets). An optimum TPP system was developed, achieving 138.7 % activity recovery and 1.62-fold purity at a 57.82 % salt concentration, pH 5.75, and a t-butanol/enzyme ratio of 1.5. In the following, the enzyme characteristics and application potential of purified laccase were determined. The P. acaciicola TDW-48's laccase showed a molecular weight of 60.5 kDa and functioned optimally at pH 3 and 30 °C. The kinetic parameters implied high affinity and catalytic efficiency for the ABTS (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid)) substrate, with a low Km (37.9 μM) and a high Vmax (46.01 mM/min). Moreover, the purified laccase showed excellent potential for dye decolorization, with 44 % Congo red, 80 % bromophenol blue, and 58 % phenol red decolorized after 8 h of treatment.
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Multi-objective optimization of lignocellulolytic enzyme cocktail production from Pseudolagarobasidium acaciicola TDW-48 by artificial neural network-genetic algorithm (ANN-GA) strategy and its application in lignocellulose waste bioconversion
(2025-03-01)
Luong, Thi Thu Huong
;
Poeaim, Supattra
A massive amount of lignocellulose waste is generated annually, causing many environmental concerns. The bioconversion of these wastes into value-added products by the lignocellulolytic enzymes (LCE) is one of the effective and environmental approaches. However, the use of LCE has not been extended due to high costs. This study aimed to enhance the yield of crude LCE cocktail production from Pseudolagarobasidium acaciicola TDW-48 by optimizing cultural conditions using statistical tools. Firstly, the effect of cultural factors on LCE production was identified through the Plackett-Buman design. Then, the artificial neural network-genetic algorithm (ANN-GA) strategy was applied to optimize the significant factors. The result shows that the production of carboxymethyl cellulase (CMCase), xylanase, and laccase responded differently to cultural conditions. Among these, five factors (incubation time, water content, medium pH, glucose, and CuSO<inf>4</inf> concentration) were identified to have significant effects on enzyme activities. The ANN-GA optimization with a neuron network architecture (5-23-3) successfully modeled the crude LCE cocktail production, where the R-value achieved 0.98369 for the total dataset. A set of optimum conditions was proposed with an incubation time of 8 days, 72.6% water content, medium pH at 2.97, 0.5% glucose, and 0.53 g/L of CuSO<inf>4</inf>. With the above conditions, P. acaciicola TDW-48 could produce 23.97 U/g of CMCase, 26.02 U/g of xylanase, and 139.11 U/g of laccase, which enhanced 14.4%, 8.7%, and 405% activity, respectively, compared with non-optimization. In addition, the P. acaciicola TDW-48’s crude LCE cocktail performed a high bioconversion efficiency on lignocellulose waste, the reducing sugar yield achieved 327.29 mg/g on rice straw, 308.02 mg/g on rice husk, and 312.29 mg/g on corn stover after 8-h incubation. These results provided a highly effective approach for LCE production with multi-objective optimization based on an artificial intelligence platform and supported the reuse of lignocellulose waste toward the eco-friendly strategy.
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USING AGRI-FOOD WASTES AS POTENTIAL SUBSTRATES FOR XYLANASE PRODUCTION BY PENICILLIUM MENONORUM SP10 AND ITS APPLICATION IN CORNCOB SACCHARIFICATION
(2025-01-01)
Luong, Thi Thu Huong
;
Prapasiri, Sarunpattori
;
Krachang, Cholthicha
;
Poeaim, Supattra
Xylanase is one of the key enzymes with wide spectrum application, especially in biomass saccharification. However, the xylanase production is still limited due to high processing costs. This study aimed to use agri-food wastes as substrates for Penicillium menonorum SP10’s xylanase production and investigate its capacity for corncob saccharification. The xylanase production was tested under solid-state fermentation using corncob, watermelon, passion fruit, lemon and orange peel substrates. Then, the corncob saccharification by P. menonorum SP10’s crude xylanase was optimized using a one-factor-at-time approach. The result indicates that P. menonorum SP10 could produce xylanase on agri-food waste substrates. The maximum xylanase activity was obtained on corncob with 45.35 U/mL after 7 days of incubation. Besides, P. menonorum SP10’s crude xylanase performed a high potential in corncob saccharification. It could release 6.08 mg/mL of reducing sugar under optimum conditions, including 0.4 g of substrate loading and temperature at 55℃ and pH 6 with an incubation time of 24 hours. Moreover, the hydrolysate’s sugar profile analysis on thin-layer chromatography indicated high glucose and xylose performances after saccharification. This study encouraged the reuse of agri-food wastes as cheap, abundant and nutrient substrates for xylanase production in the green, effective and economical direction.
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Biocontrol of Panama disease and postharvest anthracnose on bananas by Neosartorya and Talaromyces fungi
(2026-01-01)
Poeaim, Supattra
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Luong, Thi Thu Huong
;
Pattarasaikul, Wutthipong
This study aimed to propose a potential antagonist for controlling both Panama disease and postharvest anthracnose in bananas. The findings revealed that Fusarium oxysporum and Colletotrichum musae were the most virulent pathogens causing Panama disease and postharvest anthracnose, respectively. Through dual culture tests, Talaromyces trachyspermus AWP08-01 was identified as a promising biocontrol agent against both F. oxysporum and C. musae, achieving 62.30% spore inhibition and 42.09%, respectively. Moreover, the ethyl acetate extract of T. trachyspermus AWP08-01 showed good antifungal activity. At a 1000-µg/mL concentration, it could inhibit 22.14% and 51.58% spore production of F. oxysporum and C. musae, respectively. By GC-MS analysis, the ethyl acetate extract from T. trachyspermus AWP08-01 was detected containing benzoic acid, hexadecanoic acid, heptadecane and linoleic acid. These findings enhance our understanding of banana disease management and suggest the potential of T. trachyspermus for further development in management both pre-harvest and post-harvest banana diseases.

Poeaim, Supattra

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